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ZFYVE21 Antibody (CF505622) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE21 (RC203337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFYV. Positive lysates LY411387 (100 µg) and LC411387 (20 µg) can be purchased separately from OriGene.
Product Details
CF505622
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4A2
Immunogen
Full length human recombinant protein of human ZFYVE21 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FYVE domain containing 21; HCV p7-transactivated protein 1; ZF21; Zinc finger FYVE domain-containing protein 21; zinc finger, FYVE domain containing 21
Gene Aliases: HCVP7TP1; ZF21; ZFYVE21
UniProt ID: (Human) Q9BQ24
Entrez Gene ID: (Human) 79038
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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