Product References
Bacterial meningitis in the early postnatal mouse studied at single-cell resolution.
eLife
Wang J,Rattner A,Nathans J
40-2200 was used in Immunohistochemistry to imply that EC responses to infection are largely driven by the intrinsic EC response to LPS.
Thu Jun 15 00:00:00 EDT 2023
Maternal e-cigarette use can disrupt postnatal blood-brain barrier (BBB) integrity and deteriorates motor, learning and memory function: influence of sex and age.
Fluids and barriers of the CNS
Archie SR,Sifat AE,Zhang Y,Villalba H,Sharma S,Nozohouri S,Abbruscato TJ
40-2200 was used in Western Blotting to suggest that prenatal e-cig exposure induces long-term neurovascular changes of neonates by disrupting postnatal BBB integrity and worsening behavioral outcomes.
Fri Mar 10 00:00:00 EST 2023
Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration.
Stem cell research & therapy
Dushime H,Moreno SG,Linard C,Adrait A,Couté Y,Peltzer J,Messiaen S,Torres C,Bensemmane L,Lewandowski D,Romeo PH,Petit V,Gault N
40-2200 was used in Immunohistochemistry to indicate that a single injection of a small quantity of WJ-Muse may be a new and easy therapeutic strategy for treating lethal GIS.
Fri Aug 11 00:00:00 EDT 2023
Hepatocyte apical bulkheads provide a mechanical means to oppose bile pressure.
The Journal of cell biology
Bebelman MP,Bovyn MJ,Mayer CM,Delpierre J,Naumann R,Martins NP,Honigmann A,Kalaidzidis Y,Haas PA,Zerial M
40-2200 was used in Immunocytochemistry-immunofluorescence to demonstrate that apical bulkheads are load-bearing mechanical elements that could protect the BC network against elevated pressure.
Mon Apr 03 00:00:00 EDT 2023
Aberrant Akt2 signaling in the RPE may contribute to retinal fibrosis process in diabetic retinopathy.
Cell death discovery
Daley R,Maddipatla V,Ghosh S,Chowdhury O,Hose S,Zigler JS,Sinha D,Liu H
40-2200 was used in Western Blot to find that in Akt2 cKO mice diabetes-induced increase of fibrosis markers, including collagen IV, Connective tissue growth factor (CTGF), fibronectin, and alpha-SMA was attenuated.
Thu Jul 13 00:00:00 EDT 2023
Effects of Volatile Anesthetics versus Ketamine on Blood-Brain Barrier Permeability via Lipid-Mediated Alterations of Endothelial Cell Membranes.
The Journal of pharmacology and experimental therapeutics
Noorani B,Chowdhury EA,Alqahtani F,Ahn Y,Nozohouri E,Zoubi S,Patel D,Wood L,Huang J,Siddique MB,Al-Ahmad A,Mehvar R,Bickel U
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to support membrane permeabilization rather than loosening of intercellular tight junctions as an underlying mechanism for increased permeability of the endothelial cell monolayers and the BBB in vivo.
Mon May 01 00:00:00 EDT 2023
Morphological and Functional Effects of Ultrasound on Blood-Brain Barrier Transitory Opening: An In Vitro Study on Rat Brain Endothelial Cells.
Cells
Branca JJV,Boninsegna M,Morucci G,Carrino D,Nicoletti C,Paternostro F,Gulisano M,Bocchi L,Pacini A
40-2200 was used in Immunocytochemistry-immunofluorescence to carry out an in depth in vitro molecular and morphological study on the effects of US treatment on the BBB.
Tue Jan 03 00:00:00 EST 2023
NLRP3 Exacerbate NETosis-Associated Neuroinflammation in an LPS-Induced Inflamed Brain.
Immune network
Byun DJ,Lee J,Yu JW,Hyun YM
40-2200 was used in Immunohistochemistry to provide a clue that NLRP3 would be a potential therapeutic target to alleviate neuroinflammation.
Thu Jun 01 00:00:00 EDT 2023
Therapeutic effect of human ApoA-I-Milano variant in aged transgenic mouse model of Alzheimer's disease.
British journal of pharmacology
Solé M,Marazuela P,Castellote L,Bonaterra-Pastra A,Giménez-Llort L,Hernández-Guillamon M
40-2200 was used in Western Blotting to show the potential therapeutic applicability of a safe and non-invasive treatment based on peripheral administration of hrApoA-I-M in Alzheimer's disease.
Tue Aug 01 00:00:00 EDT 2023
Natural History of Glaucoma Progression in the DBA/2J Model: Early Contribution of Müller Cell Gliosis.
Cells
Amato R,Cammalleri M,Melecchi A,Bagnoli P,Porciatti V
40-2200 was used in Western Blotting to indicate that Müller cell activation occurs well before IOP elevation, with probable inflammatory consequences, and represents an early event in the glaucomatous process.
Thu Apr 27 00:00:00 EDT 2023
Switching from high-fat diet to normal diet ameliorate BTB integrity and improve fertility potential in obese male mice.
Scientific reports
Zhang W,Tian Z,Qi X,Chen P,Yang Q,Guan Q,Ye J,Yu C
40-2200 was used in Western Blotting to explore the effects of switching from a high-fat diet (HFD) to a normal diet (ND) on the fertility potential of obese male mice and its related mechanisms.
Tue Aug 29 00:00:00 EDT 2023
Lung infection by Pseudomonas aeruginosa induces neuroinflammation and blood-brain barrier dysfunction in mice.
Journal of neuroinflammation
Villalba N,Ma Y,Gahan SA,Joly-Amado A,Spence S,Yang X,Nash KR,Yuan SY
40-2200 was used in Immunocytochemistry-immunofluorescence to examine the effects of lung infection causing systemic and neuroinflammation as a potential mechanism contributing to blood-brain barrier (BBB) leakage and behavioral impairment.
Sat May 27 00:00:00 EDT 2023
ZFP750 affects the cutaneous barrier through regulating lipid metabolism.
Science advances
Butera A,Agostini M,Cassandri M,De Nicola F,Fanciulli M,D'Ambrosio L,Falasca L,Nardacci R,Wang L,Piacentini M,Knight RA,Jia W,Sun Q,Shi Y,Wang Y,Candi E,Melino G
40-2200 was used in Immunohistochemistry to identify the transcription factor ZFP750 as a master regulator epidermal homeostasis through lipid biosynthesis and thus contributing to our understanding of the pathogenesis of several human skin diseases.
Fri Apr 28 00:00:00 EDT 2023
The Role of Neutrophil Extracellular Traps in Early Microthrombosis and Brain Injury After Subarachnoid Hemorrhage in Mice.
Translational stroke research
Hao X,Zeng Z,Liang L,Feng Z,Li W,Xiong B,Guo P,Zhang Q,Chen Y,Feng H,Chen Z
40-2200 was used in Western Blotting to find that DNase I treatment significantly improved the restoration of early cortical perfusion after SAH.
Sun Oct 01 00:00:00 EDT 2023
Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures.
Cell reports
Cossec JC,Traboulsi T,Sart S,Loe-Mie Y,Guthmann M,Hendriks IA,Theurillat I,Nielsen ML,Torres-Padilla ME,Baroud CN,Dejean A
40-2200 was used in Immunocytochemistry-immunofluorescence to show that mouse embryonic stem cells (ESCs) solely exposed to chemical inhibition of SUMOylation generate embryo-like structures comprising anterior neural and trunk-associated regions.
Tue Apr 25 00:00:00 EDT 2023
Soluble CX3CL1-expressing retinal pigment epithelium cells protect rod photoreceptors in a mouse model of retinitis pigmentosa.
Stem cell research & therapy
Jong ED,Hacibekiroglu S,Guo L,Sawula E,Li B,Li C,Ho MT,Shoichet MS,Wallace VA,Nagy A
40-2200 was used in Immunocytochemistry-immunofluorescence to reveal the potential of a combined cell and gene therapy for the treatment of RP, but also the possibility of using hRPE cells to deliver therapeutic biologics in situ to treat diseases over long-term.
Mon Aug 21 00:00:00 EDT 2023
Inhibition of Abl Kinase by Imatinib Can Rescue the Compromised Barrier Function of 22q11.2DS Patient-iPSC-Derived Blood-Brain Barriers.
Cells
Li Y,Sun Z,Zhu H,Sun Y,Shteyman DB,Markx S,Leong KW,Xu B,Fu BM
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to indicate that the 22q11.2DS iBBB is at least partially caused by the haploinsufficiency of CRKL, which can be rescued by imatinib via its effects on the Abl/CRKL signaling pathway.
Fri Jan 27 00:00:00 EST 2023
Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier.
Neuron
Ayloo S,Lazo CG,Sun S,Zhang W,Cui B,Gu C
40-2200 was used in Immunohistochemistry to show that vitronectin, an extracellular matrix protein secreted by central nervous system pericytes, as a regulator of blood-central nervous system barrier function via interactions with its integrin receptor, α5, in endothelial cells.
Wed May 18 00:00:00 EDT 2022
Circular RNA circ-FoxO3 attenuates blood-brain barrier damage by inducing autophagy during ischemia/reperfusion.
Molecular therapy : the journal of the American Society of Gene Therapy
Yang Z,Huang C,Wen X,Liu W,Huang X,Li Y,Zang J,Weng Z,Lu D,Tsang CK,Li K,Xu A
40-2200 was used in Western Blot, Immunohistochemistry to demonstrate that the circular RNA of FoxO3 promotes autophagy via mTORC1 inhibition to attenuate BBB collapse under ischemia/reperfusion injury.
Wed Mar 02 00:00:00 EST 2022
Circular RNA circ-FoxO3 attenuates blood-brain barrier damage by inducing autophagy during ischemia/reperfusion.
Molecular therapy : the journal of the American Society of Gene Therapy
Yang Z,Huang C,Wen X,Liu W,Huang X,Li Y,Zang J,Weng Z,Lu D,Tsang CK,Li K,Xu A
40-2200 was used in Western Blot, Immunohistochemistry to demonstrate that the circular RNA of FoxO3 promotes autophagy via mTORC1 inhibition to attenuate BBB collapse under ischemia/reperfusion injury.
Wed Mar 02 00:00:00 EST 2022
DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
Journal of biomedical science
Chou HC,Cheng CM,Yang CH,Lin TY,Liu YW,Tan TH,Chen YR
40-2200 was used in Western Blotting to reveal that DUSP3 is an important TJ regulatory protein and its decrease may be involved in progression of epithelial cancers.
Wed Jun 15 00:00:00 EDT 2022
Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells.
PLoS biology
Zhu Y,Chew KY,Wu M,Karawita AC,McCallum G,Steele LE,Yamamoto A,Labzin LI,Yarlagadda T,Khromykh AA,Wang X,Sng JDJ,Stocks CJ,Xia Y,Kollmann TR,Martino D,Joensuu M,Meunier FA,Balistreri G,Bielefeldt-Ohmann H,Bowen AC,Kicic A,Sly PD,Spann KM,Short KR
40-2200 was used in Immunocytochemistry-immunoflourescence to show that the nasal epithelium of children supports lower infection and replication of ancestral SARS-CoV-2, although this may be changing as the virus evolves.
Mon Aug 01 00:00:00 EDT 2022
A gastruloid model of the interaction between embryonic and extra-embryonic cell types.
Journal of tissue engineering
Bérenger-Currias NM,Mircea M,Adegeest E,van den Berg PR,Feliksik M,Hochane M,Idema T,Tans SJ,Semrau S
40-2200 was used in Immunohistochemistry-immunofluorescence to establish XEGs to explore heterotypic cellular interactions and their developmental consequences in vitro.
Sat Jul 16 00:00:00 EDT 2022
Effect of heated tobacco products and traditional cigarettes on pulmonary toxicity and SARS-CoV-2-induced lung injury.
Toxicology
Tsou HH,Wang PH,Ting TH,Ping YH,Liu TY,Cheng HW,Wang HT
40-2200 was used in Immunohistochemistry-immunofluorescence to suggest that HTPE induced similar lung pathogenesis relevant to COPD and SARS-CoV-2-induced lung injury caused by CSE.
Thu Sep 01 00:00:00 EDT 2022
Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors.
International journal of molecular sciences
Yue Q,Zhou X,Zhang Z,Hoi MPM
40-2200 was used in Immunocytochemistry, Western Blot to show that murine sAβO42 was moderately toxic to an endothelial and astrocyte co-culture.
Mon Feb 07 00:00:00 EST 2022
Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors.
International journal of molecular sciences
Yue Q,Zhou X,Zhang Z,Hoi MPM
40-2200 was used in Immunocytochemistry, Western Blot to show that murine sAβO42 was moderately toxic to an endothelial and astrocyte co-culture.
Mon Feb 07 00:00:00 EST 2022
Cell-Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis.
Cells
Gomes de Almeida P,Rifes P,Martins-Jesus AP,Pinheiro GG,Andrade RP,Thorsteinsdóttir S
40-2200 was used in Immunohistochemistry to suggest that the fibronectin-integrin-ROCK-NM II axis regulates segmentation clock dynamics and dictates the spatio-temporal localization of somitic clefts.
Wed Jun 22 00:00:00 EDT 2022
Vitamin B3, nicotinamide, enhances mitochondrial metabolism to promote differentiation of the retinal pigment epithelium.
The Journal of biological chemistry
Hazim RA,Paniagua AE,Tang L,Yang K,Kim KKO,Stiles L,Divakaruni AS,Williams DS
40-2200 was used in Immunocytochemistry to investigate the ways in which mitochondrial metabolism affect retinal pigment epithelium cellular functions are not clear.
Thu Sep 01 00:00:00 EDT 2022
Chronic exposure to synthetic food colorant Allura Red AC promotes susceptibility to experimental colitis via intestinal serotonin in mice.
Nature communications
Kwon YH,Banskota S,Wang H,Rossi L,Grondin JA,Syed SA,Yousefi Y,Schertzer JD,Morrison KM,Wade MG,Holloway AC,Surette MG,Steinberg GR,Khan WI
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to reveal that chronic long-term exposure to AR promotes experimental colitis via colonic 5-HT in gut microbiota-dependent and -independent pathway in mice.
Tue Dec 20 00:00:00 EST 2022
Acute Hyperglycemia Exacerbates Hemorrhagic Transformation after Embolic Stroke and Reperfusion with tPA: A Possible Role of TXNIP-NLRP3 Inflammasome.
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association
Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T
40-2200 was used in Western Blotting to suggest a potential role for TXNIP in the occurrence of HT in hyperglycemic conditions following eMCAO.
Tue Feb 01 00:00:00 EST 2022
A microengineered Brain-Chip to model neuroinflammation in humans.
iScience
Pediaditakis I,Kodella KR,Manatakis DV,Le CY,Barthakur S,Sorets A,Gravanis A,Ewart L,Rubin LL,Manolakos ES,Hinojosa CD,Karalis K
40-2200 was used in Immunocytochemistry to present a human organotypic microphysiological system (MPS) that includes endothelial-like cells, pericytes, glia, and cortical neurons and maintains brain and blood-brain barrier permeability at in vivo relevant levels.
