Invitrogen

YAP1 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

17 References

View all (64) YAP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

YAP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

YAP1 Antibody (PA1-46189) in ICC/IF

Immunofluorescence analysis of YAP1 was performed using 70% confluent log phase HeLa cells treated with serum starvation for 12 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with YAP1 Polyclonal Antibody (Product # PA1-46189) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Al... View More

YAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

YAP1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

YAP1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

YAP1 Antibody in Immunohistochemistry (IHC)

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YAP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of YAP1 was observed in differentiated somatic cell lines (HEK293, HeLa, U-2 OS, MDA-MB-231, SK-BR-3 and MCF-7) in comparison to lymphoid cell lines (Jurkat and Raji) using YAP1 antibody (Product # PA1-46189) in western blot. {RE}

Product Details

PA1-46189

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

2-10 µg

ChIP assay (ChIP)

1.5 µg/10^6 cells

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat, Zebrafish

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant YAP1 protein expressed in bacteria.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2219137

Product Specific Information

Suggested positive control: brain and kidney lysates, and HEK 293 cell lysate, 293 whole cell lysate, antigen standard for YAP1 (transient overexpression lysate), human kidney protein, human brain protein.

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Radiation therapy affects YAP expression and intracellular localization by modulating lamin A/C levels in breast cancer.

Frontiers in bioengineering and biotechnology

La Verde G,Artiola V,Pugliese M,La Commara M,Arrichiello C,Muto P,Netti PA,Fusco S,Panzetta V

PA1-46189 was used in Immunocytochemistry-immunofluorescence to elucidate the role of extracellular matrix stiffness and ionizing radiations on YAP regulation; and explored the behaviour of two different mammary cell lines, a normal epithelial cell line (MCF10A) and a highly aggressive and invasive adenocarcinoma cell line (MDA-MB-231) interacting with polyacrylamide substrates mimicking the mechanics of both normal and tumour tissues (∼1 and ∼13 kPa)..

Tue Sep 13 00:00:00 EDT 2022

Radiation therapy affects YAP expression and intracellular localization by modulating lamin A/C levels in breast cancer.

Frontiers in bioengineering and biotechnology

La Verde G,Artiola V,Pugliese M,La Commara M,Arrichiello C,Muto P,Netti PA,Fusco S,Panzetta V

Tue Sep 13 00:00:00 EDT 2022

Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation.

PloS one

Geiger F,Schnitzler LG,Brugger MS,Westerhausen C,Engelke H

PA1-46189 was used in Immunocytochemistry to study the influence of fiber orientation in the extracellular collagen matrix on invasion from a cancer cell spheroid.

Tue Apr 26 00:00:00 EDT 2022

Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation.

PloS one

Geiger F,Schnitzler LG,Brugger MS,Westerhausen C,Engelke H

PA1-46189 was used in Immunocytochemistry to study the influence of fiber orientation in the extracellular collagen matrix on invasion from a cancer cell spheroid.

Tue Apr 26 00:00:00 EDT 2022

Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing.

Pharmaceutics

Wu M,Matar DY,Yu Z,Chen Z,Knoedler S,Ng B,Darwish OA,Sohrabi S,Friedman L,Haug V,Murphy GF,Rinkevich Y,Orgill DP,Panayi AC

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunohistochemistry

Thu Oct 06 00:00:00 EDT 2022

Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing.

Pharmaceutics

Wu M,Matar DY,Yu Z,Chen Z,Knoedler S,Ng B,Darwish OA,Sohrabi S,Friedman L,Haug V,Murphy GF,Rinkevich Y,Orgill DP,Panayi AC

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunohistochemistry

Thu Oct 06 00:00:00 EDT 2022

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation.

Cell death and differentiation

Canu V,Donzelli S,Sacconi A,Lo Sardo F,Pulito C,Bossel N,Di Benedetto A,Muti P,Botti C,Domany E,Bicciato S,Strano S,Yarden Y,Blandino G

PA1-46189 was used in Western Blotting to uncover an oncogenic feedback loop, comprising miR-10b-3p, SPAG5, and YAP/TAZ/TEAD, which fuels the aberrant proliferation of breast cancer.

Sat May 01 00:00:00 EDT 2021

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation.

Cell death and differentiation

Canu V,Donzelli S,Sacconi A,Lo Sardo F,Pulito C,Bossel N,Di Benedetto A,Muti P,Botti C,Domany E,Bicciato S,Strano S,Yarden Y,Blandino G

PA1-46189 was used in Western Blotting to uncover an oncogenic feedback loop, comprising miR-10b-3p, SPAG5, and YAP/TAZ/TEAD, which fuels the aberrant proliferation of breast cancer.

