Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
YAP1 (R331W Mutant) Antibody (PA5-117450) in WB
Western blot analysis of YAP1 (R331W Mutant) in Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg). Samples were separated with 10% SDS-PAGE and incubated in YAP1 (R331W Mutant) polyclonal antibody (Product # PA5-117450) using a dilution of 1:5,000, followed by HRP-conjugated anti-rabbit IgG.
Product Details
PA5-117450
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human YAP1 (R331W Mutant). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2902080
Product Specific Information
Centrifuge briefly prior to opening.
YAP1 is an oncogene and the YAP1 R331W missense mutation from the original family may be associated with lung adenocarcinoma.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yorkie homolog
Gene Aliases: COB1; YAP; YAP1; YAP2; YAP65; YKI
UniProt ID: (Human) P46937
Entrez Gene ID: (Human) 10413
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein binding
protein C-terminus binding
transcription regulatory region DNA binding
proline-rich region binding
transcription cofactor
cell morphogenesis
vasculogenesis
embryonic heart tube morphogenesis
transcription initiation from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell proliferation
positive regulation of cell proliferation
regulation of keratinocyte proliferation
keratinocyte differentiation
negative regulation of epithelial cell differentiation
notochord development
somatic stem cell population maintenance
hippo signaling
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
regulation of neurogenesis
contact inhibition
bud elongation involved in lung branching
lung epithelial cell differentiation
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
positive regulation of stem cell population maintenance
negative regulation of nucleic acid-templated transcription
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support