Fri Aug 19 00:00:00 EDT 2022
Neuroprotective effects of an in vitro BBB permeable phenoxythiophene sulfonamide small molecule in glutamate-induced oxidative injury.
Experimental and therapeutic medicine
Pokharel S,Gliyazova NS,Dandepally SR,Williams AL,Ibeanu GC
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to indicate that the phenoxythiophene sulfonamide compound B355227 has potent antioxidant and neuroprotective attributes in glutamate-induced neuronal cell death.
Sat Jan 01 00:00:00 EST 2022
Microcarrier-Based Culture of Human Pluripotent Stem-Cell-Derived Retinal Pigmented Epithelium.
Bioengineering (Basel, Switzerland)
Faynus MA,Bailey JK,Pennington BO,Katsura M,Proctor DA,Yeh AK,Menon S,Choi DG,Lebkowski JS,Johnson LV,Clegg DO
40-2200 was used in Immunocytochemistry to investigate treatment options for dry age-related macular degeneration via organoids.
Mon Jul 04 00:00:00 EDT 2022
RGMa Participates in the Blood-Brain Barrier Dysfunction Through BMP/BMPR/YAP Signaling in Multiple Sclerosis.
Frontiers in immunology
Zhang L,Tang S,Ma Y,Liu J,Monnier P,Li H,Zhang R,Yu G,Zhang M,Li Y,Feng J,Qin X
40-2200 was used in Western Blotting to show that RGMa causes BBB dysfunction in endothelial cells via BMP2/BMPR II/YAP, resulting in BBB integrity disruption in MS and that it could be a novel therapeutic target for BBB permeability in MS.
Wed Jun 08 00:00:00 EDT 2022
Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in Chronic Obstructive Pulmonary Disease.
Microbiology spectrum
Osan J,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M
40-2200 was used in Immunohistochemistry to determine whether SARS-CoV-2's cellular tropism plays a critical role in severe pathophysiology in the lung.
Wed Aug 31 00:00:00 EDT 2022
TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response.
Cell reports
Meena AS,Shukla PK,Bell B,Giorgianni F,Caires R,Fernández-Peña C,Beranova S,Aihara E,Montrose MH,Chaib M,Makowski L,Neeli I,Radic MZ,Vásquez V,Jaggar JH,Cordero-Morales JF,Rao R
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to identify TRPV6, a Ca2+-permeable channel, as responsible for alcohol-induced elevation of intracellular Ca2+, intestinal barrier dysfunction, and systemic inflammation.
Tue Jun 14 00:00:00 EDT 2022
A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress.
Methods in molecular biology (Clifton, N.J.)
Molins Gutiérrez G,Martorell J,Salazar-Martin AG,Balcells M
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to design and synthesize a three-dimensional scaffold that recapitulates the main hallmarks of the BBB extracellular matrix and serves as a platform to co-culture human brain microvascular endothelial cells and human cortical astrocytes.
Fri Jun 24 00:00:00 EDT 2022
Bioengineered intestinal tubules as a tool to test intestinal biological efficacy of lettuce species.
NPJ science of food
Jochems PGM,Heming B,Lapin D,Moonen NEL,Van den Ackerveken G,Masereeuw R
40-2200 was used in Immunocytochemistry-immunofluorescence to assess the epithelial barrier integrity, cell viability, cell attachment, brush border enzyme activity, and immune markers.
Tue Dec 13 00:00:00 EST 2022
High-Dose Acetaminophen Alters the Integrity of the Blood-Brain Barrier and Leads to Increased CNS Uptake of Codeine in Rats.
Pharmaceutics
Yang J,Betterton RD,Williams EI,Stanton JA,Reddell ES,Ogbonnaya CE,Dorn E,Davis TP,Lochhead JJ,Ronaldson PT
40-2200 was used in Immunohistochemistry-immunofluorescence to show that BBB integrity is altered following a single high APAP dose, results that are relevant to patients abusing or misusing APAP and/or APAP/opioid combination products.
Wed Apr 27 00:00:00 EDT 2022
A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecups.
Scientific reports
Landowski M,Bhute VJ,Takimoto T,Grindel S,Shahi PK,Pattnaik BR,Ikeda S,Ikeda A
40-2200 was used in Immunohistochemistry to conclude that Tmem135 mutant mice can serve as a good model for the role of dysregulated lipid metabolism in AMD.
Fri Jan 14 00:00:00 EST 2022
Effects of intermittent hypoxia with thrombin in an in vitro model of human brain endothelial cells and their impact on PAR-1/PAR-3 cleavage.
Scientific reports
Zolotoff C,Puech C,Roche F,Perek N
40-2200 was used in ELISA to investigate if thrombin and protease activated receptors represent novel attractive targets to prevent blood-brain barrier opening in obstructive sleep apnea/hypopnea.
Tue Jul 19 00:00:00 EDT 2022
HMGN3 represses transcription of epithelial regulators to promote migration of cholangiocarcinoma in a SNAI2-dependent manner.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Sorin S,Kubota S,Hamidi S,Yokomizo-Nakano T,Vaeteewoottacharn K,Wongkham S,Waraasawapati S,Pairojkul C,Bai J,Morii M,Sheng G,Sawanyawisuth K,Sashida G
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that the overexpression of HMGN3 represses the transcription of and blocks the polarities of epithelial regulators in CCA cells in a manner that is dependent on the SNAI2 gene and HDACs.
Fri Jul 01 00:00:00 EDT 2022
A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis.
Science advances
Gerstmann K,Kindbeiter K,Telley L,Bozon M,Reynaud F,Théoulle E,Charoy C,Jabaudon D,Moret F,Castellani V
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to discover that two different complexes, Sema3B/Nrp2 and Sema3F/Nrp1, exert dual effects on apical endfeet dynamics, nuclei positioning, and RGC progeny.
Wed Nov 16 00:00:00 EST 2022
Blood-Brain Barrier Disruption Mediated by FFA1 Receptor-Evidence Using Miniscope.
International journal of molecular sciences
Lindenau KL,Barr JL,Higgins CR,Sporici KT,Brailoiu E,Brailoiu GC
40-2200 was used in Immunocytochemistry-immunofluorescence to demonstrate that FFA1 receptor activation reduced RBMVEC barrier function and produced a transient increase in BBB permeability.
Fri Feb 18 00:00:00 EST 2022
Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner.
eLife
Richards M,Nwadozi E,Pal S,Martinsson P,Kaakinen M,Gloger M,Sjöberg E,Koltowska K,Betsholtz C,Eklund L,Nordling S,Claesson-Welsh L
40-2200 was used in Western Blotting to identify structural and functional in vivo characteristics of the endothelial cell barrier.
Thu Jul 21 00:00:00 EDT 2022
N-myc downstream regulated gene 1 (ndrg1) functions as a molecular switch for cellular adaptation to hypoxia.
eLife
Park JS,Gabel AM,Kassir P,Kang L,Chowdhary PK,Osei-Ntansah A,Tran ND,Viswanathan S,Canales B,Ding P,Lee YS,Brewster R
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to support a model whereby Ndrg1a is essential for hypoxia adaptation and functions downstream of lactate signaling to induce NKA degradation, a process known to conserve cellular energy.
Mon Oct 10 00:00:00 EDT 2022
Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing.
International journal of biological macromolecules
Rofaani E,Huang B,Liang F,Peng J,Chen Y
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to generate airway epithelium by using such an artificial basement membrane (ABM) and human-induced pluripotent stem cells (hiPSCs).
Fri Apr 15 00:00:00 EDT 2022
Type 2 Diabetes Mellitus Makes Corneal Endothelial Cells Vulnerable to Ultraviolet A-Induced Oxidative Damage Via Decreased DJ-1/Nrf2/NQO1 Pathway.
Investigative ophthalmology & visual science
Zhang X,Qiu J,Huang F,Shan K,Zhang C
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that an increase of ROS, downregulation of DJ-1/Nrf2/NQO1 expressions, and decrease of Nrf2 nuclear translocation could result in that T2DM makes CECs more vulnerable to oxidative damage.
Tue Nov 01 00:00:00 EDT 2022
Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury.
Frontiers in neurology
Zhou R,Li J,Wang R,Chen Z,Zhou F
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that moderate systemic TH does not have a protective effect on the disrupted BSCB in early SCI.
Sat Dec 03 00:00:00 EST 2022
Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury.
Frontiers in neurology
Zhou R,Li J,Wang R,Chen Z,Zhou F
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that moderate systemic TH does not have a protective effect on the disrupted BSCB in early SCI.
Sat Dec 03 00:00:00 EST 2022
Engineered human organ-specific urethra as a functional substitute.
Scientific reports
Caneparo C,Chabaud S,Fradette J,Bolduc S
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that substitutes produced with VF and at least 10% of DF showed sufficient mechanical resistance to withstand surgeon manipulation and high functionality, which may improve long-term patients' quality of life, representing a great future alternative to current treatments.
Fri Dec 09 00:00:00 EST 2022
Hypoxia induces purinergic receptor signaling to disrupt endothelial barrier function.
Frontiers in physiology
Raghavan S,Brishti MA,Collier DM,Leo MD
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that P2Y1R signaling in endothelial cells leads to the downregulation of endothelial barrier function.
Sat Dec 10 00:00:00 EST 2022
Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Wang W,Su L,Wang Y,Li C,Ji F,Jiao J
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to provide mechanistic insights into how endothelial UCP2 regulates the neurogenic-to-gliogenic fate switch in the developing neocortex.
Wed Jun 01 00:00:00 EDT 2022
Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Wang W,Su L,Wang Y,Li C,Ji F,Jiao J
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to provide mechanistic insights into how endothelial UCP2 regulates the neurogenic-to-gliogenic fate switch in the developing neocortex.
Wed Jun 01 00:00:00 EDT 2022
TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling.
Cell reports
Sivakumar S,Qi S,Cheng N,Sathe AA,Kanchwala M,Kumar A,Evers BM,Xing C,Yu H
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs).
Tue Feb 15 00:00:00 EST 2022
TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling.
Cell reports
Sivakumar S,Qi S,Cheng N,Sathe AA,Kanchwala M,Kumar A,Evers BM,Xing C,Yu H
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs).
Tue Feb 15 00:00:00 EST 2022
The Protection of Zinc against Acute Cadmium Exposure: A Morphological and Molecular Study on a BBB In Vitro Model.
Cells
Branca JJV,Carrino D,Paternostro F,Morucci G,Fiorillo C,Nicoletti C,Gulisano M,Ghelardini C,Di Cesare Mannelli L,Becatti M,Pacini A
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to demonstrate that Zn protection from the SOD activity leads to ZO-1 downregulation and in blood-brain barrier damage.
Sun May 15 00:00:00 EDT 2022
A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles.
Journal of extracellular vesicles
Nguyen VVT,Ye S,Gkouzioti V,van Wolferen ME,Yengej FY,Melkert D,Siti S,de Jong B,Besseling PJ,Spee B,van der Laan LJW,Horland R,Verhaar MC,van Balkom BWM
40-2200 was used in Immunohistochemistry to develop a multi-organ-on-a-chip (MOC) model that combines human kidney and liver organoids connected by microfluidic channels to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived small extracellular vesicles (MSC-sEVs).
Tue Nov 01 00:00:00 EDT 2022
Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Maurizi E,Martella DA,Schiroli D,Merra A,Mustfa SA,Pellegrini G,Macaluso C,Chiappini C
40-2200 was used in Immunohistochemistry-immunofluorescence to indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.
Tue Nov 01 00:00:00 EDT 2022
The interplay between SARS-CoV-2 infected airway epithelium and immune cells modulates regulatory/inflammatory signals.
iScience
Bordoni V,Matusali G,Mariotti D,Antonioli M,Cimini E,Sacchi A,Tartaglia E,Casetti R,Grassi G,Notari S,Castilletti C,Fimia GM,Capobianchi MR,Ippolito G,Agrati C
40-2200 was used in Immunocytochemistry-immunoflourescence to investigate the cell-cell communication mechanisms necessary to develop novel therapeutic strategies aimed to promote an effective immune response.
Fri Feb 18 00:00:00 EST 2022
Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.
Fluids and barriers of the CNS
Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ
40-2200 was used in Western Blotting to suggest that JUUL exposure could negatively impact the cerebrovascular system, although to a lesser extent than TS exposure.
Fri Sep 09 00:00:00 EDT 2022
Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function.
Evidence-based complementary and alternative medicine : eCAM
Zhou Y,Zhang Z,Sun Y,Zhou D,Zhao Y,Li J,Guo W
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that LTP can effectively reduce the degree of brain damage in AICH rats, and the mechanism may be that it can play a neuroprotective role by regulating the function of the intestinal mucosal barrier.
Tue Jan 03 00:00:00 EST 2023
Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration.
Nature cell biology
Pérez-González C,Ceada G,Greco F,Matejčić M,Gómez-González M,Castro N,Menendez A,Kale S,Krndija D,Clark AG,Gannavarapu VR,Álvarez-Varela A,Roca-Cusachs P,Batlle E,Vignjevic DM,Arroyo M,Trepat X
40-2200 was used in Immunohistochemistry to provide high-resolution dynamic maps of cell-ECM and cell-cell forces exerted by the intestinal epithelium.
Thu Jul 01 00:00:00 EDT 2021
Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads.
The Journal of cell biology
Belicova L,Repnik U,Delpierre J,Gralinska E,Seifert S,Valenzuela JI,Morales-Navarrete HA,Franke C,Räägel H,Shcherbinina E,Prikazchikova T,Koteliansky V,Vingron M,Kalaidzidis YL,Zatsepin T,Zerial M
40-2200 was used in Immunocytochemistry to study the process of lumenogenesis in mouse hepatoblasts.
Mon Oct 04 00:00:00 EDT 2021
AIM2 deletion enhances blood-brain barrier integrity in experimental ischemic stroke.
CNS neuroscience & therapeutics
Xu SY,Bian HJ,Shu S,Xia SN,Gu Y,Zhang MJ,Xu Y,Cao X
40-2200 was used in Western Blot to explore the effects of AIM2 on the BBB integrity in mice with middle cerebral artery occlusion induced injury and in oxygen-glucose deprivation/reoxygenation induced human brain microvascular endothelial cells.
Fri Oct 01 00:00:00 EDT 2021
Cytochrome P450 expression, induction and activity in human induced pluripotent stem cell-derived intestinal organoids and comparison with primary human intestinal epithelial cells and Caco-2 cells.
