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YAP1 Monoclonal Antibody (3A7A9)

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Antibody Testing Data (3)

YAP1 Antibody in Immunocytochemistry (ICC/IF)

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YAP1 Antibody (66900-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YAP1 antibody (66900-1-Ig, Clone: 3A7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

YAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66900-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:6,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

Immunocytochemistry (ICC/IF)

1:100

View 16 publications 16 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A7A9

Immunogen

YAP1 Fusion Protein Ag28194

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fibrotic Matrix Induces Mesenchymal Transformation of Epithelial Cells in Oral Submucous Fibrosis.

The American journal of pathology

Xu HQ,Guo ZX,Yan JF,Wang SY,Gao JL,Han XX,Qin WP,Lu WC,Gao CH,Zhu WW,Fu YT,Jiao K

Fri Sep 01 00:00:00 EDT 2023

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liang W,Zuo J,Liu M,Su Y,Guo B,Hou J,Xing Q,Peng Y,Fang L,Cao Y,Shan J,Sun R,Zhao J,Wang J

Sun Jan 01 00:00:00 EST 2023

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liang W,Zuo J,Liu M,Su Y,Guo B,Hou J,Xing Q,Peng Y,Fang L,Cao Y,Shan J,Sun R,Zhao J,Wang J

Sun Jan 01 00:00:00 EST 2023

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liang W,Zuo J,Liu M,Su Y,Guo B,Hou J,Xing Q,Peng Y,Fang L,Cao Y,Shan J,Sun R,Zhao J,Wang J

Sun Jan 01 00:00:00 EST 2023

The m⁶A methyltransferase METTL3 affects cell proliferation and migration by regulating YAP expression in Hirschsprung disease.

Pediatric surgery international

Huang Z,Luo C,Hou X,Yu D,Su Y,Li X,Luo Y,Liao G,Mu J,Wu K

Wed Feb 15 00:00:00 EST 2023

The m⁶A methyltransferase METTL3 affects cell proliferation and migration by regulating YAP expression in Hirschsprung disease.

Pediatric surgery international

Huang Z,Luo C,Hou X,Yu D,Su Y,Li X,Luo Y,Liao G,Mu J,Wu K

Wed Feb 15 00:00:00 EST 2023

Alteration in branching morphogenesis via YAP/TAZ in fibroblasts of fetal lungs in an LPS-induced inflammation model.

Molecular medicine (Cambridge, Mass.)

Ko HS,Laiman V,Tsao PN,Chen CM,Chuang HC

Mon Jan 30 00:00:00 EST 2023

Alteration in branching morphogenesis via YAP/TAZ in fibroblasts of fetal lungs in an LPS-induced inflammation model.

Molecular medicine (Cambridge, Mass.)

Ko HS,Laiman V,Tsao PN,Chen CM,Chuang HC

Mon Jan 30 00:00:00 EST 2023

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer.

Cancer communications (London, England)

Guo Y,Cui Y,Li Y,Jin X,Wang D,Lei M,Chen F,Liu Y,Xu J,Yao G,Zeng G,Chen X

Mon May 01 00:00:00 EDT 2023

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer.

Cancer communications (London, England)

Guo Y,Cui Y,Li Y,Jin X,Wang D,Lei M,Chen F,Liu Y,Xu J,Yao G,Zeng G,Chen X

Mon May 01 00:00:00 EDT 2023

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer.

Cancer communications (London, England)

Guo Y,Cui Y,Li Y,Jin X,Wang D,Lei M,Chen F,Liu Y,Xu J,Yao G,Zeng G,Chen X

Mon May 01 00:00:00 EDT 2023

Induction of Stem-Cell-Derived Cardiomyogenesis by Fibroblast Growth Factor 10 (FGF10) and Its Interplay with Cardiotrophin-1 (CT-1).

Biology

Khosravi F,Ahmadvand N,Wartenberg M,Sauer H

Wed Mar 30 00:00:00 EDT 2022

Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif.

