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Invitrogen
YY1 Recombinant Rabbit Monoclonal Antibody (SY29-01)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
YY1 Antibody (MA5-32052) in ICC/IF
Immunofluorescence analysis of YY1 was performed using DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with YY1 Mouse Monoclonal Antibody (Product # MA5-32052) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for ... View More
Product Details
MA5-32052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SY29-01
Immunogen
Synthetic peptide within Human YY1 aa 255-304
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809346
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Delta transcription factor; INO80 complex subunit S; NF-E1; Transcriptional repressor protein YY1; UCR-motif DNA-binding protein; UCRBP transcription factor; Yin and yang 1; Yin and Yang 1 protein; Yin Yang 1; YY-1
Gene Aliases: AW488674; DELTA; INO80S; NF-E1; NMP-1; NMP1; UCRBP; YIN-YANG-1; YY1
UniProt ID: (Human) P25490, (Mouse) Q00899
Entrez Gene ID: (Human) 7528, (Rat) 24919, (Mouse) 22632
four-way junction DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
enhancer sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
RNA binding
protein binding
zinc ion binding
transcription regulatory region DNA binding
SMAD binding
histone deacetylase binding
sequence-specific DNA binding
metal ion binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
spermatogenesis
response to UV-C
negative regulation of gene expression
cell differentiation
cellular response to UV
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
RNA localization
anterior/posterior pattern specification
positive regulation of gene expression
response to prostaglandin F
positive regulation of transcription from RNA polymerase II promoter
camera-type eye morphogenesis
chromosome organization
cellular response to interleukin-1
DNA repair
DNA recombination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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