Archives of toxicology
Janssen AWF,Duivenvoorde LPM,Rijkers D,Nijssen R,Peijnenburg AACM,van der Zande M,Louisse J
40-2200 was used in Immunocytochemistry to study chemical-induced changes in CYP expression and activity.
Mon Mar 01 00:00:00 EST 2021
Human Amniotic Epithelial Stem Cell-Derived Retinal Pigment Epithelium Cells Repair Retinal Degeneration.
Frontiers in cell and developmental biology
Li J,Qiu C,Wei Y,Yuan W,Liu J,Cui W,Zhou J,Qiu C,Guo L,Huang L,Ge Z,Yu L
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to propose that subretinal transplantation of hAESCs-RPE could be an optional therapeutic strategy for retinal degeneration diseases.
Sat Oct 16 00:00:00 EDT 2021
Direct Current Stimulation Disrupts Endothelial Glycocalyx and Tight Junctions of the Blood-Brain Barrier in vitro.
Frontiers in cell and developmental biology
Xia Y,Li Y,Khalid W,Bikson M,Fu BM
40-2200 was used in Western Blot to elucidate the structural and signaling mechanisms by which direct current stimulation modulates the BBB permeability.
Tue Aug 01 00:00:00 EDT 2023
Direct Current Stimulation Disrupts Endothelial Glycocalyx and Tight Junctions of the Blood-Brain Barrier in vitro.
Frontiers in cell and developmental biology
Xia Y,Li Y,Khalid W,Bikson M,Fu BM
40-2200 was used in Western Blot to elucidate the structural and signaling mechanisms by which direct current stimulation modulates the BBB permeability.
Tue Aug 01 00:00:00 EDT 2023
The Impact of Particulate Matter (PM2.5) on Human Retinal Development in hESC-Derived Retinal Organoids.
Frontiers in cell and developmental biology
Zeng Y,Li M,Zou T,Chen X,Li Q,Li Y,Ge L,Chen S,Xu H
40-2200 was used in Immunohistochemistry-immunofluorescence to analyze the influences and the mechanisms of PM2.5 on the developing retina.
Wed Mar 03 00:00:00 EST 2021
Food-Derived High Arginine Peptides Promote Spermatogenesis Recovery in Busulfan Treated Mice.
Frontiers in cell and developmental biology
Liu W,Zhang L,Gao A,Khawar MB,Gao F,Li W
40-2200 was used in Immunohistochemistry to conclude that high-arginine peptides might be used as a medication or therapeutic component of a diet prescription to improve the fertility of some oligoasthenospermia patients.
Sat Jan 08 00:00:00 EST 2022
Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function.
Experimental and therapeutic medicine
Liu MM,Zhou J,Ji D,Yang J,Huang YP,Wang Q
40-2200 was used in Western Blot to investigate the effects of diammonium glycyrrhizinate lipid ligand (DGLL) treatment on acute lung injury (ALI) and pulmonary edema induced by lipopolysaccharide (LPS) in Sprague-Dawley rats.
Thu Apr 01 00:00:00 EDT 2021
Steroid hormones and human choriogonadotropin influence the distribution of alpha6-integrin and desmoplakin 1 in gland-like endometrial epithelial spheroids.
Histochemistry and cell biology
Buck VU,Kohlen MT,Sternberg AK,Rösing B,Neulen J,Leube RE,Classen-Linke I
40-2200 was used in Immunohistochemistry to demonstrate that the 3D endometrial Ishikawa cell culture might be suited as an experimental model system to prove the effect of hormonal changes like those occurring during the window of implantation.
Sat May 01 00:00:00 EDT 2021
Role of miRNA-671-5p in Mediating Wnt/β-Catenin-Triggered Podocyte Injury.
Frontiers in pharmacology
Wang C,Liu J,Zhang X,Chen Q,Bai X,Hong X,Zhou L,Liu Y
40-2200 was used in Immunohistochemistry, Western Blot to test the hypothesis that β-catenin may down-regulate Wilms tumor 1 protein by regulating miRNAs in glomerular podocytes.
Fri Feb 04 00:00:00 EST 2022
Role of miRNA-671-5p in Mediating Wnt/β-Catenin-Triggered Podocyte Injury.
Frontiers in pharmacology
Wang C,Liu J,Zhang X,Chen Q,Bai X,Hong X,Zhou L,Liu Y
40-2200 was used in Immunohistochemistry, Western Blot to test the hypothesis that β-catenin may down-regulate Wilms tumor 1 protein by regulating miRNAs in glomerular podocytes.
Fri Feb 04 00:00:00 EST 2022
Functional model of rat thyroid follicles cultured in Matrigel.
Endocrine connections
Khoruzhenko A,Miot F,Massart C,Van Sande J,Dumont JE,Beauwens R,Boom A
40-2200 was used in Immunohistochemistry to develop a method of cultivation of functionally active rat thyroid follicles in Matrigel under three-dimensional conditions.
Mon May 24 00:00:00 EDT 2021
Lactobacillus casei and Epidermal Growth Factor Prevent Osmotic Stress-Induced Tight Junction Disruption in Caco-2 Cell Monolayers.
Cells
Samak G,Rao R,Rao R
40-2200 was used in Immunocytochemistry-immunoflourescence to suggest that L. casei and EGF ameliorate osmotic stress-induced disruption of apical junctional complexes and barrier dysfunction in the intestinal epithelium by distinct signaling mechanisms.
Sat Dec 18 00:00:00 EST 2021
Ethanol-induced lymphatic endothelial cell permeability via MAP-kinase regulation.
American journal of physiology. Cell physiology
Herrera M,Molina P,Souza-Smith FM
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to hypothesize that ethanol induces lymphatic endothelial cell (LEC) permeability via disruption of LEC TJ through MAPK activation.
Thu Jul 01 00:00:00 EDT 2021
A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications.
Molecular therapy. Methods & clinical development
Munis AM,Hyde SC,Gill DR
40-2200 was used in Immunocytochemistry to show human surfactant air-liquid interface cultures can be effective tools for studying human alveolar epithelium.
Fri Mar 12 00:00:00 EST 2021
miR-200c-3p Regulates Epitelial-to-Mesenchymal Transition in Epicardial Mesothelial Cells by Targeting Epicardial Follistatin-Related Protein 1.
International journal of molecular sciences
Pontemezzo E,Foglio E,Vernucci E,Magenta A,D'Agostino M,Sileno S,Astanina E,Bussolino F,Pellegrini L,Germani A,Russo MA,Limana F
40-2200 was used in Immunocytochemistry to investigate the role of miRNAs as regulators of epithelial to mesenchymal transition (EMT) in heart development and their potential targets.
Fri May 07 00:00:00 EDT 2021
Molecular Mechanism of Stimulation of Na-K-ATPase by Leukotriene D4 in Intestinal Epithelial Cells.
International journal of molecular sciences
Nepal N,Arthur S,Butts MR,Singh S,Palaniappan B,Sundaram U
40-2200 was used in Immunocytochemistry to investigate the way in which Leukotriene D4 (LTD4) may regulate Na-K-ATPase in an in vitro model of rat intestinal epithelial cells (IEC-18).
Thu Jul 15 00:00:00 EDT 2021
Mechanism of Na-K-ATPase Inhibition by PGE2 in Intestinal Epithelial Cells.
Cells
Nepal N,Arthur S,Haynes J,Palaniappan B,Sundaram U
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to determine the effect of PGE2 on Na-K-ATPase in villus cells and its mechanism of action.
Mon Mar 29 00:00:00 EDT 2021
Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo.
Scientific reports
Pennington BO,Bailey JK,Faynus MA,Hinman C,Hee MN,Ritts R,Nadar V,Zhu D,Mitra D,Martinez-Camarillo JC,Lin TC,Thomas BB,Hinton DR,Humayun MS,Lebkowski J,Johnson LV,Clegg DO
40-2200 was used in Immunocytochemistry to develop a cryopreservation method for human embryonic stem cell-derived retinal pigmented epithelial cells.
Thu Mar 18 00:00:00 EDT 2021
In vitro Measurement of Membrane Attack Complex in RPE Cells.
Bio-protocol
Mulfaul K,Doyle SL
40-2200 was used in Immunohistochemistry to measure membrane attack complex formation in RPE cell cultures.
Sat Feb 20 00:00:00 EST 2021
SARS-CoV-2-triggered mast cell rapid degranulation induces alveolar epithelial inflammation and lung injury.
Signal transduction and targeted therapy
Wu ML,Liu FL,Sun J,Li X,He XY,Zheng HY,Zhou YH,Yan Q,Chen L,Yu GY,Chang J,Jin X,Zhao J,Chen XW,Zheng YT,Wang JH
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot, Flow Cytometry to reveal ed an inductive role of Atg5-dependent autophagy as well as a previously unrecognized autophagy-independent inhibitory function of Becn1 in iCM reprogramming.
Fri Dec 17 00:00:00 EST 2021
Endothelial LRP1 protects against neurodegeneration by blocking cyclophilin A.
The Journal of experimental medicine
Nikolakopoulou AM,Wang Y,Ma Q,Sagare AP,Montagne A,Huuskonen MT,Rege SV,Kisler K,Dai Z,Körbelin J,Herz J,Zhao Z,Zlokovic BV
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to investigate lipoprotein receptor-related protein 1 roles in the blood-brain barrier in angiogenesis and Alzheimer's disease.
Mon Apr 05 00:00:00 EDT 2021
Modeling alpha-synuclein pathology in a human brain-chip to assess blood-brain barrier disruption.
Nature communications
Pediaditakis I,Kodella KR,Manatakis DV,Le CY,Hinojosa CD,Tien-Street W,Manolakos ES,Vekrellis K,Hamilton GA,Ewart L,Rubin LL,Karalis K
40-2200 was used in Immunohistochemistry to enable research into the dynamics of cell-cell interactions in human synucleinopathies and serve as a testing platform for target identification and validation of novel therapeutics.
Fri Oct 08 00:00:00 EDT 2021
A Novel Vasoactive Peptide "PG1" from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure.
Antioxidants (Basel, Switzerland)
Carrizzo A,Basilicata MG,Pepe G,Sørensen KK,Ciccarelli M,Sarno VD,Damato A,Venturini E,Borrelli A,Musella S,Abate M,Pietro PD,Ostacolo C,Campiglia P,Vecchione C
40-2200 was used in Immunocytochemistry to combine in vitro chemical assay such as LC-MS/MS analysis of buffalo ice cream, ex vivo vascular studies evaluating endothelial and smooth muscle responses using pressure myograph, and translational assay testing in vivo the vascular actions of PG1 administration in murine models.
Fri Mar 12 00:00:00 EST 2021
Multi-level inhibition of coronavirus replication by chemical ER stress.
Nature communications
Shaban MS,Müller C,Mayr-Buro C,Weiser H,Meier-Soelch J,Albert BV,Weber A,Linne U,Hain T,Babayev I,Karl N,Hofmann N,Becker S,Herold S,Schmitz ML,Ziebuhr J,Kracht M
40-2200 was used in Western Blot to provide evidence that pharmacological reprogramming of ER stress pathways can be exploited to suppress CoV replication.
Mon Sep 20 00:00:00 EDT 2021
Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial.
International journal of molecular sciences
Lin KL,Lu JH,Chueh KS,Juan TJ,Wu BN,Chuang SM,Lee YC,Shen MC,Long CY,Juan YS
40-2200 was used in Western Blot to conclude that LiESWT attenuated inflammatory responses, increased angiogenesis, and promoted proliferation and differentiation, thereby improved OAB symptoms, thereafter promoting social activity and the quality of life of postmenopausal participants.
Fri Aug 27 00:00:00 EDT 2021
Attenuated CSF-1R signalling drives cerebrovascular pathology.
EMBO molecular medicine
Delaney C,Farrell M,Doherty CP,Brennan K,O'Keeffe E,Greene C,Byrne K,Kelly E,Birmingham N,Hickey P,Cronin S,Savvides SN,Doyle SL,Campbell M
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to suggest a targeted approach to restoring BBB integrity while also restoring CSF-1R signalling in macrophages could drive a therapeutic response in ALSP patients and other AD-like dementia.
Fri Feb 05 00:00:00 EST 2021
Verapamil as an Adjunct Therapy to Reduce tPA Toxicity in Hyperglycemic Stroke: Implication of TXNIP/NLRP3 Inflammasome.
Molecular neurobiology
Ismael S,Nasoohi S,Yoo A,Mirzahosseini G,Ahmed HA,Ishrat T
40-2200 was used in Western Blotting to investigate whether verapamil, an established TXNIP inhibitor, may provide protection against hyperglycemic stroke and tPA-induced blood-brain barrier (BBB) disruption.
Sun Aug 01 00:00:00 EDT 2021
Epithelial Regeneration in Human Corneas Preserved in an Active Storage Machine.
Translational vision science & technology
Guindolet D,Crouzet E,He Z,Herbepin P,Perrache C,Garcin T,Gauthier AS,Forest F,Peoc'h M,Gain P,Gabison E,Thuret G
40-2200 was used in Immunohistochemistry-immunofluorescence to characterize the corneal epithelium (CE) and limbal epithelium (LE) of human corneas stored in an innovative active storage machine (ASM) after a period of organ culture (OC).
Fri Feb 05 00:00:00 EST 2021
ID4 Is Required for Normal Ependymal Cell Development.
Frontiers in neuroscience
Rocamonde B,Herranz-Pérez V,Garcia-Verdugo JM,Huillard E
40-2200 was used in Immunohistochemistry-immunofluorescence to provide evidence that the transcriptional regulator ID4 is involved in ependymal cell development and maturation.
Thu May 13 00:00:00 EDT 2021
Longitudinal [18F]FDG and [13N]NH3 PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model.
NeuroImage. Clinical
Zhang L,López-Picón FR,Jia Y,Chen Y,Li J,Han C,Zhuang X,Xia H
40-2200 was used in Immunohistochemistry to further understand the neurological changes induced by spinal cord injury (SCI) in its acute and subacute stages.
Fri Sep 10 00:00:00 EDT 2021
Apico-basal cell compression regulates Lamin A/C levels in epithelial tissues.
Nature communications
Iyer KV,Taubenberger A,Zeidan SA,Dye NA,Eaton S,Jülicher F
40-2200 was used in Immunocytochemistry to reveal a mechanism of Lamin A/C regulation which could provide key insights for understanding nuclear mechanotransduction in epithelial tissues.
Thu Mar 25 00:00:00 EDT 2021
Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.
Pharmaceuticals (Basel, Switzerland)
Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T
40-2200 was used in Western Blot to test the hypothesis that endothelial-specific thioredoxin-interacting protein knock-out mice will be more resistant to the neurovascular damage associated with hyperglycemia in embolic stroke.
Mon Sep 27 00:00:00 EDT 2021
APOE4 accelerates advanced-stage vascular and neurodegenerative disorder in old Alzheimer's mice via cyclophilin A independently of amyloid-β.