Toxicology and applied pharmacology

Chen X,Wei X,Ma S,Xie H,Huang S,Yao M,Zhang L

Thu Dec 15 00:00:00 EST 2022

Toxicology and applied pharmacology

Chen X,Wei X,Ma S,Xie H,Huang S,Yao M,Zhang L

Thu Dec 15 00:00:00 EST 2022

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer.

Oncogene

Li M,Rao X,Cui Y,Zhang L,Li X,Wang B,Zheng Y,Teng L,Zhou T,Zhuo W

Tue Feb 01 00:00:00 EST 2022

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer.

Oncogene

Li M,Rao X,Cui Y,Zhang L,Li X,Wang B,Zheng Y,Teng L,Zhou T,Zhuo W

Tue Feb 01 00:00:00 EST 2022

N-Acetyl-l-tryptophan inhibits CCl₄-induced hepatic fibrogenesis via regulating TGF-β1/SMAD and Hippo/YAP1 signal.

Bioorganic chemistry

Ma T,Cheng H,Li T,Chen Y,Cai T,Bai J,Wu Z,Xia X,Liang T,Du Y,Fu W

Thu Sep 01 00:00:00 EDT 2022

N-Acetyl-l-tryptophan inhibits CCl₄-induced hepatic fibrogenesis via regulating TGF-β1/SMAD and Hippo/YAP1 signal.

Bioorganic chemistry

Ma T,Cheng H,Li T,Chen Y,Cai T,Bai J,Wu Z,Xia X,Liang T,Du Y,Fu W

Thu Sep 01 00:00:00 EDT 2022

Critical role of PAFR/YAP1 positive feedback loop in cardiac fibrosis.

Acta pharmacologica Sinica

Li TY,Su W,Li LL,Zhao XG,Yang N,Gai JX,Lv X,Zhang J,Huang MQ,Zhang Q,Ji WH,Song XY,Zhou YH,Li XL,Shan HL,Liang HH

Tue Nov 01 00:00:00 EDT 2022

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

Oncogene

Li Y,Yang J,Zhang Q,Xu S,Sun W,Ge S,Xu X,Jager MJ,Jia R,Zhang J,Fan X

Fri Jul 01 00:00:00 EDT 2022

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

Oncogene

Li Y,Yang J,Zhang Q,Xu S,Sun W,Ge S,Xu X,Jager MJ,Jia R,Zhang J,Fan X

Fri Jul 01 00:00:00 EDT 2022

Reduction of Emphysema Severity by Human Umbilical Cord-Derived Mesenchymal Stem Cells in Mice.

International journal of molecular sciences

Laiman V,Lee YL,Hou YW,Fang YT,Chen YY,Lo YC,Heriyanto DS,Lan SC,Chen CL,Chen XY,Lee KY,Chang JH,Chuang HC

Wed Aug 10 00:00:00 EDT 2022

Pan-cancer multi-omics analyses reveal crosstalk between the Hippo and immune signaling pathways in the tumor microenvironment.

FEBS letters

Wang C,Zhu T,Zhao Z,Chen B,Chen T,Dong Q,Liu M,Zhuang S,Yang F,Liu Y,Yang M,Gu Y,Liang H

Tue Feb 01 00:00:00 EST 2022

Pan-cancer multi-omics analyses reveal crosstalk between the Hippo and immune signaling pathways in the tumor microenvironment.

FEBS letters

Wang C,Zhu T,Zhao Z,Chen B,Chen T,Dong Q,Liu M,Zhuang S,Yang F,Liu Y,Yang M,Gu Y,Liang H

Tue Feb 01 00:00:00 EST 2022

Pan-cancer multi-omics analyses reveal crosstalk between the Hippo and immune signaling pathways in the tumor microenvironment.

FEBS letters

Wang C,Zhu T,Zhao Z,Chen B,Chen T,Dong Q,Liu M,Zhuang S,Yang F,Liu Y,Yang M,Gu Y,Liang H

Tue Feb 01 00:00:00 EST 2022

Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development.

Cellular and molecular gastroenterology and hepatology

Chen S,Wu H,Wang Z,Jia M,Guo J,Jin J,Li X,Meng D,Lin L,He AR,Zhou P,Zhi X

Mon Apr 04 00:00:00 EDT 2022

Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development.

