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Invitrogen
YAP1 Recombinant Rabbit Monoclonal Antibody (SU33-06)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 15
YAP1 Antibody (MA5-32117) in ICC/IF
Immunofluorescence analysis of YAP1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with YAP1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-23117) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 48... View More
Product Details
MA5-32117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SU33-06
Immunogen
Synthetic peptide within Human YAP1 aa 421-470
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809409
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yorkie homolog
Gene Aliases: COB1; YAP; YAP1; YAP2; YAP65; YKI
UniProt ID: (Human) P46937
Entrez Gene ID: (Human) 10413
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein binding
protein C-terminus binding
transcription regulatory region DNA binding
proline-rich region binding
transcription cofactor
cell morphogenesis
vasculogenesis
embryonic heart tube morphogenesis
transcription initiation from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell proliferation
positive regulation of cell proliferation
regulation of keratinocyte proliferation
keratinocyte differentiation
negative regulation of epithelial cell differentiation
notochord development
somatic stem cell population maintenance
hippo signaling
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
regulation of neurogenesis
contact inhibition
bud elongation involved in lung branching
lung epithelial cell differentiation
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
positive regulation of stem cell population maintenance
negative regulation of nucleic acid-templated transcription
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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