Nature aging
Montagne A,Nikolakopoulou AM,Huuskonen MT,Sagare AP,Lawson EJ,Lazic D,Rege SV,Grond A,Zuniga E,Barnes SR,Prince J,Sagare M,Hsu CJ,LaDu MJ,Jacobs RE,Zlokovic BV
40-2200 was used in Immunohistochemistry to show that, compared to APOE3, APOE4 accelerates blood-brain barrier (BBB) breakdown, loss of cerebral blood flow, neuronal loss and behavioral deficits independently of amyloid-β.
Tue Jun 01 00:00:00 EDT 2021
Investigation of Neurodevelopmental Deficits of 22 q11.2 Deletion Syndrome with a Patient-iPSC-Derived Blood-Brain Barrier Model.
Cells
Li Y,Xia Y,Zhu H,Luu E,Huang G,Sun Y,Sun K,Markx S,Leong KW,Xu B,Fu BM
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to suggest that neurovascular coupling can be targeted in developing novel therapeutical strategies for the treatment of 22q11.2 deletion syndrome.
Tue Sep 28 00:00:00 EDT 2021
BBS Proteins Affect Ciliogenesis and Are Essential for Hedgehog Signaling, but Not for Formation of iPSC-Derived RPE-65 Expressing RPE-Like Cells.
International journal of molecular sciences
Hey CAB,Larsen LJ,Tümer Z,Brøndum-Nielsen K,Grønskov K,Hjortshøj TD,Møller LB
40-2200 was used in Immunocytochemistry to investigate the involvement of BBS1, BBS5 and BBS10 in ciliogenesis and hedgehog signaling in Bardet-Biedl syndrome (BBS)-defective patient fibroblasts as well as in RPE-hTERT cells following siRNA-mediated knockdown of the BBS genes.
Fri Jan 29 00:00:00 EST 2021
The anesthetic sevoflurane induces tau trafficking from neurons to microglia.
Communications biology
Dong Y,Liang F,Huang L,Fang F,Yang G,Tanzi RE,Zhang Y,Quan Q,Xie Z
40-2200 was used in Immunohistochemistry to assess mice and neurons treated with anesthetics sevoflurane and desflurane, and apply nanobeam-sensor technology, an ultrasensitive method, to measure tau/p-tau amounts.
Wed May 12 00:00:00 EDT 2021
Real-Time Monitoring the Effect of Cytopathic Hypoxia on Retinal Pigment Epithelial Barrier Functionality Using Electric Cell-Substrate Impedance Sensing (ECIS) Biosensor Technology.
International journal of molecular sciences
Guerra MH,Yumnamcha T,Ebrahim AS,Berger EA,Singh LP,Ibrahim AS
40-2200 was used in Western Blot, Immunocytochemistry to demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells' viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters.
Tue Apr 27 00:00:00 EDT 2021
Real-Time Monitoring the Effect of Cytopathic Hypoxia on Retinal Pigment Epithelial Barrier Functionality Using Electric Cell-Substrate Impedance Sensing (ECIS) Biosensor Technology.
International journal of molecular sciences
Guerra MH,Yumnamcha T,Ebrahim AS,Berger EA,Singh LP,Ibrahim AS
40-2200 was used in Western Blot, Immunocytochemistry to demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells' viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters.
Tue Apr 27 00:00:00 EDT 2021
B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model.
International journal of molecular sciences
Chen CH,Liao CH,Chen KC,Wang KL,Tseng XW,Tsai WK,Chiang HS,Wu YN
40-2200 was used in Immunohistochemistry to suggest that the B6 mouse strain is more suitable for IC models.
Mon Nov 08 00:00:00 EST 2021
Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries.
eLife
Olivera GC,Ross EC,Peuckert C,Barragan A
40-2200 was used in Immunohistochemistry-immunofluorescence to reveal that the initial passage of T. gondii to the central nervous system occurs principally across cortical capillaries and that the integrity of the microvascular BBB restricts parasite transit, which conversely is exacerbated by the inflammatory response.
Wed Dec 08 00:00:00 EST 2021
Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2.
Respiratory research
Gopallawa I,Kuek LE,Adappa ND,Palmer JN,Lee RJ
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to demonstrate SC79 activation of Akt induces potentially anti-pathogenic NO production, antioxidant gene transcription, reduces IL-8 transcription, and may protect against oxidative barrier dysfunction in a wide range of airway epithelial cells.
Tue Oct 19 00:00:00 EDT 2021
Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages.
Journal of inflammation research
Wang R,Song W,Xie C,Zhong W,Xu H,Zhou Q,Deng Y,Hong Y,Li X,Fang M
40-2200 was used in Western Blot to detect zonula occludens-1 (ZO-1), occludin and claudin-5 expression in PMVECs, as well as TNF-α, TGF-β, iNOS, CD86 and CD206 expression in lungs.
Sat Apr 23 00:00:00 EDT 2022
Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages.
Journal of inflammation research
Wang R,Song W,Xie C,Zhong W,Xu H,Zhou Q,Deng Y,Hong Y,Li X,Fang M
40-2200 was used in Western Blot to detect zonula occludens-1 (ZO-1), occludin and claudin-5 expression in PMVECs, as well as TNF-α, TGF-β, iNOS, CD86 and CD206 expression in lungs.
Sat Apr 23 00:00:00 EDT 2022
Comparative assessment of in vitro BBB tight junction integrity following exposure to cigarette smoke and e-cigarette vapor: a quantitative evaluation of the protective effects of metformin using small-molecular-weight paracellular markers.
Fluids and barriers of the CNS
Kadry H,Noorani B,Bickel U,Abbruscato TJ,Cucullo L
40-2200 was used in Western Blot to quantitatively assess the damaging effects of tobacco smoke and electronic cigarettes and the protective effects of metformin on blood-brain barrier integrity.
Tue Jun 22 00:00:00 EDT 2021
Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.
Stem cell research
You J,Cheng Y,Yang XJ,Chen L
40-2200 was used in Flow Cytometry to generate an LRP2-KO human embryonic stem (ES) cell line using CRISPR/Cas9 gene editing and differentiate the mutant ES cells into RPE cells.
Sat May 01 00:00:00 EDT 2021
Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.
Stem cell research
You J,Cheng Y,Yang XJ,Chen L
40-2200 was used in Flow Cytometry to generate an LRP2-KO human embryonic stem (ES) cell line using CRISPR/Cas9 gene editing and differentiate the mutant ES cells into RPE cells.
Sat May 01 00:00:00 EDT 2021
D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degeneration.
Signal transduction and targeted therapy
Wang B,Wang L,Gu S,Yu Y,Huang H,Mo K,Xu H,Zeng F,Xiao Y,Peng L,Liu C,Cao N,Liu Y,Yuan J,Ouyang H
40-2200 was used in Immunocytochemistry to describe a high-content screening procedure with a library of 814 protective compounds and found that D609 strongly protected RPE cells from sodium iodate (SI)-induced oxidative cell death and prolonged their healthy survival.
Wed Mar 04 00:00:00 EST 2020
High-Throughput Screen Identifies Host and Microbiota Regulators of Intestinal Barrier Function.
Gastroenterology
Grosheva I,Zheng D,Levy M,Polansky O,Lichtenstein A,Golani O,Dori-Bachash M,Moresi C,Shapiro H,Del Mare-Roumani S,Valdes-Mas R,He Y,Karbi H,Chen M,Harmelin A,Straussman R,Yissachar N,Elinav E,Geiger B
40-2200 was used in Immunohistochemistry to investigate and identify molecules that disrupt and support the intestinal epithelial barrier.
Sun Nov 01 00:00:00 EDT 2020
Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.
Nature immunology
Wandel MP,Kim BH,Park ES,Boyle KB,Nayak K,Lagrange B,Herod A,Henry T,Zilbauer M,Rohde J,MacMicking JD,Randow F
40-2200 was used in Immunocytochemistry-immunoflourescence to investigate caspase-4 activation by interferon-γ-stimulated cells guanylate-binding proteins on the surface of gram-negative bacteria.
Sat Aug 01 00:00:00 EDT 2020
Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis.
The Journal of experimental medicine
Liu XX,Yang L,Shao LX,He Y,Wu G,Bao YH,Lu NN,Gong DM,Lu YP,Cui TT,Sun NH,Chen DY,Shi WX,Fukunaga K,Chen HS,Chen Z,Han F,Lu YM
40-2200 was used in Immunocytochemistry to show the development of spontaneous epilepsy can be promoted by XCL1/CXCR2-mediated reactive astrogliosis as stimulated by Cdk5 deficit.
Mon Jan 06 00:00:00 EST 2020
TIMP1 preserves the blood-brain barrier through interacting with CD63/integrin 1 complex and regulating downstream FAK/RhoA signaling.
Acta pharmaceutica Sinica. B
Tang J,Kang Y,Huang L,Wu L,Peng Y
40-2200 was used in Western Blotting to explore the neuroprotective role of TIMP1 in mice with experimental brain injury.
Mon Jun 01 00:00:00 EDT 2020
TIMP1 preserves the blood-brain barrier through interacting with CD63/integrin 1 complex and regulating downstream FAK/RhoA signaling.
Acta pharmaceutica Sinica. B
Tang J,Kang Y,Huang L,Wu L,Peng Y
40-2200 was used in Western Blotting to explore the neuroprotective role of TIMP1 in mice with experimental brain injury.
Mon Jun 01 00:00:00 EDT 2020
Syncytia formation by SARS-CoV-2-infected cells.
The EMBO journal
Buchrieser J,Dufloo J,Hubert M,Monel B,Planas D,Rajah MM,Planchais C,Porrot F,Guivel-Benhassine F,Van der Werf S,Casartelli N,Mouquet H,Bruel T,Schwartz O
40-2200 was used in Immunocytochemistry-immunoflourescence to show that SARS-CoV-2 pathological effects are modulated by cellular proteins that either inhibit or facilitate syncytia formation.
Tue Dec 01 00:00:00 EST 2020
Hedgehog Signaling Regulates Epithelial Morphogenesis to Position the Ventral Embryonic Midline.
Developmental cell
Arraf AA,Yelin R,Reshef I,Jadon J,Abboud M,Zaher M,Schneider J,Vladimirov FK,Schultheiss TM
40-2200 was used in Immunohistochemistry to investigate if there is a role for Hedgehog signaling in regulating epithelial morphology and provide an example of how transcellular signaling can modulate specific cellular processes to shape tissue morphogenesis.
Mon Jun 08 00:00:00 EDT 2020
YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling.
Development (Cambridge, England)
Cha B,Ho YC,Geng X,Mahamud MR,Chen L,Kim Y,Choi D,Kim TH,Randolph GJ,Cao X,Chen H,Srinivasan RS
40-2200 was used in Immunohistochemistry, Immunocytochemistry to report that VEGF-C signaling is necessary for valve morphogenesis.
Sun Dec 13 00:00:00 EST 2020
YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling.
Development (Cambridge, England)
Cha B,Ho YC,Geng X,Mahamud MR,Chen L,Kim Y,Choi D,Kim TH,Randolph GJ,Cao X,Chen H,Srinivasan RS
40-2200 was used in Immunohistochemistry, Immunocytochemistry to report that VEGF-C signaling is necessary for valve morphogenesis.
Sun Dec 13 00:00:00 EST 2020
Mesenchymal-epithelial transition regulates initiation of pluripotency exit before gastrulation.
Development (Cambridge, England)
Hamidi S,Nakaya Y,Nagai H,Alev C,Kasukawa T,Chhabra S,Lee R,Niwa H,Warmflash A,Shibata T,Sheng G
40-2200 was used in Immunohistochemistry (PFA fixed) to provide evidence that integrin-mediated cell-matrix interaction is a key player in pluripotency exit regulation.
Mon Feb 03 00:00:00 EST 2020
Actin-Binding Protein Cortactin Promotes Pathogenesis of Experimental Autoimmune Encephalomyelitis by Supporting Leukocyte Infiltration into the Central Nervous System.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Samus M,Li YT,Sorokin L,Rottner K,Vestweber D
40-2200 was used in Immunohistochemistry to study the role of cortactin in experimental autoimmune encephalomyelitis through the use of cortactin gene-inactivated male and female mice.
Wed Feb 12 00:00:00 EST 2020
EGF and a GSK3 Inhibitor Deplete Junctional E-cadherin and Stimulate Proliferation in the Mature Mammalian Ear.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Kozlowski MM,Rudolf MA,Corwin JT
40-2200 was used in Immunohistochemistry to report the discovery of a pharmacological treatment that thins F-actin bands, depletes E-cadherin, and stimulates proliferation in long-quiescent supporting cells within a balance epithelium from adult mice.
Wed Mar 25 00:00:00 EDT 2020
Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.
Frontiers in bioengineering and biotechnology
Altay G,Tosi S,García-Díaz M,Martínez E
40-2200 was used in Immunocytochemistry-immunoflourescence to study the morphology of cells in 3D engineered intestinal tissues.
Mon Sep 28 00:00:00 EDT 2020
Treatment with interleukin-33 is non-toxic and protects retinal pigment epithelium in an ageing model of outer retinal degeneration.
Journal of cellular and molecular medicine
Clare AJ,Copland DA,Nicholson LB,Liu J,Neal CR,Moss S,Dick AD,Theodoropoulou S
40-2200 was used in Immunohistochemistry to investigate IL-33 delivery in aged, high-fat diet (HFD) fed mice on a wildtype and complement factor H heterozygous knockout background.
Sun Nov 01 00:00:00 EDT 2020
Nf2 fine-tunes proliferation and tissue alignment during closure of the optic fissure in the embryonic mouse eye.
Human molecular genetics
Sun WR,Ramirez S,Spiller KE,Zhao Y,Fuhrmann S
40-2200 was used in Immunohistochemistry (Frozen) to indicate that limiting proliferation particularly in the RPE layer is a critical mechanism during optic fissure closure.
Fri Dec 18 00:00:00 EST 2020
Modulation of Tmem135 Leads to Retinal Pigmented Epithelium Pathologies in Mice.
Investigative ophthalmology & visual science
Landowski M,Grindel S,Shahi PK,Johnson A,Western D,Race A,Shi F,Benson J,Gao M,Santoirre E,Lee WH,Ikeda S,Pattnaik BR,Ikeda A
40-2200 was used in Immunohistochemistry to identify transmembrane protein 135 (Tmem135) as a gene important for regulating retinal aging and mitochondrial dynamics in mice.
Thu Oct 01 00:00:00 EDT 2020
An organoid model to assay the role of CFTR in the human epididymis epithelium.
Cell and tissue research
Leir SH,Yin S,Kerschner JL,Xia S,Ahmadi S,Bear C,Harris A
40-2200 was used in Immunocytochemistry to facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Sat Aug 01 00:00:00 EDT 2020
Human Fallopian Tube Epithelial Cell Culture Model To Study Host Responses to Chlamydia trachomatis Infection.
