Proteintech

YY1 Monoclonal Antibody (2E11C5)

YY1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

YY1 Antibody (66281-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 66281-1-IG) (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

YY1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

YY1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66281-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

ChIP assay (ChIP)

View 5 publications 5 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2E11C5

Immunogen

YY1 Fusion Protein Ag17732 (1-414 aa encoded by BC037308)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MASGDTLYI ATDGSEMPAE IVELHEIEVE TIPVETIETT VVGEEEEEDD DDEDGGGGDH GGGGGHGHAG HHHHHHHHHH HPPMIALQPL VTDDPTQVHH HQEVILVQTR EEVVGGDDSD GLRAEDGFED QILIPVPAPA GGDDDYIEQT LVTVAAAGKS GGGGSSSSGG GRVKKGGGKK SGKKSYLSGG AGAAGGGGAD PGNKKWEQKQ VQIKTLEGEF SVTMWSSDEK KDIDHETVVE EQIIGENSPP DYSEYMTGKK LPPGGIPGID LSDPKQLAEF ARMKPRKIKE DDAPRTIACP HKGCTKMFRD NSAMRKHLHT HGPRVHVCAE CGKAFVESSK LKRHQLVHTG EKPFQCTFEG CGKRFSLDFN LRTHVRIHTG DRPYVCPFDG CNKKFAQSTN LKSHILTHAK AKNNQ (1-414 aa encoded by BC037308)

Target Information

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression.

Journal of experimental & clinical cancer research : CR

Wei W,Zhang ZY,Shi B,Cai Y,Zhang HS,Sun CL,Fei YF,Zhong W,Zhang S,Wang C,He B,Jiang GM,Wang H

Tue Jun 20 00:00:00 EDT 2023

LINC01608 activated by YY1 facilitate hepatocellular carcinoma progression by modulating the EGFR/ERK axis.

Liver international : official journal of the International Association for the Study of the Liver

Han M,Liu F,Li X,Zhang H,Pan Y,Liu Y,Zhu H,Liang H,Chen X,Liao Z,Zhang Z,Zhang B

Wed Feb 01 00:00:00 EST 2023

LINC01608 activated by YY1 facilitate hepatocellular carcinoma progression by modulating the EGFR/ERK axis.

Liver international : official journal of the International Association for the Study of the Liver

Han M,Liu F,Li X,Zhang H,Pan Y,Liu Y,Zhu H,Liang H,Chen X,Liao Z,Zhang Z,Zhang B

Wed Feb 01 00:00:00 EST 2023

Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis.

Chemosphere

Hu H,Li L,Zhang H,Zhang Y,Liu Q,Chen M,Ning J,Pang Y,Hu W,Niu Y,Zhang R

Wed Feb 01 00:00:00 EST 2023

Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis.

Chemosphere

Hu H,Li L,Zhang H,Zhang Y,Liu Q,Chen M,Ning J,Pang Y,Hu W,Niu Y,Zhang R

Wed Feb 01 00:00:00 EST 2023

YAP1 synergize with YY1 transcriptional co-repress DUSP1 to induce osimertinib resistant by activating the EGFR/MAPK pathway and abrogating autophagy in non-small cell lung cancer.

International journal of biological sciences

Ning Y,Zheng H,Yang Y,Zang H,Wang W,Zhan Y,Wang H,Luo J,Wen Q,Peng J,Xiang J,Fan S

Wed May 24 00:00:00 EDT 2023

YY1 lactylation in microglia promotes angiogenesis through transcription activation-mediated upregulation of FGF2.

Genome biology

Wang X,Fan W,Li N,Ma Y,Yao M,Wang G,He S,Li W,Tan J,Lu Q,Hou S

Fri Apr 21 00:00:00 EDT 2023

Atractylodes lancea volatile oils target ADAR2-miR-181a-5p signaling to mesenchymal stem cell chondrogenic differentiation.

