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ZEB1 Antibody (MA5-44395) in WB
Western Blot of ZEB1 in whole cell lysate (50 µg) from C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells. Samples were treated with NETN lysis buffer, incubated with ZEB1 monoclonal antibody (Product # MA5-44396) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 30 seconds) Lower Panel: Rabbit anti-COPB2.
Product Details
MA5-44395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR102H
Immunogen
Peptide representing a region between residue 1074 and C-terminus (residue 1124) of human zinc finger E-box-binding homeobox 1
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
purified
Storage buffer
BBS, pH 7.8-8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
2-8°C
Shipping conditions
Wet ice
RRID
AB_2926525
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; zinc finger homeodomain enhancer-binding protein
Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha
UniProt ID: (Human) P37275, (Mouse) Q64318
Entrez Gene ID: (Human) 6935, (Mouse) 21417
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
protein binding
transcription factor binding
zinc ion binding
E-box binding
nucleic acid binding
DNA binding
double-stranded DNA binding
sequence-specific DNA binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
pattern specification process
central nervous system development
cell proliferation
negative regulation of cell proliferation
regulation of mesenchymal cell proliferation
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of epithelial cell differentiation
regulation of T cell differentiation in thymus
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic camera-type eye morphogenesis
embryonic skeletal system morphogenesis
semicircular canal morphogenesis
regulation of smooth muscle cell differentiation
cartilage development
cellular response to amino acid stimulus
cochlea morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
nervous system development
cell differentiation
animal organ development
embryonic morphogenesis
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