Infection and immunity
McQueen BE,Kiatthanapaiboon A,Fulcher ML,Lam M,Patton K,Powell E,Kollipara A,Madden V,Suchland RJ,Wyrick P,O'Connell CM,Reidel B,Kesimer M,Randell SH,Darville T,Nagarajan UM
40-2200 was used in Immunocytochemistry to develop a primary human fallopian tube epithelial cell model for studying chlamydia trachomatis infection.
Wed Aug 19 00:00:00 EDT 2020
Soy Protein Nanofiber Scaffolds for Uniform Maturation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium.
Tissue engineering. Part C, Methods
Phelan MA,Kruczek K,Wilson JH,Brooks MJ,Drinnan CT,Regent F,Gerstenhaber JA,Swaroop A,Lelkes PI,Li T
40-2200 was used in Immunohistochemistry to describe the use of electroprocessed nanofibrous soy protein scaffolds to generate polarized sheets of human iPSC-derived iRPE sheets.
Sat Aug 01 00:00:00 EDT 2020
Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture.
Experimental and therapeutic medicine
Li Y,Guo R,Zhang M,Chen P,Li J,Sun Y
40-2200 was used in Western Blotting to investigate the role of emodin on intestinal epithelial tight junction barrier integrity.
Mon Jun 01 00:00:00 EDT 2020
Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture.
Experimental and therapeutic medicine
Li Y,Guo R,Zhang M,Chen P,Li J,Sun Y
40-2200 was used in Western Blotting to investigate the role of emodin on intestinal epithelial tight junction barrier integrity.
Mon Jun 01 00:00:00 EDT 2020
The cerebrovascular and neurological impact of chronic smoking on post-traumatic brain injury outcome and recovery: an in vivo study.
Journal of neuroinflammation
Sivandzade F,Alqahtani F,Sifat A,Cucullo L
40-2200 was used in Western Blotting to investigate the mechanisms underlying the exacerbation of TBI injury by TS using a weight drop model.
Mon Apr 27 00:00:00 EDT 2020
Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung's disease.
Scientific reports
Dariel A,Grynberg L,Auger M,Lefèvre C,Durand T,Aubert P,Le Berre-Scoul C,Venara A,Suply E,Leclair MD,de Vries P,Levard G,Parmentier B,Podevin G,Schmitt F,Couvrat V,Irtan S,Hervieux E,Villemagne T,Lardy H,Capito C,Muller C,Sarnacki S,Mosnier JF,Galmiche L,Derkinderen P,Boudin H,Brochard C,Neunlist M
40-2200 was used in Western Blotting to show that modulating neuronal phenotype and enhancing IEB permeability may treat or prevent postoperative complications in HSCR.
Thu Dec 10 00:00:00 EST 2020
HIV Tat-mediated induction of autophagy regulates the disruption of ZO-1 in brain endothelial cells.
Tissue barriers
Liao K,Niu F,Hu G,Guo ML,Sil S,Buch S
40-2200 was used in Immunocytochemistry-immunoflourescence to identify autophagy as an important mechanism underlying Tat-mediated disruption of the BBB.
Thu Apr 02 00:00:00 EDT 2020
Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration.
Cell reports
Mulfaul K,Ozaki E,Fernando N,Brennan K,Chirco KR,Connolly E,Greene C,Maminishkis A,Salomon RG,Linetsky M,Natoli R,Mullins RF,Campbell M,Doyle SL
40-2200 was used in Immunocytochemistry-Immunofluorescence to implicate TLR2 as a mediator of retinal degeneration in response to oxidative stress.
Tue Feb 18 00:00:00 EST 2020
Targeting QKI-7 in vivo restores endothelial cell function in diabetes.
Nature communications
Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A
40-2200 was used in Immunocytochemistry-immunoflourescence to show that Quaking-7 (QKI-7) is upregulated in iPS cell-derived endothelial cells(ECs) when exposed to hyperglycemia, and in human iPS-ECs from diabetic patients.
Thu Jul 30 00:00:00 EDT 2020
Interaction of Staphylococcus aureus and Host Cells upon Infection of Bronchial Epithelium during Different Stages of Regeneration.
ACS infectious diseases
Palma Medina LM,Becker AK,Michalik S,Surmann K,Hildebrandt P,Gesell Salazar M,Mekonnen SA,Kaderali L,Völker U,van Dijl JM
40-2200 was used in Immunocytochemistry-immunoflourescence to provide a deeper insight into how S. aureus can take advantage of a breached epithelial barrier and show that infected epithelial cells have limited ability to respond adequately to staphylococcal insults.
Fri Aug 14 00:00:00 EDT 2020
S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling.
JCI insight
Geng X,Yanagida K,Akwii RG,Choi D,Chen L,Ho Y,Cha B,Mahamud MR,Berman de Ruiz K,Ichise H,Chen H,Wythe JD,Mikelis CM,Hla T,Srinivasan RS
40-2200 was used in Immunohistochemistry to show that the canonical tip cell marker Delta-like 4 (DLL4) promotes sprouting lymphangiogenesis by enhancing VEGF-C/VEGF receptor 3 (VEGFR3) signaling.
Thu Jul 23 00:00:00 EDT 2020
Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance.
Scientific reports
Roudnicky F,Kim BK,Lan Y,Schmucki R,Küppers V,Christensen K,Graf M,Patsch C,Burcin M,Meyer CA,Westenskow PD,Cowan CA
40-2200 was used in Immunocytochemistry to predict TFs that induce high-resistance barrier properties of ECs as in the BBB.
Tue Mar 03 00:00:00 EST 2020
Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.
bioRxiv : the preprint server for biology
Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M
40-2200 was used in Immunohistochemistry-immunofluorescence to establish an airway epithelium model to study SARS-CoV-2 infection in healthy and COPD lung cells.
Thu Nov 12 00:00:00 EST 2020
Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.
bioRxiv : the preprint server for biology
Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M
40-2200 was used in Immunohistochemistry-immunofluorescence to establish an airway epithelium model to study SARS-CoV-2 infection in healthy and COPD lung cells.
Thu Nov 12 00:00:00 EST 2020
Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells.
Nature communications
Plaza Reyes A,Petrus-Reurer S,Padrell Sánchez S,Kumar P,Douagi I,Bartuma H,Aronsson M,Westman S,Lardner E,André H,Falk A,Nandrot EF,Kvanta A,Lanner F
40-2200 was used in Immunocytochemistry to identify cell surface markers for retinal pigment epithelial cells and develop a robust, direct and scalable monolayer differentiation protocol.
Mon Mar 30 00:00:00 EDT 2020
Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform.
Nature communications
Kutys ML,Polacheck WJ,Welch MK,Gagnon KA,Koorman T,Kim S,Li L,McClatchey AI,Chen CS
40-2200 was used in Immunocytochemistry-immunoflourescence to demonstrate the functionality of a model system that facilitates the dissection of 3D morphogenic behaviors and bidirectional signaling between mammary epithelium and endothelium during homeostasis and pathogenesis.
Mon Jul 06 00:00:00 EDT 2020
Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells.
Nature communications
Zhu N,Wang W,Liu Z,Liang C,Wang W,Ye F,Huang B,Zhao L,Wang H,Zhou W,Deng Y,Mao L,Su C,Qiang G,Jiang T,Zhao J,Wu G,Song J,Tan W
40-2200 was used in Immunocytochemistry-immunoflourescence to provide an insight into SARS-CoV-2 cell tropism, replication and morphogenesis in human airway epithelial cultures.
Thu Aug 06 00:00:00 EDT 2020
Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.
Aging
Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z
40-2200 was used in Immunohistochemistry-immunofluorescence to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.
Mon Jun 22 00:00:00 EDT 2020
Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.
Aging
Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z
40-2200 was used in Immunohistochemistry-immunofluorescence to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.
Mon Jun 22 00:00:00 EDT 2020
Anisakis simplex products impair intestinal epithelial barrier function and occludin and zonula occludens-1 localisation in differentiated Caco-2 cells.
PLoS neglected tropical diseases
Carballeda-Sangiao N,Sánchez-Alonso I,Navas A,Arcos SC,de Palencia PF,Careche M,González-Muñoz M
40-2200 was used in Immunocytochemistry-immunoflourescence to study the effect of A. simplex crude extract on the intestinal integrity and permeability by using Caco-2 cell monolayer.
Wed Jul 01 00:00:00 EDT 2020
Site-specific opening of the blood-brain barrier by extracellular histones.
Journal of neuroinflammation
Villalba N,Baby S,Cha BJ,Yuan SY
40-2200 was used in Immunocytochemistry-immunoflourescence to suggest that circulating histones may contribute to cerebrovascular injury or brain dysfunction by altering BBB structure and function.
Tue Sep 22 00:00:00 EDT 2020
Site-specific opening of the blood-brain barrier by extracellular histones.
Journal of neuroinflammation
Villalba N,Baby S,Cha BJ,Yuan SY
40-2200 was used in Immunocytochemistry-immunoflourescence to suggest that circulating histones may contribute to cerebrovascular injury or brain dysfunction by altering BBB structure and function.
Tue Sep 22 00:00:00 EDT 2020
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
STAR protocols
Hor P,Ichida JK,Borok Z,Ryan AL
40-2200 was used in Immunocytochemistry-immunoflourescence to develop a protocol for induced pulmonary neuroendocrine cells generation.
Fri Sep 18 00:00:00 EDT 2020
JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice.
The FEBS journal
Liu Y,Fan J,Yan Y,Dang X,Zhao R,Xu Y,Ding Z
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to exhibit that JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells.
Tue Dec 01 00:00:00 EST 2020
JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice.
The FEBS journal
Liu Y,Fan J,Yan Y,Dang X,Zhao R,Xu Y,Ding Z
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to exhibit that JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells.
Tue Dec 01 00:00:00 EST 2020
Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation.
Immunity
Noval Rivas M,Wakita D,Franklin MK,Carvalho TT,Abolhesn A,Gomez AC,Fishbein MC,Chen S,Lehman TJ,Sato K,Shibuya A,Fasano A,Kiyono H,Abe M,Tatsumoto N,Yamashita M,Crother TR,Shimada K,Arditi M
40-2200 was used in Immunohistochemistry to investigate intestinal barrier function in vasculitis and observe evidence of intestinal permeability and elevated circulating secretory immunoglobulin A (sIgA) in patients with Kawaski disease, as well as elevated sIgA and IgA deposition in vascular tissues in a mouse model of vasculitis.
Tue Sep 17 00:00:00 EDT 2019
Dengue virus-elicited tryptase induces endothelial permeability and shock.
The Journal of clinical investigation
Rathore AP,Mantri CK,Aman SA,Syenina A,Ooi J,Jagaraj CJ,Goh CC,Tissera H,Wilder-Smith A,Ng LG,Gubler DJ,St John AL
40-2200 was used in Immunocytochemistry-immunoflourescence to define an immune mechanism by which Dengue virus can induce vascular pathology and shock.
Tue Jul 02 00:00:00 EDT 2019
Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members.
Neuron
Ortiz-Álvarez G,Daclin M,Shihavuddin A,Lansade P,Fortoul A,Faucourt M,Clavreul S,Lalioti ME,Taraviras S,Hippenmeyer S,Livet J,Meunier A,Genovesio A,Spassky N
40-2200 was used in Immunohistochemistry-immunofluorescence to reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adult neural stem cells.
Wed Apr 03 00:00:00 EDT 2019
p120ctn-Mediated Organ Patterning Precedes and Determines Pancreatic Progenitor Fate.
Developmental cell
Nyeng P,Heilmann S,Löf-Öhlin ZM,Pettersson NF,Hermann FM,Reynolds AB,Semb H
40-2200 was used in Immunohistochemistry to reconciletwo apparently opposing models, demonstrating that pancreatic progenitors re-localize to establish the niche that will determine their ultimate fate.
Mon Apr 08 00:00:00 EDT 2019
Human complement factor H Y402H polymorphism causes an age-related macular degeneration phenotype and lipoprotein dysregulation in mice.
Proceedings of the National Academy of Sciences of the United States of America
Landowski M,Kelly U,Klingeborn M,Groelle M,Ding JD,Grigsby D,Bowes Rickman C
40-2200 was used in Immunohistochemistry-immunofluorescence to determine the effect of the principal age-related macular degeneration risk-associated complement factor H variant (Y402H) on the development and progression of age-dependent AMD-like pathologies in vivo.
Tue Feb 26 00:00:00 EST 2019
Quantitative Label-Free Imaging of 3D Vascular Networks Self-Assembled in Synthetic Hydrogels.
Advanced healthcare materials
Kaushik G,Gil DA,Torr E,Berge ES,Soref C,Uhl P,Fontana G,Antosiewicz-Bourget J,Edington C,Schwartz MP,Griffith LG,Thomson JA,Skala MC,Daly WT,Murphy WL
40-2200 was used in Immunocytochemistry to demonstrate that aMPM allows label-free monitoring of vascular network morphology to streamline optimisation of growth conditions and provide quality control of engineered tissues.
Tue Jan 01 00:00:00 EST 2019
Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.
Gut microbes
Jang YJ,Kim WK,Han DH,Lee K,Ko G
40-2200 was used in Western Blotting to evaluate immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.
Mon Feb 10 00:00:00 EST 2020
Perlecan regulates pericyte dynamics in the maintenance and repair of the blood-brain barrier.
The Journal of cell biology
Nakamura K,Ikeuchi T,Nara K,Rhodes CS,Zhang P,Chiba Y,Kazuno S,Miura Y,Ago T,Arikawa-Hirasawa E,Mukouyama YS,Yamada Y
40-2200 was used in Western Blotting to show that perlecan aids in blood-brain barrier (BBB) maintenance and repair through pericyte interactions.
Mon Oct 07 00:00:00 EDT 2019
Canalicular domain structure and function in matrix-free hepatic spheroids.
Biomaterials science
Sharma VR,Shrivastava A,Gallet B,Karepina E,Charbonnier P,Chevallet M,Jouneau PH,Deniaud A
40-2200 was used in Immunocytochemistry to show that this study is the first comprehensive and in-depth functional and structural study of bile canaliculi in a physiological-relevant context.
Tue Dec 17 00:00:00 EST 2019
Metformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage.
Oxidative medicine and cellular longevity
Ye J,Luo D,Xu X,Sun M,Su X,Tian Z,Zhang M,Yu C,Guan Q
40-2200 was used in Immunohistochemistry-immunofluorescence to explore the effects of metformin and characterising its underlying mechanism in the fertility of obese males.
Fri Mar 20 00:00:00 EDT 2020
Metformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage.
Oxidative medicine and cellular longevity
Ye J,Luo D,Xu X,Sun M,Su X,Tian Z,Zhang M,Yu C,Guan Q
40-2200 was used in Immunohistochemistry-immunofluorescence to explore the effects of metformin and characterising its underlying mechanism in the fertility of obese males.