Anatomical record (Hoboken, N.J. : 2007)

Ye S,Si W,Qin W,Yang L,Luo Z,Li Z,Xie Y,Pan H,Li X,Huang Z,Zhu M,Chen D

Fri Dec 01 00:00:00 EST 2023

Silencing of microRNA-106b-5p prevents doxorubicin-mediated cardiotoxicity through modulation of the PR55α/YY1/sST2 signaling axis.

Molecular therapy. Nucleic acids

Lax A,Soler F,Fernandez Del Palacio MJ,Pascual-Oliver S,Ballester MR,Fuster JJ,Pascual-Figal D,Asensio-Lopez MDC

Tue Jun 13 00:00:00 EDT 2023

Long non‑coding RNA BLACAT2/miR‑378a‑3p/YY1 feedback loop promotes the proliferation, migration and invasion of uterine corpus endometrial carcinoma.

Oncology reports

Zhang C,Wang R,Li M,Yang Q

Mon May 01 00:00:00 EDT 2023

A T_reg-specific long noncoding RNA maintains immune-metabolic homeostasis in aging liver.

Nature aging

Ding C,Yu Z,Sefik E,Zhou J,Kaffe E,Wang G,Li B,Flavell RA,Hu W,Ye Y,Li HB

Sat Jul 01 00:00:00 EDT 2023

A T_reg-specific long noncoding RNA maintains immune-metabolic homeostasis in aging liver.

Nature aging

Ding C,Yu Z,Sefik E,Zhou J,Kaffe E,Wang G,Li B,Flavell RA,Hu W,Ye Y,Li HB

Sat Jul 01 00:00:00 EDT 2023

RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression.

Medical oncology (Northwood, London, England)

Zhang YL,Cao JL,Zhang Y,Liao L,Deng L,Yang SY,Hu SY,Ning Y,Zhang FL,Li DQ

Tue Feb 01 00:00:00 EST 2022

YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway.

Journal of experimental & clinical cancer research : CR

Li W,Ye K,Li X,Liu X,Peng M,Chen F,Xiong W,Wang Y,Zhu L

Wed Aug 17 00:00:00 EDT 2022

YY1 promotes pancreatic cancer cell proliferation by enhancing mitochondrial respiration.

Cancer cell international

Li B,Wang J,Liao J,Wu M,Yuan X,Fang H,Shen L,Jiang M

Mon Sep 19 00:00:00 EDT 2022

YY1 promotes pancreatic cancer cell proliferation by enhancing mitochondrial respiration.

Cancer cell international

Li B,Wang J,Liao J,Wu M,Yuan X,Fang H,Shen L,Jiang M

Mon Sep 19 00:00:00 EDT 2022

Stabilization but No Functional Influence of HIF-1α Expression in the Intestinal Epithelium during Salmonella Typhimurium Infection.

Infection and immunity

Robrahn L,Dupont A,Jumpertz S,Zhang K,Holland CH,Guillaume J,Rappold S,Roth J,Cerovic V,Saez-Rodriguez J,Hornef MW,Cramer T

Thu Feb 17 00:00:00 EST 2022

Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development.

International journal of molecular sciences

Dong Y,Wu X,Peng X,Yang L,Tan B,Zhao H,Zheng E,Hong L,Cai G,Wu Z,Li Z

Wed Nov 23 00:00:00 EST 2022

Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development.

International journal of molecular sciences

Dong Y,Wu X,Peng X,Yang L,Tan B,Zhao H,Zheng E,Hong L,Cai G,Wu Z,Li Z

Wed Nov 23 00:00:00 EST 2022

Transcription factor YY1 contributes to human melanoma cell growth through modulating the p53 signalling pathway.

Experimental dermatology

Zhou S,Li P,Qin L,Huang S,Dang N

Sat Oct 01 00:00:00 EDT 2022

Transcription factor YY1 contributes to human melanoma cell growth through modulating the p53 signalling pathway.

Experimental dermatology

Zhou S,Li P,Qin L,Huang S,Dang N

Sat Oct 01 00:00:00 EDT 2022

Transcription factor YY1 contributes to human melanoma cell growth through modulating the p53 signalling pathway.

