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ZEB2 Antibody (PA5-78302) in ICC/IF
ZEB2 Polyclonal Antibody detects ZEB2 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB2 stained by ZEB2 Polyclonal Antibody (Product # PA5-78302) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000.
Product Details
PA5-78302
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZEB2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736815
Product Specific Information
Positive Control: MEF, A375, Neuro 2A, C8D30, Raw264.7, C2C12, rat brain
Predicted Reactivity: Xenopus laevis (87%), Chicken (93%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; Zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b
Gene Aliases: 9130203F04Rik; D130016B08Rik; HRIHFB2411; HSPC082; KIAA0569; SIP-1; SIP1; SMADIP1; ZEB2; ZFHX1B; ZFX1B; Zfxh1b
UniProt ID: (Human) A0JP09, (Mouse) Q9R0G7
Entrez Gene ID: (Human) 9839, (Mouse) 24136
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
phosphatase regulator activity
metal ion binding
R-SMAD binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
neural crest cell migration
somitogenesis
neural tube closure
transcription from RNA polymerase II promoter
nervous system development
corpus callosum morphogenesis
hippocampus development
cell proliferation in forebrain
corticospinal tract morphogenesis
positive regulation of Wnt signaling pathway
positive regulation of JUN kinase activity
positive regulation of melanocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of melanin biosynthetic process
developmental pigmentation
collateral sprouting
positive regulation of axonogenesis
mammillary axonal complex development
melanocyte migration
positive regulation of lens fiber cell differentiation
regulation of melanosome organization
transcription, DNA-templated
regulation of transcription, DNA-templated
central nervous system development
embryonic morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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