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Wnt1 Antibody (600-401-A37) in WB
Western blot using Rocklands affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).
Product Details
600-401-A37
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Marsupial,
Mouse,
Rhesus monkey,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
Target Information
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: murine mammary tumor virus integration site; Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)
Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1; WNT1
UniProt ID: (Human) P04628, (Mouse) P04426
Entrez Gene ID: (Human) 7471, (Dog) 486560, (Rhesus monkey) 709009, (Bovine) 540662, (Rat) 24881, (Mouse) 22408
embryonic axis specification
branching involved in ureteric bud morphogenesis
positive regulation of protein phosphorylation
cell-cell signaling
myoblast fusion
positive regulation of cell proliferation
response to wounding
positive regulation of lamellipodium assembly
negative regulation of cell-substrate adhesion
Wnt signaling pathway
spinal cord association neuron differentiation
diencephalon development
central nervous system morphogenesis
cerebellum formation
forebrain anterior/posterior pattern specification
midbrain-hindbrain boundary maturation during brain development
negative regulation of cell-cell adhesion
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
ubiquitin-dependent SMAD protein catabolic process
midbrain development
organ regeneration
T cell differentiation in thymus
cell proliferation in midbrain
inner ear morphogenesis
signal transduction in response to DNA damage
positive regulation of insulin-like growth factor receptor signaling pathway
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
cell fate commitment
negative regulation of fat cell differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
neuron fate determination
neuron development
positive regulation of sequence-specific DNA binding transcription factor activity
Spemann organizer formation
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
bone development
positive regulation of dermatome development
hepatocyte differentiation
cellular response to peptide hormone stimulus
hematopoietic stem cell proliferation
positive regulation of canonical Wnt signaling pathway
negative regulation of cell aging
beta-catenin destruction complex disassembly
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
embryonic brain development
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
myotube differentiation
metencephalon development
neuron differentiation
midbrain-hindbrain boundary development
negative regulation of cell differentiation
neuron fate commitment
signal transduction
multicellular organism development
organ morphogenesis
BMP signaling pathway
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