Experimental dermatology

Zhou S,Li P,Qin L,Huang S,Dang N

Sat Oct 01 00:00:00 EDT 2022

Long noncoding RNA LINC01435 impedes diabetic wound healing by facilitating YY1-mediated HDAC8 expression.

iScience

Fu W,Liang D,Wu X,Chen H,Hong X,Wang J,Zhu T,Zeng T,Lin W,Chen S,Yan L,Ren M

Fri Apr 15 00:00:00 EDT 2022

Long noncoding RNA LINC01435 impedes diabetic wound healing by facilitating YY1-mediated HDAC8 expression.

iScience

Fu W,Liang D,Wu X,Chen H,Hong X,Wang J,Zhu T,Zeng T,Lin W,Chen S,Yan L,Ren M

Fri Apr 15 00:00:00 EDT 2022

Long noncoding RNA LINC01435 impedes diabetic wound healing by facilitating YY1-mediated HDAC8 expression.

iScience

Fu W,Liang D,Wu X,Chen H,Hong X,Wang J,Zhu T,Zeng T,Lin W,Chen S,Yan L,Ren M

Fri Apr 15 00:00:00 EDT 2022

Angiotensin II-induced muscle atrophy via PPARγ suppression is mediated by miR-29b.

Molecular therapy. Nucleic acids

Li J,Yang T,Sha Z,Tang H,Hua X,Wang L,Wang Z,Gao Z,Sluijter JPG,Rowe GC,Das S,Yang L,Xiao J

Fri Mar 05 00:00:00 EST 2021

Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment.

Environmental toxicology

Huang X,Zhou W,Zhang Y

Fri Jan 01 00:00:00 EST 2021

Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression.

Ecotoxicology and environmental safety

Liu C,Qu J,Wu M,Huang X,Li L

Wed Dec 01 00:00:00 EST 2021

Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression.

Ecotoxicology and environmental safety

Liu C,Qu J,Wu M,Huang X,Li L

Wed Dec 01 00:00:00 EST 2021

YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling.

American journal of cancer research

Mei C,Jiang X,Gu Y,Wu X,Ma W,Chen X,Wang G,Yao Y,Liu Y,Zhang Z,Yuan Y

Tue Jun 08 00:00:00 EDT 2021

Prenatal dexamethasone exposure induces nonalcoholic fatty liver disease in male rat offspring via the miR-122/YY1/ACE2-MAS1 pathway.

Biochemical pharmacology

Liu H,He B,Hu W,Liu K,Dai Y,Zhang D,Wang H

Mon Mar 01 00:00:00 EST 2021

Acinetobacter baumannii up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1.

Virulence

An Z,Ding W

Wed Dec 01 00:00:00 EST 2021

Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis.

Molecular brain

Dong X,Kwan KM

Thu Jul 23 00:00:00 EDT 2020

MiR-302b as a Combinatorial Therapeutic Approach to Improve Cisplatin Chemotherapy Efficacy in Human Triple-Negative Breast Cancer.

Cancers

Cataldo A,Romero-Cordoba S,Plantamura I,Cosentino G,Hidalgo-Miranda A,Tagliabue E,Iorio MV

Wed Aug 12 00:00:00 EDT 2020

FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.

Journal of cellular and molecular medicine

Yang W,Feng B,Meng Y,Wang J,Geng B,Cui Q,Zhang H,Yang Y,Yang J

Wed May 01 00:00:00 EDT 2019

The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.

Nature communications

Tang W,Zhou W,Xiang L,Wu X,Zhang P,Wang J,Liu G,Zhang W,Peng Y,Huang X,Cai J,Bai Y,Bai L,Zhu W,Gu H,Xiong J,Ye C,Li A,Liu S,Wang J

Fri Feb 08 00:00:00 EST 2019

The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.