Fri Mar 20 00:00:00 EDT 2020
DL-3-n-butylphthalide protects the blood-brain barrier against ischemia/hypoxia injury via upregulation of tight junction proteins.
Chinese medical journal
Ye ZY,Xing HY,Wang B,Liu M,Lv PY
40-2200 was used in Immunohistochemistry to investigate the potential effect of DL-3-n-butylphthalide on the tight junction proteins claudin-5, zonula occludens-1, and occludin during brain ischemia.
Wed Jun 05 00:00:00 EDT 2019
Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure.
Journal of cell science
Butler MB,Short NE,Maniou E,Alexandre P,Greene NDE,Copp AJ,Galea GL
40-2200 was used in Immunohistochemistry to show arresting cell cycle progression with hydroxyurea prevents posterior neuropore widening following Rock inhibition.
Mon Jul 01 00:00:00 EDT 2019
Enhanced gastrointestinal passive paracellular permeability contributes to the obesity-associated hyperoxaluria.
American journal of physiology. Gastrointestinal and liver physiology
Bashir M,Meddings J,Alshaikh A,Jung D,Le K,Amin R,Ratakonda S,Sharma S,Granja I,Satti M,Asplin J,Hassan H
40-2200 was used in Western Blotting to test the hypothesis that the obesity-associated enhanced intestinal paracellular permeability contributes to obesity-associated hyperoxaluria by increasing passive paracellular intestinal oxalate absorption.
Tue Jan 01 00:00:00 EST 2019
Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation.
Journal of materials science. Materials in medicine
Kennedy S,Lace R,Carserides C,Gallagher AG,Wellings DA,Williams RL,Levis HJ
40-2200 was used in Immunocytochemistry to investigate the application of pεK hydrogels as a potential synthetic substrate for the expansion and growth of human corneal endothelial cells.
Wed Sep 04 00:00:00 EDT 2019
Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation.
Journal of materials science. Materials in medicine
Kennedy S,Lace R,Carserides C,Gallagher AG,Wellings DA,Williams RL,Levis HJ
40-2200 was used in Immunocytochemistry to investigate the application of pεK hydrogels as a potential synthetic substrate for the expansion and growth of human corneal endothelial cells.
Wed Sep 04 00:00:00 EDT 2019
Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide.
Experimental eye research
Hazim RA,Volland S,Yen A,Burgess BL,Williams DS
40-2200 was used in Immunocytochemistry-immunoflourescence to identify the presence of nicotinamide, a member of the vitamin B family, as an essential factor in promoting the observed differentiation, indicating the importance of metabolism in RPE cell differentiation.
Fri Feb 01 00:00:00 EST 2019
GPR55-mediated effects on brain microvascular endothelial cells and the blood-brain barrier.
Neuroscience
Leo LM,Familusi B,Hoang M,Smith R,Lindenau K,Sporici KT,Brailoiu E,Abood ME,Brailoiu GC
40-2200 was used in Immunocytochemistry to examine the effect of GPR55 activation on rat brain microvascular endothelial cells, an essential component of the blood-brain barrier. GPR55 was detected by western blot and immunocytochemistry.
Wed Aug 21 00:00:00 EDT 2019
Nanoparticle induced barrier function assessment at liquid-liquid and air-liquid interface in novel human lung epithelia cell lines.
Toxicology research
Leibrock L,Wagener S,Singh AV,Laux P,Luch A
40-2200 was used in Immunocytochemistry-immunoflourescence to investigate the barrier function of the recently developed human lung cell lines CI-hAELVi and CI-huAEC.
Fri Nov 01 00:00:00 EDT 2019
Generation of an integration-free induced pluripotent stem cell line (FDEENTi003-A) from a patient with pathological myopia.
Stem cell research
Bai X,Yang XJ,Chen L
40-2200 was used in Immunocytochemistry to reprogramme peripheral blood mononuclear cells from pathological myopia patients into induced pluripotent stem cells.
Thu Aug 01 00:00:00 EDT 2019
Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents.
Nature communications
Zou T,Gao L,Zeng Y,Li Q,Li Y,Chen S,Hu X,Chen X,Fu C,Xu H,Yin ZQ
40-2200 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to characterize the pattern of integration and materials transfer following transplantation, which likely contribute to the rescued photoreceptors.
Thu Mar 14 00:00:00 EDT 2019
The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.
Theranostics
Yu F,Zhang T,Zhou C,Xu H,Guo L,Chen M,Wu B
40-2200 was used in Western Blotting to study how Bmal1 coordinates diurnal MRP2 expression.
Thu Jun 18 00:00:00 EDT 2020
Hepatic progenitor cell activation is induced by the depletion of the gut microbiome in mice.
MicrobiologyOpen
Wang F,Sun NN,Li LL,Zhu WW,Xiu J,Shen Y,Xu Q
40-2200 was used in Immunohistochemistry to suggest that liver function is affected by the composition of the gut microbiome.
Tue Oct 01 00:00:00 EDT 2019
Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line.
Toxins
Seyed Toutounchi N,Hogenkamp A,Varasteh S,Van't Land B,Garssen J,Kraneveld AD,Folkerts G,Braber S
40-2200 was used in Western Blot, Immunocytochemistry to investigate the effects of Fusarium mycotoxins on human placental epithelial cells.
Thu Nov 14 00:00:00 EST 2019
Fusarium Mycotoxins Disrupt the Barrier and Induce IL-6 Release in a Human Placental Epithelium Cell Line.
Toxins
Seyed Toutounchi N,Hogenkamp A,Varasteh S,Van't Land B,Garssen J,Kraneveld AD,Folkerts G,Braber S
40-2200 was used in Western Blot, Immunocytochemistry to investigate the effects of Fusarium mycotoxins on human placental epithelial cells.
Thu Nov 14 00:00:00 EST 2019
Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies.
Scientific reports
Kang HM,Lim JH,Noh KH,Park D,Cho HS,Susztak K,Jung CR
40-2200 was used in Immunocytochemistry-immunoflourescence to develop a new differentiation method to generate kidney spheroids that structurally recapitulate important features of the kidney effectively and reproducibly using mixed immortalized renal cells, and show their application for renal toxicity studies.
Tue Nov 26 00:00:00 EST 2019
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
Cell stem cell
Artegiani B,van Voorthuijsen L,Lindeboom RGH,Seinstra D,Heo I,Tapia P,López-Iglesias C,Postrach D,Dayton T,Oka R,Hu H,van Boxtel R,van Es JH,Offerhaus J,Peters PJ,van Rheenen J,Vermeulen M,Clevers H
40-2200 was used in Immunohistochemistry-immunofluorescence to find that BAP1 controls the expression of junctional and cytoskeleton components by regulating chromatin accessibility.
Thu Jun 06 00:00:00 EDT 2019
Innovative corneal active storage machine for long-term eye banking.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Garcin T,Gauthier AS,Crouzet E,He Z,Herbepin P,Perrache C,Acquart S,Cognasse F,Forest F,Thuret G,Gain P
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to show that, with an active storage machine, also called a bioreactor, which restores 2 fundamental physiological parameters, intraocular pressure and medium renewal, endothelial cell survival is improved by 23% compared with organ culture after 4 weeks' storage.
Sat Jun 01 00:00:00 EDT 2019
Brain Endothelial Cells Maintain Lactate Homeostasis and Control Adult Hippocampal Neurogenesis.
Cell stem cell
Wang J,Cui Y,Yu Z,Wang W,Cheng X,Ji W,Guo S,Zhou Q,Wu N,Chen Y,Chen Y,Song X,Jiang H,Wang Y,Lan Y,Zhou B,Mao L,Li J,Yang H,Guo W,Yang X
40-2200 was used in Western Blot to delineate how the brain endothelium maintains lactate homeostasis and contributes to adult hippocampal neurogenesis and cognitive functions.
Thu Dec 05 00:00:00 EST 2019
Japanese encephalitis virus neuropenetrance is driven by mast cell chymase.
Nature communications
Hsieh JT,Rathore APS,Soundarajan G,St John AL
40-2200 was used in Western Blotting to study the mechanisms of Japanese encephalitis at the blood brain barrier.
Mon Feb 11 00:00:00 EST 2019
The role of mutations associated with familial neurodegenerative disorders on blood-brain barrier function in an iPSC model.
Fluids and barriers of the CNS
Katt ME,Mayo LN,Ellis SE,Mahairaki V,Rothstein JD,Cheng L,Searson PC
40-2200 was used in Western Blotting to suggest that the accumulation of defects in brain microvascular endothelial cells may ultimately lead to impairment of the BBB and that accumulated defects that result in positive feedback may lead to unrelated central nervous system diseases.
Mon Jul 15 00:00:00 EDT 2019
Foxc2 is essential for podocyte function.
Physiological reports
Nilsson D,Heglind M,Arani Z,Enerbäck S
40-2200 was used in Immunohistochemistry-immunofluorescence to highlight the importance of the Foxc2 gene for the function of podocyte.
Wed May 01 00:00:00 EDT 2019
BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration.
Nature communications
Liu M,Sun T,Li N,Peng J,Fu D,Li W,Li L,Gao WQ
40-2200 was used in Western Blot to report that BRG1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for the homeostatic maintenance of intestinal epithelial cells (IECs) to prevent the inflammation and tumorigenesis.
Thu Oct 10 00:00:00 EDT 2019
BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration.
Nature communications
Liu M,Sun T,Li N,Peng J,Fu D,Li W,Li L,Gao WQ
40-2200 was used in Western Blot to report that BRG1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for the homeostatic maintenance of intestinal epithelial cells (IECs) to prevent the inflammation and tumorigenesis.
Thu Oct 10 00:00:00 EDT 2019
Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration.
eLife
Storti F,Klee K,Todorova V,Steiner R,Othman A,van der Velde-Visser S,Samardzija M,Meneau I,Barben M,Karademir D,Pauzuolyte V,Boye SL,Blaser F,Ullmer C,Dunaief JL,Hornemann T,Rohrer L,den Hollander A,von Eckardstein A,Fingerle J,Maugeais C,Grimm C
40-2200 was used in Immunohistochemistry-immunofluorescence to highlight the essential homeostatic role for lipid efflux in the RPE and suggest a pathogenic contribution of reduced ABCA1 function to AMD.
Wed Mar 13 00:00:00 EDT 2019
Dysregulated claudin-5 cycling in the inner retina causes retinal pigment epithelial cell atrophy.
JCI insight
Hudson N,Celkova L,Hopkins A,Greene C,Storti F,Ozaki E,Fahey E,Theodoropoulou S,Kenna PF,Humphries MM,Curtis AM,Demmons E,Browne A,Liddie S,Lawrence MS,Grimm C,Cahill MT,Humphries P,Doyle SL,Campbell M
40-2200 was used in Immunohistochemistry-immunofluorescence to implicate an inner retina-derived component in the early pathophysiological changes observed in AMD, and suggest that restoring the integrity of the inner blood-retina barrier may represent a novel therapeutic target.
Thu Aug 08 00:00:00 EDT 2019
Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers.
The Journal of cell biology
Hilfenhaus G,Nguyen DP,Freshman J,Prajapati D,Ma F,Song D,Ziyad S,Cuadrado M,Pellegrini M,Bustelo XR,Iruela-Arispe ML
40-2200 was used in Immunocytochemistry to identify cytoskeletal regulators that underlie barrier diversity across vessels.
Mon Aug 06 00:00:00 EDT 2018
Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers.
The Journal of cell biology
Hilfenhaus G,Nguyen DP,Freshman J,Prajapati D,Ma F,Song D,Ziyad S,Cuadrado M,Pellegrini M,Bustelo XR,Iruela-Arispe ML
40-2200 was used in Immunocytochemistry to identify cytoskeletal regulators that underlie barrier diversity across vessels.
Mon Aug 06 00:00:00 EDT 2018
CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro.
International journal of cancer
Luo J,Chimge NO,Zhou B,Flodby P,Castaldi A,Firth AL,Liu Y,Wang H,Yang C,Marconett CN,Crandall ED,Offringa IA,Frenkel B,Borok Z
40-2200 was used in Immunocytochemistry to indicate a tumor suppressor role for CLDN18.1 in LuAd mediated by a regulatory network that encompasses YAP/TAZ, IGF-1R and AKT signaling.
Sat Dec 15 00:00:00 EST 2018
Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions.
Development (Cambridge, England)
Liu X,Gu X,Ma W,Oxendine M,Gil HJ,Davis GE,Cleaver O,Oliver G
40-2200 was used in Immunocytochemistry-immunoflourescence to study the role of Rasip1 in lumen maintenance.
Mon Aug 20 00:00:00 EDT 2018
Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions.
Development (Cambridge, England)
Liu X,Gu X,Ma W,Oxendine M,Gil HJ,Davis GE,Cleaver O,Oliver G
40-2200 was used in Immunocytochemistry-immunoflourescence to study the role of Rasip1 in lumen maintenance.
Mon Aug 20 00:00:00 EDT 2018
Comparison of preservation and transportation protocols for preloaded Descemet membrane endothelial keratoplasty.
The British journal of ophthalmology
Romano V,Parekh M,Ruzza A,Willoughby CE,Ferrari S,Ponzin D,Kaye SB,Levis HJ
40-2200 was used in Immunocytochemistry-immunoflourescence to show HEC analysis provides further detailed information as to the status of DMEK grafts and should be used in future similar studies.
Sun Apr 01 00:00:00 EDT 2018
Characterizing well-differentiated culture of primary human nasal epithelial cells for use in wound healing assays.
Laboratory investigation; a journal of technical methods and pathology
Schagen J,Sly PD,Fantino E
40-2200 was used in Immunocytochemistry-immunoflourescence to characterise human primary nasal epithelial cells grown at the air-liquid interface and establish a model for use in wound healing assays.
Thu Nov 01 00:00:00 EDT 2018
ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner.
American journal of physiology. Cell physiology
Bilal S,Jaggi S,Janosevic D,Shah N,Teymour S,Voronina A,Watari J,Axis J,Amsler K
40-2200 was used in Western Blotting to demonstrate that H2O2 increases MDCK cell paracellular permeability through activation of ERK 1/2.
Sat Sep 01 00:00:00 EDT 2018
Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis.
American journal of physiology. Cell physiology
Alsaffar H,Martino N,Garrett JP,Adam AP
40-2200 was used in Western Blotting to show that interleukin-6 (IL-6) promotes an increase in endothelial monolayer permeability that lasts over 24 h and demonstrate that activation of Src and MEK/ERK pathways is required only for short-term increases in permeability, being dispensable after 2 h.
Tue May 01 00:00:00 EDT 2018
Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis.
