Invitrogen

ZO-1 Polyclonal Antibody

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

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ZO-1 Antibody (18-7430) in ICC/IF

Immunofluorescent analysis of ZO-1 (red) in Caco-2 cells. The cells were blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature, stained with a ZO-1 Rabbit polyclonal antibody (Product # 18-7430) at a concentration of 1:500 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:1000 for 30 minutes at room temperature (red). Nuclei (blue) were stained wit... View More

ZO-1 Antibody in Immunohistochemistry (IHC)

ZO-1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

18-7430

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250-1:1,000

View 8 publications 8 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence from the N-terminal region of human ZO-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533048

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methodological study of directed differentiation of pluripotent stem cells into corneal endothelial cells.

Annals of translational medicine

Jia L,Diao Y,Fang Y,Yang K,Wang L,Huang Y

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Fri Apr 01 00:00:00 EDT 2022

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Annals of translational medicine

Zhang H,Su B,Jiao L,Xu ZH,Zhang CJ,Nie J,Gao ML,Zhang YV,Jin ZB

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Annals of translational medicine

Zhang H,Su B,Jiao L,Xu ZH,Zhang CJ,Nie J,Gao ML,Zhang YV,Jin ZB

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Annals of translational medicine

Zhang H,Su B,Jiao L,Xu ZH,Zhang CJ,Nie J,Gao ML,Zhang YV,Jin ZB

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Development of a Scalable Coculture System for Gut Anaerobes and Human Colon Epithelium.

Gastroenterology

Sasaki N,Miyamoto K,Maslowski KM,Ohno H,Kanai T,Sato T

18-7430 was used in Immunocytochemistry to establish iHACS, a system that provides optimal growth environments for human colonic epithelium and gut anaerobes.

Wed Jul 01 00:00:00 EDT 2020

A C-terminal peptide from type I interferon protects the retina in a mouse model of autoimmune uveitis.

PloS one

Ahmed CM,Ildefonso CJ,Johnson HM,Lewin AS

18-7430 was used in Immunohistochemistry to propose that IFNα-C peptide exhibits anti-inflammatory properties and protects autoimmune uveitis mice against damage to retinal structure and function.

Wed Apr 22 00:00:00 EDT 2020

A C-terminal peptide from type I interferon protects the retina in a mouse model of autoimmune uveitis.

PloS one

Ahmed CM,Ildefonso CJ,Johnson HM,Lewin AS

18-7430 was used in Immunohistochemistry to propose that IFNα-C peptide exhibits anti-inflammatory properties and protects autoimmune uveitis mice against damage to retinal structure and function.

Wed Apr 22 00:00:00 EDT 2020

Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis.

International journal of cell biology

Devocelle A,Lecru L,François H,Desterke C,Gallerne C,Eid P,Estelle O,Azzarone B,Giron-Michel J

18-7430 was used in Immunocytochemistry-immunoflourescence to analyse whether human chronic inflammatory nephropathies evolving towards fibrosis could be also characterized by a weak intrarenal IL-15 expression and if IL-15 could inhibit epithelial-mesenchymal transition (EMT) and excess matrix deposition in human renal proximal tubular epithelial cells (RPTEC).

Wed Sep 30 00:00:00 EDT 2020

Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage.

Stem cells translational medicine

Choudhary P,Booth H,Gutteridge A,Surmacz B,Louca I,Steer J,Kerby J,Whiting PJ

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Wed Feb 01 00:00:00 EST 2017

Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage.

Stem cells translational medicine

Choudhary P,Booth H,Gutteridge A,Surmacz B,Louca I,Steer J,Kerby J,Whiting PJ

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Wed Feb 01 00:00:00 EST 2017

Elovanoids are novel cell-specific lipid mediators necessary for neuroprotective signaling for photoreceptor cell integrity.

Scientific reports

Jun B,Mukherjee PK,Asatryan A,Kautzmann MA,Heap J,Gordon WC,Bhattacharjee S,Yang R,Petasis NA,Bazan NG

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunofluorescence

Thu Jul 13 00:00:00 EDT 2017

Targeting the cAMP and Transforming Growth Factor-β Pathway Increases Proliferation to Promote Re-Epithelialization of Human Stem Cell-Derived Retinal Pigment Epithelium.

Stem cells translational medicine

Choudhary P,Gutteridge A,Impey E,Storer RI,Owen RM,Whiting PJ,Bictash M,Benn CL

187430 was used in immunocytochemistry to optimize retinal pigment epithelium yield after differentiation

Fri Jul 01 00:00:00 EDT 2016

Targeting the cAMP and Transforming Growth Factor-β Pathway Increases Proliferation to Promote Re-Epithelialization of Human Stem Cell-Derived Retinal Pigment Epithelium.

Stem cells translational medicine

Choudhary P,Gutteridge A,Impey E,Storer RI,Owen RM,Whiting PJ,Bictash M,Benn CL

187430 was used in immunocytochemistry to optimize retinal pigment epithelium yield after differentiation

Fri Jul 01 00:00:00 EDT 2016

A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells.

PloS one

Choudhary P,Dodsworth BT,Sidders B,Gutteridge A,Michaelides C,Duckworth JK,Whiting PJ,Benn CL

18-7430 was used in immunocytochemistry to determine the molecular mechanisms that affect the epithelial phenotype.

Tue Mar 29 00:00:00 EDT 2016

Lung epithelial branching program antagonizes alveolar differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Chang DR,Martinez Alanis D,Miller RK,Ji H,Akiyama H,McCrea PD,Chen J

18-7430 was used in Immunohistochemistry to show that an epithelial branching morphogenesis program antagonises alveolar differentiation in the mouse lung.

Tue Nov 05 00:00:00 EST 2013

Integrin Beta 1 suppresses multilayering of a simple epithelium.

PloS one

Chen J,Krasnow MA

18-7430 was used in immunohistochemistry - frozen section to study the conversion of simple epithelium into multilayer epithelium following ablation of integrin beta in a murine model.

Tue Jul 23 00:00:00 EDT 2013

Differential expression of claudin tight junction proteins in the human cortical nephron.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Kirk A,Campbell S,Bass P,Mason J,Collins J

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunohistochemistry

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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Gene Aliases: TJP1; ZO-1; ZO1

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UniProt ID: (Human) Q07157

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Entrez Gene ID: (Human) 7082

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Function(s)

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion tight junction

Process(es)

blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-1 Polyclonal Antibody

ZO-1 Antibody (18-7430) in ICC/IF

Product Details

18-7430

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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