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ZBP89 Antibody (A303-116A) in WB
Detection of human and mouse ZBP89 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ZBP89 antibody A303-116A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. ZBP89 was also immunoprecipitated by rabbit anti-ZBP89 antibody A303-117A, which recognizes a downstream epitope. Detection: Chemilumine... View More
Product Details
A303-116A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 375 and 425 of Human Zinc Finger DNA-Binding Protein 89.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan
Target Information
ZNF148 is involved in transcriptional regulation. ZNF148 represses the transcription of a number of genes including gastrin, stromelysin and enolase. ZNF148 binds to the G-rich box in the enhancer region of these genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta enolase repressor factor 1; CACCC box-binding protein; CLL-associated antigen KW-10; G-rich box-binding protein; Transcription factor BFCOL1; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; Zinc finger protein 148
Gene Aliases: 2210405J08Rik; AI480666; AW045217; BERF-1; BFCOL1; HT-BETA; pHZ-52; ZBP-89; ZBP89; ZFP148; ZNF148
UniProt ID: (Human) Q9UQR1, (Mouse) Q61624
Entrez Gene ID: (Human) 7707, (Mouse) 22661
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
sequence-specific DNA binding
metal ion binding
transcription regulatory region sequence-specific DNA binding
nucleic acid binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein complex assembly
cellular defense response
gamete generation
negative regulation of gene expression
substantia nigra development
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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