Cellular and molecular gastroenterology and hepatology

Chen S,Wu H,Wang Z,Jia M,Guo J,Jin J,Li X,Meng D,Lin L,He AR,Zhou P,Zhi X

Mon Apr 04 00:00:00 EDT 2022

Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development.

Cellular and molecular gastroenterology and hepatology

Chen S,Wu H,Wang Z,Jia M,Guo J,Jin J,Li X,Meng D,Lin L,He AR,Zhou P,Zhi X

Mon Apr 04 00:00:00 EDT 2022

Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development.

Cellular and molecular gastroenterology and hepatology

Chen S,Wu H,Wang Z,Jia M,Guo J,Jin J,Li X,Meng D,Lin L,He AR,Zhou P,Zhi X

Mon Apr 04 00:00:00 EDT 2022

Fusobacterium nucleatum reduces METTL3-mediated m⁶A modification and contributes to colorectal cancer metastasis.

Nature communications

Chen S,Zhang L,Li M,Zhang Y,Sun M,Wang L,Lin J,Cui Y,Chen Q,Jin C,Li X,Wang B,Chen H,Zhou T,Wang L,Hsu CH,Zhuo W

Thu Mar 10 00:00:00 EST 2022

Fibrous Structure and Stiffness of Designer Protein Hydrogels Synergize to Regulate Endothelial Differentiation of Bone Marrow Mesenchymal Stem Cells.

Biomacromolecules

Tian KK,Huang SC,Xia XX,Qian ZG

Mon Apr 11 00:00:00 EDT 2022

Air pollution-regulated E-cadherin mediates contact inhibition of proliferation via the hippo signaling pathways in emphysema.

Chemico-biological interactions

Chang JH,Lee YL,Laiman V,Han CL,Jheng YT,Lee KY,Yeh CT,Kuo HP,Chung KF,Heriyanto DS,Hsiao TC,Wu SM,Ho SC,Chuang KJ,Chuang HC

Wed Jan 05 00:00:00 EST 2022

Air pollution-regulated E-cadherin mediates contact inhibition of proliferation via the hippo signaling pathways in emphysema.

Chemico-biological interactions

Chang JH,Lee YL,Laiman V,Han CL,Jheng YT,Lee KY,Yeh CT,Kuo HP,Chung KF,Heriyanto DS,Hsiao TC,Wu SM,Ho SC,Chuang KJ,Chuang HC

Wed Jan 05 00:00:00 EST 2022

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

Fri Jan 01 00:00:00 EST 2021

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

International journal of molecular medicine

Jia X,Wu B,Huang J,Fan L,Yang M,Xu W

Fri Jan 01 00:00:00 EST 2021

Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP.

Molecular and cellular biochemistry

Zhang Q,Li W,Zhu Y,Wang Q,Zhai C,Shi W,Feng W,Wang J,Yan X,Chai L,Chen Y,Li C,Liu P,Li M

Sun Aug 01 00:00:00 EDT 2021

Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP.

Molecular and cellular biochemistry

Zhang Q,Li W,Zhu Y,Wang Q,Zhai C,Shi W,Feng W,Wang J,Yan X,Chai L,Chen Y,Li C,Liu P,Li M

Sun Aug 01 00:00:00 EDT 2021

Luteolin Ameliorates Experimental Pulmonary Arterial Hypertension via Suppressing Hippo-YAP/PI3K/AKT Signaling Pathway.

Frontiers in pharmacology

Zuo W,Liu N,Zeng Y,Xiao Z,Wu K,Yang F,Li B,Song Q,Xiao Y,Liu Q

Tue May 04 00:00:00 EDT 2021

Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure.

Ecotoxicology and environmental safety

Yu FF,Zuo J,Fu X,Gao MH,Sun L,Yu SY,Li Z,Zhou GY,Ba Y

Wed Dec 01 00:00:00 EST 2021

ITGB1 enhances the Radioresistance of human Non-small Cell Lung Cancer Cells by modulating the DNA damage response and YAP1-induced Epithelial-mesenchymal Transition.