American journal of physiology. Cell physiology
Alsaffar H,Martino N,Garrett JP,Adam AP
40-2200 was used in Western Blotting to show that interleukin-6 (IL-6) promotes an increase in endothelial monolayer permeability that lasts over 24 h and demonstrate that activation of Src and MEK/ERK pathways is required only for short-term increases in permeability, being dispensable after 2 h.
Tue May 01 00:00:00 EDT 2018
A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.
Molecular human reproduction
Yang R,Browne JA,Eggener SE,Leir SH,Harris A
40-2200 was used in Immunocytochemistry-immunoflourescence to suggest a novel AR transcriptional network governs differentiated functions of the human epididymis epithelium.
Sat Sep 01 00:00:00 EDT 2018
Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.
ASAIO journal (American Society for Artificial Internal Organs : 1992)
Ferrell N,Cheng J,Miao S,Roy S,Fissell WH
40-2200 was used in Immunocytochemistry-immunoflourescence to evaluate the phenotype of primary human renal tubular epithelial cells subjected to fluid shear stress by culturing in orbital shaker.
Wed Sep 04 00:00:00 EDT 2019
Photoreceptor Outer Segment Isolation from a Single Canine Retina for RPE Phagocytosis Assay.
Advances in experimental medicine and biology
Sudharsan R,Elliott MH,Dolgova N,Aguirre GD,Beltran WA
40-2200 was used in Immunocytochemistry-immunoflourescence to present an optimised protocol for photoreceptor outer segment isolation, using a single canine retina, that can be used for retinal pigment epithelium phagocytosis assays.
Mon May 06 00:00:00 EDT 2019
Cardiac specific PRMT1 ablation causes heart failure through CaMKII dysregulation.
Nature communications
Pyun JH,Kim HJ,Jeong MH,Ahn BY,Vuong TA,Lee DI,Choi S,Koo SH,Cho H,Kang JS
40-2200 was used in Immunocytochemistry-immunoflourescence to suggest that PRMT1 is a critical regulator of CaMKII to maintain cardiac function.
Fri Nov 30 00:00:00 EST 2018
Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity.
Cell reports
Lee S,Cieply B,Yang Y,Peart N,Glaser C,Chan P,Carstens RP
40-2200 was used in Immunocytochemistry-immunoflourescence to study the mechanisms by which Esrp ablation causes defects in epithelial barriers.
Tue Nov 27 00:00:00 EST 2018
Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity.
Cell reports
Lee S,Cieply B,Yang Y,Peart N,Glaser C,Chan P,Carstens RP
40-2200 was used in Immunocytochemistry-immunoflourescence to study the mechanisms by which Esrp ablation causes defects in epithelial barriers.
Tue Nov 27 00:00:00 EST 2018
A convenient protocol for establishing a human cell culture model of the outer retina.
F1000Research
Lynn SA,Keeling E,Dewing JM,Johnston DA,Page A,Cree AJ,Tumbarello DA,Newman TA,Lotery AJ,Ratnayaka JA
40-2200 was used in Immunocytochemistry-immunoflourescence to present a protocol for the rapid establishment and study of retinal pigment epithelium (RPE) cells in long-term culture, using the human ARPE-19 cell-line.
Wed Sep 04 00:00:00 EDT 2019
Collagen vitrigels with low-fibril density enhance human embryonic stem cell-derived retinal pigment epithelial cell maturation.
Journal of tissue engineering and regenerative medicine
Wang X,Maruotti J,Majumdar S,Roman J,Mao HQ,Zack DJ,Elisseeff JH
40-2200 was used in Immunocytochemistry-immunofluorescence to provide insight into the influence of collagen nanofibrillar structures on hESC-RPE maturation and presents a potential bioengineered substratum for hESC-RPE for future preclinical and clinical applications.
Thu Mar 01 00:00:00 EST 2018
Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers.
International journal of molecular sciences
Sfakis L,Kamaldinov T,Khmaladze A,Hosseini ZF,Nelson DA,Larsen M,Castracane J
40-2200 was used in Immunocytochemistry to demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro.
Thu Mar 29 00:00:00 EDT 2018
A novel dynamic multicellular co-culture system for studying individual blood-brain barrier cell types in brain diseases and cytotoxicity testing.
Scientific reports
Miranda-Azpiazu P,Panagiotou S,Jose G,Saha S
40-2200 was used in Immunocytochemistry to develop and optimise an in vitro multicellular co-culture model within the Kirkstall Quasi Vivo System.
Fri Jun 08 00:00:00 EDT 2018
Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis.
Scientific reports
Hayes CL,Dong J,Galipeau HJ,Jury J,McCarville J,Huang X,Wang XY,Naidoo A,Anbazhagan AN,Libertucci J,Sheridan C,Dudeja PK,Bowdish DME,Surette MG,Verdu EF
40-2200 was used in Immunohistochemistry-immunofluorescence to study the role of microbiota in intestinal barrier function.
Fri Sep 21 00:00:00 EDT 2018
Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands.
Journal of anatomy
Zhang XM,Huang Y,Zhang K,Qu LH,Cong X,Su JZ,Wu LL,Yu GY,Zhang Y
40-2200 was used in Immunohistochemistry-immunofluorescence to show that porcine parotid glands were pure serous glands and show the expression pattern of TJ proteins in salivary glands is similar between human and miniature pig, which may be a candidate animal for studies on salivary gland TJ function.
Wed Aug 01 00:00:00 EDT 2018
Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands.
Journal of anatomy
Zhang XM,Huang Y,Zhang K,Qu LH,Cong X,Su JZ,Wu LL,Yu GY,Zhang Y
40-2200 was used in Immunohistochemistry-immunofluorescence to show that porcine parotid glands were pure serous glands and show the expression pattern of TJ proteins in salivary glands is similar between human and miniature pig, which may be a candidate animal for studies on salivary gland TJ function.
Wed Aug 01 00:00:00 EDT 2018
Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands.
Journal of anatomy
Zhang XM,Huang Y,Zhang K,Qu LH,Cong X,Su JZ,Wu LL,Yu GY,Zhang Y
40-2200 was used in Immunohistochemistry-immunofluorescence to show that porcine parotid glands were pure serous glands and show the expression pattern of TJ proteins in salivary glands is similar between human and miniature pig, which may be a candidate animal for studies on salivary gland TJ function.
Wed Aug 01 00:00:00 EDT 2018
Mechanical load increase-induced changes in cytoskeletal structure and cellular barrier function in human cerebral endothelial cells.
Biotechnology and bioengineering
Kim D,Kwon S
40-2200 was used in Immunocytochemistry-immunofluorescence to indicate that increased mechanical load due to the applied hypergravity affects the cytoskeletal arrangement and tight junctions, thereby weakening the cell barrier function and enhancing the permeability of the paracellular pathway.
Mon Oct 01 00:00:00 EDT 2018
Characterization of cell-cell junction changes associated with the formation of a strong endothelial barrier.
Tissue barriers
McRae M,LaFratta LM,Nguyen BM,Paris JJ,Hauser KF,Conway DE
40-2200 was used in Immunocytochemistry-immunoflourescence to determine how cell-cell junctions change during endothelial barrier enhancement.
Tue Jan 02 00:00:00 EST 2018
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.
Scientific reports
Lewis PL,Su J,Yan M,Meng F,Glaser SS,Alpini GD,Green RM,Sosa-Pineda B,Shah RN
40-2200 was used in Immunohistochemistry to show phenotypic stability via immunostaining for specific cholangiocyte markers.
Wed Aug 15 00:00:00 EDT 2018
Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity.
Cell research
Xu B,Zhang Y,Du XF,Li J,Zi HX,Bu JW,Yan Y,Han H,Du JL
40-2200 was used in Western Blotting to indicate that miR-132 acts as an intercellular signal mediating neural regulation of the brain vascular integrity.
Sat Jul 01 00:00:00 EDT 2017
Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions.
Nature protocols
Bao X,Lian X,Qian T,Bhute VJ,Han T,Palecek SP
40-2200 was used in Immunocytochemistry to describe fully defined conditions in which human pluripotent stem cells can be differentiated into self-renewing epicardial cells.
Fri Sep 01 00:00:00 EDT 2017
Matrix stiffening promotes a tumor vasculature phenotype.
Proceedings of the National Academy of Sciences of the United States of America
Bordeleau F,Mason BN,Lollis EM,Mazzola M,Zanotelli MR,Somasegar S,Califano JP,Montague C,LaValley DJ,Huynh J,Mencia-Trinchant N,Negrón Abril YL,Hassane DC,Bonassar LJ,Butcher JT,Weiss RS,Reinhart-King CA
402200 was used in immunocytochemistry to evaluate the effects of matrix stiffness on vessel growth and integrity during angiogenesis
Tue Jan 17 00:00:00 EST 2017
Development of a zebrafish sepsis model for high-throughput drug discovery.
Molecular medicine (Cambridge, Mass.)
Philip AM,Wang Y,Mauro A,El-Rass S,Marshall JC,Lee WL,Slutsky AS,dosSantos CC,Wen XY
40-2200 was used in Immunohistochemistry to create a zebrafish sepsis model to further understand the associated pathologies of the whole organisms and validate compounds that can modify this sepsis pathology.
Sat Jul 01 00:00:00 EDT 2017
Effects of HIV-1 Tat and Methamphetamine on Blood-Brain Barrier Integrity and Function In Vitro.
Antimicrobial agents and chemotherapy
Patel S,Leibrand CR,Palasuberniam P,Couraud PO,Weksler B,Jahr FM,McClay JL,Hauser KF,McRae M
40-2200 was used in Western Blotting to indicate that Tat and methamphetamine alter aspects of BBB integrity without affecting net flux of paracellular compounds.
Fri Dec 01 00:00:00 EST 2017
Storage of Porcine Cornea in an Innovative Bioreactor.
Investigative ophthalmology & visual science
Guindolet D,Crouzet E,He Z,Herbepin P,Jumelle C,Perrache C,Dumollard JM,Forest F,Peoc'h M,Gain P,Gabison E,Thuret G
40-2200 was used in Immunohistochemistry to present a novel bioreactor which creates a near physiological environment for the storage of porcine corneas, capable of preserving their main epithelial, limbal, and endothelial characteristics.
Wed Nov 01 00:00:00 EDT 2017
The formation of a functional retinal pigment epithelium occurs on porous polytetrafluoroethylene substrates independently of the surface chemistry.
Journal of materials science. Materials in medicine
Kearns VR,Tasker J,Akhtar R,Bachhuka A,Vasilev K,Sheridan CM,Williams RL
40-2200 was used in Immunocytochemistry to determine whether a functioning monolayer of retinal pigment epithelial cells could be produced on expanded polytetrafluoroethylene substrates modified by either an ammonia plasma treatment of an n-Heptylamine coating.
Tue Aug 01 00:00:00 EDT 2017
Chronic sleep restriction disrupts interendothelial junctions in the hippocampus and increases blood-brain barrier permeability.
Journal of microscopy
Hurtado-Alvarado G,Velázquez-Moctezuma J,Gómez-González B
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to demonstrate that chronic sleep loss disrupts interendothelial junctions that leads to blood-brain barrier hyperpermeability in the hippocampus.
Sun Oct 01 00:00:00 EDT 2017
Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers.
Scientific reports
Klingeborn M,Dismuke WM,Skiba NP,Kelly U,Stamer WD,Bowes Rickman C
40-2200 was used in Immunocytochemistry to characterise the polarised proteomes and physical characteristics of extracellular vesicles released from the retinal pigmented epithelium.
Fri Jul 07 00:00:00 EDT 2017
Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE.
eLife
Li Y,Zhang Y,Xu Y,Kittredge A,Ward N,Chen S,Tsang SH,Yang T
40-2200 was used in Immunocytochemistry to analyse two distinct BEST1 patient mutations, and discover mechanistic correlations between patient clinical phenotypes, electrophysiology in their RPEs, and the structure and function of BESTROPHIN1 mutant channels.
Tue Oct 24 00:00:00 EDT 2017
The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice.
The Journal of biological chemistry
Pelz L,Purfürst B,Rathjen FG
40-2200 was used in Immunohistochemistry to indicate that BT-IgSF is expressed at the BTB and is required for male fertility by supporting the functional integrity of the BTB.
Fri Dec 29 00:00:00 EST 2017
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
eLife
Connell M,Chen H,Jiang J,Kuan CW,Fotovati A,Chu TL,He Z,Lengyell TC,Li H,Kroll T,Li AM,Goldowitz D,Frappart L,Ploubidou A,Patel MS,Pilarski LM,Simpson EM,Lange PF,Allan DW,Maxwell CA
40-2200 was used in Immunocytochemistry to identify an essential role for HMMR in the PLK1-dependent regulatory pathway that orients progenitor cell division and supports neural development.
Tue Oct 10 00:00:00 EDT 2017
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
eLife
Connell M,Chen H,Jiang J,Kuan CW,Fotovati A,Chu TL,He Z,Lengyell TC,Li H,Kroll T,Li AM,Goldowitz D,Frappart L,Ploubidou A,Patel MS,Pilarski LM,Simpson EM,Lange PF,Allan DW,Maxwell CA
40-2200 was used in Immunocytochemistry to identify an essential role for HMMR in the PLK1-dependent regulatory pathway that orients progenitor cell division and supports neural development.
Tue Oct 10 00:00:00 EDT 2017
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
eLife
Connell M,Chen H,Jiang J,Kuan CW,Fotovati A,Chu TL,He Z,Lengyell TC,Li H,Kroll T,Li AM,Goldowitz D,Frappart L,Ploubidou A,Patel MS,Pilarski LM,Simpson EM,Lange PF,Allan DW,Maxwell CA
40-2200 was used in Immunocytochemistry to identify an essential role for HMMR in the PLK1-dependent regulatory pathway that orients progenitor cell division and supports neural development.
Tue Oct 10 00:00:00 EDT 2017
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
eLife
Connell M,Chen H,Jiang J,Kuan CW,Fotovati A,Chu TL,He Z,Lengyell TC,Li H,Kroll T,Li AM,Goldowitz D,Frappart L,Ploubidou A,Patel MS,Pilarski LM,Simpson EM,Lange PF,Allan DW,Maxwell CA
40-2200 was used in Immunocytochemistry to identify an essential role for HMMR in the PLK1-dependent regulatory pathway that orients progenitor cell division and supports neural development.
Tue Oct 10 00:00:00 EDT 2017
Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice.
Bio-protocol
Assmann JC,Müller K,Wenzel J,Walther T,Brands J,Thornton P,Allan SM,Schwaninger M
40-2200 was used in Immunocytochemistry to show isolation of adult mice brain endothelial cells.
Sat May 20 00:00:00 EDT 2017
Proteomic characterization of epicardial-myocardial signaling reveals novel regulatory networks including a role for NF-κB in epicardial EMT.