Sat May 01 00:00:00 EDT 2021

PI3K Inhibitors Curtail MYC-Dependent Mutant p53 Gain-of-Function in Head and Neck Squamous Cell Carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ganci F,Pulito C,Valsoni S,Sacconi A,Turco C,Vahabi M,Manciocco V,Mazza EMC,Meens J,Karamboulas C,Nichols AC,Covello R,Pellini R,Spriano G,Sanguineti G,Muti P,Bicciato S,Ailles L,Strano S,Fontemaggi G,Blandino G

PA1-46189 was used in ChIP assay to investigate mutant p53-associated functions through gene set enrichment analysis in the Cancer Genome Atlas cohort of head and neck squamous cell carcinoma and in a panel of 22 head and neck squamous cell carcinoma cell lines.

Mon Jun 15 00:00:00 EDT 2020

PI3K Inhibitors Curtail MYC-Dependent Mutant p53 Gain-of-Function in Head and Neck Squamous Cell Carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mon Jun 15 00:00:00 EDT 2020

Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.

The Journal of international medical research

Li H,Kan B,Song L,Liu Y,Jian X

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Tue Sep 01 00:00:00 EDT 2020

Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.

The Journal of international medical research

Li H,Kan B,Song L,Liu Y,Jian X

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Tue Sep 01 00:00:00 EDT 2020

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Cell death & disease

Han Y,Zhang L,Yu X,Wang S,Xu C,Yin H,Wang S

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunohistochemistry

Fri May 10 00:00:00 EDT 2019

NSCLC depend upon YAP expression and nuclear localization after acquiring resistance to EGFR inhibitors.

Genes & cancer

McGowan M,Kleinberg L,Halvorsen AR,Helland Å,Brustugun OT

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

PIK3CA mutations as prognostic factor in squamous cell lung carcinoma.

Lung cancer (Amsterdam, Netherlands)

McGowan M,Hoven AS,Lund-Iversen M,Solberg S,Helland Å,Hirsch FR,Brustugun OT

PA1-46189 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that PIK3CA mutations may confer a survival advantage in early stage squamous cell lung cancers.

Sun Jan 01 00:00:00 EST 2017

The clinical significance of forkhead box protein A1 and its role in colorectal cancer.

Molecular medicine reports

Ma W,Jiang J,Li M,Wang H,Zhang H,He X,Huang L,Zhou Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Thu Sep 01 00:00:00 EDT 2016

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Sat May 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; yap; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

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Gene Aliases: AI325207; cb194; CH211-181P1.5-001; COB1; sb:cb194; si:ch211-181p1.5; si:dkey-3b8.3; wu:fc18c04; YAP; YAP1; YAP2; YAP65; YKI; Yorkie; zgc:158380; zYAP

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UniProt ID: (Human) P46937, (Mouse) P46938, (Zebrafish) Q1L8J7

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Entrez Gene ID: (Dog) 479465, (Human) 10413, (Rat) 363014, (Mouse) 22601, (Zebrafish) 561411

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Function(s)

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding chromatin binding transcription coactivator activity transcription corepressor activity protein binding protein C-terminus binding transcription regulatory region DNA binding proline-rich region binding transcription cofactor

Process(es)

cell morphogenesis vasculogenesis embryonic heart tube morphogenesis transcription initiation from RNA polymerase II promoter cellular response to DNA damage stimulus cell proliferation positive regulation of cell proliferation regulation of keratinocyte proliferation keratinocyte differentiation negative regulation of epithelial cell differentiation notochord development somatic stem cell population maintenance hippo signaling positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth paraxial mesoderm development lateral mesoderm development regulation of neurogenesis contact inhibition bud elongation involved in lung branching lung epithelial cell differentiation cellular response to retinoic acid cellular response to gamma radiation regulation of stem cell proliferation regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of canonical Wnt signaling pathway positive regulation of stem cell population maintenance negative regulation of nucleic acid-templated transcription negative regulation of stem cell differentiation negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter DNA damage induced protein phosphorylation regulation of cell proliferation regulation of canonical Wnt signaling pathway liver development secondary heart field specification ventriculo bulbo valve development retinal pigment epithelium development brain development endoderm development dorsal/ventral axis specification negative regulation of gene expression BMP signaling pathway pancreas development fin regeneration embryonic hemopoiesis pronephric duct development glucose homeostasis negative regulation of apoptotic process regulation of organ growth blood vessel morphogenesis posterior lateral line development cartilage development cilium morphogenesis heart formation positive regulation of glutamate-ammonia ligase activity

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YAP1 Polyclonal Antibody

YAP1 Antibody (PA1-46189) in ICC/IF

Product Details

PA1-46189

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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