Nature communications

Tang W,Zhou W,Xiang L,Wu X,Zhang P,Wang J,Liu G,Zhang W,Peng Y,Huang X,Cai J,Bai Y,Bai L,Zhu W,Gu H,Xiong J,Ye C,Li A,Liu S,Wang J

Fri Feb 08 00:00:00 EST 2019

CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p.

Molecular cancer

Rong D,Lu C,Zhang B,Fu K,Zhao S,Tang W,Cao H

Mon Feb 18 00:00:00 EST 2019

hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells.

The Prostate

Yang T,An Z,Zhang C,Wang Z,Wang X,Liu Y,Du E,Liu R,Zhang Z,Xu Y

Thu Aug 01 00:00:00 EDT 2019

hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells.

The Prostate

Yang T,An Z,Zhang C,Wang Z,Wang X,Liu Y,Du E,Liu R,Zhang Z,Xu Y

Thu Aug 01 00:00:00 EDT 2019

Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription.

Cell

Xiao R,Chen JY,Liang Z,Luo D,Chen G,Lu ZJ,Chen Y,Zhou B,Li H,Du X,Yang Y,San M,Wei X,Liu W,Lécuyer E,Graveley BR,Yeo GW,Burge CB,Zhang MQ,Zhou Y,Fu XD

Thu Jun 27 00:00:00 EDT 2019

CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7.

Cell death & disease

Zeng K,Chen X,Xu M,Liu X,Hu X,Xu T,Sun H,Pan Y,He B,Wang S

Sun Apr 01 00:00:00 EDT 2018

CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7.

Cell death & disease

Zeng K,Chen X,Xu M,Liu X,Hu X,Xu T,Sun H,Pan Y,He B,Wang S

Sun Apr 01 00:00:00 EDT 2018

miR-29b contributes to multiple types of muscle atrophy.

Nature communications

Li J,Chan MC,Yu Y,Bei Y,Chen P,Zhou Q,Cheng L,Chen L,Ziegler O,Rowe GC,Das S,Xiao J

Thu May 25 00:00:00 EDT 2017

miR-29b contributes to multiple types of muscle atrophy.

Nature communications

Li J,Chan MC,Yu Y,Bei Y,Chen P,Zhou Q,Cheng L,Chen L,Ziegler O,Rowe GC,Das S,Xiao J

Thu May 25 00:00:00 EDT 2017

MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1.

Stem cell research

Jin M,Wu Y,Wang Y,Yu D,Yang M,Yang F,Feng C,Chen T

Thu Sep 01 00:00:00 EDT 2016

MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1.

Stem cell research

Jin M,Wu Y,Wang Y,Yu D,Yang M,Yang F,Feng C,Chen T

Thu Sep 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Delta transcription factor; INO80 complex subunit S; NF-E1; Transcriptional repressor protein YY1; UCR-motif DNA-binding protein; UCRBP transcription factor; Yin and yang 1; Yin and Yang 1 protein; Yin Yang 1; YY-1

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Gene Aliases: AW488674; DELTA; INO80S; NF-E1; NMP-1; NMP1; UCRBP; YIN-YANG-1; YY1

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UniProt ID: (Human) P25490, (Mouse) Q00899

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Entrez Gene ID: (Human) 7528, (Rat) 24919, (Mouse) 22632

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Function(s)

four-way junction DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity RNA binding protein binding zinc ion binding transcription regulatory region DNA binding SMAD binding histone deacetylase binding sequence-specific DNA binding metal ion binding nucleic acid binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter double-strand break repair via homologous recombination transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular response to DNA damage stimulus spermatogenesis response to UV-C negative regulation of gene expression cell differentiation cellular response to UV regulation of transcription, DNA-templated transcription from RNA polymerase II promoter RNA localization anterior/posterior pattern specification positive regulation of gene expression response to prostaglandin F positive regulation of transcription from RNA polymerase II promoter camera-type eye morphogenesis chromosome organization cellular response to interleukin-1 DNA repair DNA recombination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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YY1 Monoclonal Antibody (2E11C5)

YY1 Antibody (66281-1-IG) in ICC/IF

Product Details

66281-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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