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ZFHX3 Antibody (PA5-119848) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human stomach carcinoma tissue using ZFHX3 Polyclonal Antibody (Product # PA5-119848). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ZFHX3 antibody at a ... View More
Product Details
PA5-119848
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human ATBF1 aa 1,800-2,000
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913420
Product Specific Information
Positive Control: Rat esophagus tissue, human colon tissue, human breast carcinoma tissue, human stomach carcinoma tissue.
Subcellular Location: Nucleus, Cytoplasm.
Western Blot: 1:500 for endogenous lysates (predicted); 1:5,000 for recombinant protein.
Target Information
Transcriptional repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. Down-regulates the MUC5AC promoter in gastric cancer. Miura Y., J. Biol. Chem. 270:26840-26848(1995). Morinaga T., Mol. Cell. Biol. 11:6041-6049(1991). Martin J., Nature 432:988-994(2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-fetoprotein enhancer binding protein; Alpha-fetoprotein enhancer-binding protein; AT motif-binding factor 1; AT-binding transcription factor 1; ZFH-3; zinc finger homeobox 3; Zinc finger homeobox protein 3; Zinc finger homeodomain protein 3
Gene Aliases: ATBF1; ATBT; C16orf47; RGD1560268; ZFHX3; ZNF927
UniProt ID: (Human) Q15911
Entrez Gene ID: (Human) 463, (Rat) 307829
core promoter sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
zinc ion binding
enzyme binding
molecular_function
sequence-specific DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
brain development
muscle organ development
circadian regulation of gene expression
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
response to transforming growth factor beta
regulation of locomotor rhythm
negative regulation of myoblast differentiation
positive regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
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