International journal of biological sciences

Li Y,Sun C,Tan Y,Zhang H,Li Y,Zou H

Mon Jan 03 00:00:00 EST 2022

Folic Acid Alleviates High Glucose and Fat-Induced Pyroptosis via Inhibition of the Hippo Signal Pathway on H9C2 Cells.

Frontiers in molecular biosciences

Hong L,Zha Y,Wang C,Qiao S,An J

Tue Oct 26 00:00:00 EDT 2021

CircATRNL1 promotes epithelial-mesenchymal transition in endometriosis by upregulating Yes-associated protein 1 in vitro.

Cell death & disease

Wang D,Luo Y,Wang G,Yang Q

Wed Jul 29 00:00:00 EDT 2020

CircATRNL1 promotes epithelial-mesenchymal transition in endometriosis by upregulating Yes-associated protein 1 in vitro.

Cell death & disease

Wang D,Luo Y,Wang G,Yang Q

Wed Jul 29 00:00:00 EDT 2020

Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.

Immunology letters

Gao P,Mu M,Chen Y,He J,Tao X,Song C

Tue Sep 01 00:00:00 EDT 2020

Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.

Journal of experimental & clinical cancer research : CR

Cao MX,Zhang WL,Yu XH,Wu JS,Qiao XW,Huang MC,Wang K,Wu JB,Tang YJ,Jiang J,Liang XH,Tang YL

Wed Jun 03 00:00:00 EDT 2020

Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.

Journal of experimental & clinical cancer research : CR

Cao MX,Zhang WL,Yu XH,Wu JS,Qiao XW,Huang MC,Wang K,Wu JB,Tang YJ,Jiang J,Liang XH,Tang YL

Wed Jun 03 00:00:00 EDT 2020

CLDN6 promotes tumor progression through the YAP1-snail1 axis in gastric cancer.

Cell death & disease

Yu S,Zhang Y,Li Q,Zhang Z,Zhao G,Xu J

Wed Dec 11 00:00:00 EST 2019

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

American journal of translational research

Bhandari A,Guan Y,Xia E,Huang Q,Chen Y

Thu Oct 01 00:00:00 EDT 2020

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.

Oncotarget

Zhang X,Fan Q,Li Y,Yang Z,Yang L,Zong Z,Wang B,Meng X,Li Q,Liu J,Li H

Tue May 02 00:00:00 EDT 2017

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.

Oncotarget

Zhang X,Fan Q,Li Y,Yang Z,Yang L,Zong Z,Wang B,Meng X,Li Q,Liu J,Li H

Tue May 02 00:00:00 EDT 2017

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.

Oncotarget

Zhang X,Fan Q,Li Y,Yang Z,Yang L,Zong Z,Wang B,Meng X,Li Q,Liu J,Li H

Tue May 02 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

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Gene Aliases: AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie

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UniProt ID: (Human) P46937, (Mouse) P46938

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Entrez Gene ID: (Human) 10413, (Rat) 363014, (Mouse) 22601

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Function(s)

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding chromatin binding transcription coactivator activity transcription corepressor activity protein binding protein C-terminus binding transcription regulatory region DNA binding proline-rich region binding transcription cofactor

Process(es)

cell morphogenesis vasculogenesis embryonic heart tube morphogenesis transcription initiation from RNA polymerase II promoter cellular response to DNA damage stimulus cell proliferation positive regulation of cell proliferation regulation of keratinocyte proliferation keratinocyte differentiation negative regulation of epithelial cell differentiation notochord development somatic stem cell population maintenance hippo signaling positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth paraxial mesoderm development lateral mesoderm development regulation of neurogenesis contact inhibition bud elongation involved in lung branching lung epithelial cell differentiation cellular response to retinoic acid cellular response to gamma radiation regulation of stem cell proliferation regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of canonical Wnt signaling pathway positive regulation of stem cell population maintenance negative regulation of nucleic acid-templated transcription negative regulation of stem cell differentiation negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter DNA damage induced protein phosphorylation regulation of cell proliferation regulation of canonical Wnt signaling pathway

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YAP1 Monoclonal Antibody (3A7A9)

YAP1 Antibody (66900-1-IG) in ICC/IF

Product Details

66900-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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