PloS one
Li Y,Urban A,Midura D,Simon HG,Wang QT
402200 was used in immunocytochemistry to report the secretome of embryonic chicken EPDC-heart explant co-cultures
Mon Aug 28 00:00:00 EDT 2017
Glutaminolysis is Essential for Energy Production and Ion Transport in Human Corneal Endothelium.
EBioMedicine
Zhang W,Li H,Ogando DG,Li S,Feng M,Price FW,Tennessen JM,Bonanno JA
40-2200 was used in immunohistochemistry - paraffin section to study factors that regulate metabolism in the corneal endothelium
Wed Feb 01 00:00:00 EST 2017
A new strategy to measure intercellular adhesion forces in mature cell-cell contacts.
Scientific reports
Sancho A,Vandersmissen I,Craps S,Luttun A,Groll J
40-2200 was used in Immunocytochemistry to reveal how intercellular adhesion forces in monolayer decrease significantly at an early stage of EndMT and we show that cells undergo stiffening and flattening at this stage.
Mon Apr 10 00:00:00 EDT 2017
Role of Nrf2 and protective effects of Metformin against tobacco smoke-induced cerebrovascular toxicity.
Redox biology
Prasad S,Sajja RK,Kaisar MA,Park JH,Villalba H,Liles T,Abbruscato T,Cucullo L
402200 was used in immunocytochemistry and western blot to investigate the role of nuclear factor erythroid 2-related factor in cigarette smoking-induced cerebrobvascular/blood brain barrier impairments
Tue Aug 01 00:00:00 EDT 2017
The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis through decreased glutathione and SOX17.
Hepatology (Baltimore, Md.)
Waisbourd-Zinman O,Koh H,Tsai S,Lavrut PM,Dang C,Zhao X,Pack M,Cave J,Hawes M,Koo KA,Porter JR,Wells RG
40-2200 was used in immunocytochemistry to study a decrease in glutathione and SOX17 and toxin biliastresone that causes mouse extrahepatic cholangiocyte damage and fibrosis
Thu Sep 01 00:00:00 EDT 2016
The endogenous cannabinoid anandamide increases human airway epithelial cell permeability through an arachidonic acid metabolite.
Pharmacological research
Shang VC,O'Sullivan SE,Kendall DA,Roberts RE
40-2200 was used in western blot to research an increase in human airway epithelial cell permeability through an arachidonic acid metabolite caused by the endogenous cannabinoid anandamide
Tue Mar 01 00:00:00 EST 2016
CPEB1 mediates epithelial-to-mesenchyme transition and breast cancer metastasis.
Oncogene
Nagaoka K,Fujii K,Zhang H,Usuda K,Watanabe G,Ivshina M,Richter JD
40-2200 was used in western blot to test if ZO-1-mediated diminution of polarity results in an epithelial-to-mesenchyme transition and increased metastatic potential
Thu Jun 02 00:00:00 EDT 2016
Role of Hypoxia Inducible Factor 1 in Hyperglycemia-Exacerbated Blood-Brain Barrier Disruption in Ischemic Stroke.
Neurobiology of disease
Zhang Z,Yan J,Shi H
40-2200 was used in Western Blotting to test a hypothesis that the activation of hypoxia inducible factor-1 (HIF-1) was involved in hyperglycemia-aggravated BBB disruption in an ischemic stroke model.
Tue Nov 01 00:00:00 EDT 2016
Δ9-Tetrahydrocannabinol reverses TNFα-induced increase in airway epithelial cell permeability through CB2 receptors.
Biochemical pharmacology
Shang VC,Kendall DA,Roberts RE
402200 was used in western blot to investigate the effects of tetrahydrocannabinol on bronchial epithelial cell permeability after exposure to TNF-alpha
Tue Nov 15 00:00:00 EST 2016
An in vitro model of murine middle ear epithelium.
Disease models & mechanisms
Mulay A,Akram KM,Williams D,Armes H,Russell C,Hood D,Armstrong S,Stewart JP,Brown SD,Bingle L,Bingle CD
402200 was used in immunocytochemistry to design a model of mouse middle ear epithelial cells that recapitulates the characteristics of the native murine middle ear epithelium
Tue Nov 01 00:00:00 EDT 2016
High glucose-induced epithelial-mesenchymal transition contributes to the upregulation of fibrogenic factors in retinal pigment epithelial cells.
International journal of molecular medicine
Che D,Zhou T,Lan Y,Xie J,Gong H,Li C,Feng J,Hong H,Qi W,Ma C,Wu Q,Yang X,Gao G
402200 was used in western blot to determine the effects of high glucose on the epithelial-mesenchymal transition in retinal pigment epithelial cells
Thu Dec 01 00:00:00 EST 2016
NLRP3 inflammasome activation drives bystander cone photoreceptor cell death in a P23H rhodopsin model of retinal degeneration.
Human molecular genetics
Viringipurampeer IA,Metcalfe AL,Bashar AE,Sivak O,Yanai A,Mohammadi Z,Moritz OL,Gregory-Evans CY,Gregory-Evans K
40-2200 was used in immunohistochemistry to assess the drive of bystander cone photoreceptor cell death in a P23H rhodopsin model of retinal degeneration by NLRP3 inflammasome activation
Fri Apr 15 00:00:00 EDT 2016
GATA4 Regulates Blood-Testis Barrier Function and Lactate Metabolism in Mouse Sertoli Cells.
Endocrinology
Schrade A,Kyrönlahti A,Akinrinade O,Pihlajoki M,Fischer S,Rodriguez VM,Otte K,Velagapudi V,Toppari J,Wilson DB,Heikinheimo M
40-2200 was used in immunocytochemistry determine the effects of Gata4 depletion in Sertoli cells
Wed Jun 01 00:00:00 EDT 2016
Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts.
Investigative ophthalmology & visual science
He Z,Forest F,Bernard A,Gauthier AS,Montard R,Peoc'h M,Jumelle C,Courrier E,Perrache C,Gain P,Thuret G
402200 was used in immunocytochemistry to develop a process to obtain appropriate carriers by recycling human corneas unsuitable for graft in their original state, but liable to provide multiple thin lamellae when cut with a femtosecond laser as used in refractive surgery
Thu Dec 01 00:00:00 EST 2016
Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer.
American journal of physiology. Gastrointestinal and liver physiology
Vorvis C,Hatziapostolou M,Mahurkar-Joshi S,Koutsioumpa M,Williams J,Donahue TR,Poultsides GA,Eibl G,Iliopoulos D
40-2200 was used in immunohistochemistry identify transcription factors involved in pancreatic ductal adenocarcinoma pathogenesis
Wed Jun 01 00:00:00 EDT 2016
Leukocyte Infiltration Triggers Seizure Recurrence in a Rat Model of Temporal Lobe Epilepsy.
Inflammation
Liu Z,Wang S,Liu J,Wang F,Liu Y,Zhao Y
40-2200 was used in western blot to characterize infiltration by leukocytes that trigger seizure recurrence in a rat model of temporal lobe epilepsy
Wed Jun 01 00:00:00 EDT 2016
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
Methods (San Diego, Calif.)
Stebbins MJ,Wilson HK,Canfield SG,Qian T,Palecek SP,Shusta EV
40-2200 was used in immunocytochemistry to describe methods to differentiate and characterize hPSC-derived brain microvascular endothelial cells
Sun May 15 00:00:00 EDT 2016
A Novel Peptide Hydrogel for an Antimicrobial Bandage Contact Lens.
Advanced healthcare materials
Gallagher AG,Alorabi JA,Wellings DA,Lace R,Horsburgh MJ,Williams RL
40-2200 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to study a peptide hydrogel with an antimicrobial activity is developed as a bandage contact lens.
Mon Aug 01 00:00:00 EDT 2016
Dynamics of BMP and Hes1/Hairy1 signaling in the dorsal neural tube underlies the transition from neural crest to definitive roof plate.
BMC biology
Nitzan E,Avraham O,Kahane N,Ofek S,Kumar D,Kalcheim C
40-2200 was used in immunohistochemistry to study dynamics in the dorsal neural tube and the underlying transition from neural crest to definitive roof plate by BMP and Hes1/Hairy1 signaling
Thu Mar 24 00:00:00 EDT 2016
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration.
Journal of visualized experiments : JoVE
Trembley MA,Velasquez LS,Small EM
40-2200 was used in immunocytochemistry to study ex vivo assessment and epicardial outgrowth culture assay of epicardial-derived cell migration
Fri Mar 18 00:00:00 EDT 2016
Hypertonic saline alleviates experimentally induced cerebral oedema through suppression of vascular endothelial growth factor and its receptor VEGFR2 expression in astrocytes.
BMC neuroscience
Huang L,Cao W,Deng Y,Zhu G,Han Y,Zeng H
402200 was used in western blot to elucidate the mechanisms by which hypertonic saline attenuates cerebral oedema
Thu Oct 13 00:00:00 EDT 2016
Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane.
Nutrition & metabolism
Soayfane Z,Tercé F,Cantiello M,Robenek H,Nauze M,Bézirard V,Allart S,Payré B,Capilla F,Cartier C,Peres C,Al Saati T,Théodorou V,Nelson DW,Yen CE,Collet X,Coméra C
40-2200 was used in immunocytochemistry evaluate the contribution of cytosolic lipid droplets to lipid absorption
Mon Aug 01 00:00:00 EDT 2016
Arhgap17, a RhoGTPase activating protein, regulates mucosal and epithelial barrier function in the mouse colon.
Scientific reports
Lee SY,Kim H,Kim K,Lee H,Lee S,Lee D
40-2200 was used in immunohistochemistry study the intestinal barrier function using Arhgap17-deficient mice
Fri May 27 00:00:00 EDT 2016
Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses.
Virology journal
Ehlen L,Tödtmann J,Specht S,Kallies R,Papies J,Müller MA,Junglen S,Drosten C,Eckerle I
40-2200 was used in immunocytochemistry to research the susceptibility of epithelial cell lines of the cotton rat (Sigmodon hispidus) in vitro models to zoonotic Rhabdo-, Bunya-, and Flaviviruses
Wed May 04 00:00:00 EDT 2016
Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.
Scientific reports
Xiong J,Zhou MF,Wang YD,Chen LP,Xu WF,Wang YD,Deng F,Liu SD
40-2200 was used in western blot test if protein kinase D2 affects murine colitis
Fri Sep 23 00:00:00 EDT 2016
In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart.
Journal of visualized experiments : JoVE
Ramesh S,Singh A,Cibi DM,Hausenloy DJ,Singh MK
40-2200 was used in immunocytochemistry to elucidate mouse embryonic heart from an in vitro culture of epicardial cells
Wed Apr 27 00:00:00 EDT 2016
Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa.
The American journal of clinical nutrition
Goichon A,Bertrand J,Chan P,Lecleire S,Coquard A,Cailleux AF,Vaudry D,Déchelotte P,Coëffier M
40-2200 was used in western blot to examine what proteins are produced when carbohydrate-fed healthy humans receive enteral delivery of proteins.
Sat Aug 01 00:00:00 EDT 2015
Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Watari A,Hashegawa M,Yagi K,Kondoh M
40-2200 was used in immunocytochemistry and western blot to investigate the effect of homoharringtonine on intestinal epithelial permeability and its mechanism
Thu Jan 01 00:00:00 EST 2015
Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells.
Journal of cell science
Cong X,Zhang Y,Li J,Mei M,Ding C,Xiang RL,Zhang LW,Wang Y,Wu LL,Yu GY
40-2200 was used in immunocytochemistry to propose that claudin-4 is required for mAChR-modulated paracellular permeability of epithelial cells and investigate the mechanism
Mon Jun 15 00:00:00 EDT 2015
17β-estradiol inhibits MMP-9 and SUR1/TrpM4 expression and activation and thereby attenuates BSCB disruption/hemorrhage after spinal cord injury in male rats.
Endocrinology
Lee JY,Choi HY,Na WH,Ju BG,Yune TY
40-2200 was used in western blot to determine the effect of 17beta-estradiol on blood-spinal cord barrier breakdown, hemorrhage, and subsequent inflammation after spinal cord injury
Fri May 01 00:00:00 EDT 2015
Effect of differentiation on endocytic profiles of endothelial and epithelial cell culture models.
Experimental cell research
Ilina P,Partti S,Niklander J,Ruponen M,Lou YR,Yliperttula M
40-2200 was used in immunocytochemistry to investigate the changes in endocytosis during differentiation
Sun Mar 01 00:00:00 EST 2015
Gold Nanoparticles Increase Endothelial Paracellular Permeability by Altering Components of Endothelial Tight Junctions, and Increase Blood-Brain Barrier Permeability in Mice.
Toxicological sciences : an official journal of the Society of Toxicology
Li CH,Shyu MK,Jhan C,Cheng YW,Tsai CH,Liu CW,Lee CC,Chen RM,Kang JJ
40-2200 was used in immunocytochemistry to assess the effects of treatment with gold nanoparticles on human cells
Sun Nov 01 00:00:00 EDT 2015
Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum.
American journal of physiology. Renal physiology
Liu KM,Chuang SM,Long CY,Lee YL,Wang CC,Lu MC,Lin RJ,Lu JH,Jang MY,Wu WJ,Ho WT,Juan YS
40-2200 was used in western blot to elucidate ketamine-induced ulcerative cystitis and bladder apoptosis in association with oxidative stress mediated by mitochondria and the endoplasmic reticulum.
Sat Aug 15 00:00:00 EDT 2015
MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells.
Oncogene
Formosa A,Markert EK,Lena AM,Italiano D,Finazzi-Agro' E,Levine AJ,Bernardini S,Garabadgiu AV,Melino G,Candi E
40-2200 was used in western blot to identify miRNAs that contribute to prostate cancer.
Thu Oct 30 00:00:00 EDT 2014
MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney.
The American journal of pathology
Ihara K,Asanuma K,Fukuda T,Ohwada S,Yoshida M,Nishimori K
40-2200 was used in immunohistochemistry - frozen section to explore the role of MAGI-2 in the murine kidney
Wed Oct 01 00:00:00 EDT 2014
Mast cells protect against Pseudomonas aeruginosa-induced lung injury.
The American journal of pathology
Junkins RD,Carrigan SO,Wu Z,Stadnyk AW,Cowley E,Issekutz T,Berman J,Lin TJ
40-2200 was used in immunocytochemistry and western blot to determine if mast cells regulate airway epithelial integrity using a P. aeruginosa lung infection mouse model.
Fri Aug 01 00:00:00 EDT 2014
Delayed near-infrared analysis permits visualization of rodent retinal pigment epithelium layer in vivo.
Journal of biomedical optics
Pankova N,Zhao X,Liang H,Baek DS,Wang H,Boyd S
40-2200 was used in immunocytochemistry to describe a method to visualize rat retinal pigment epithelium.
Fri Feb 20 00:00:00 EST 2015
Cranial irradiation alters the brain's microenvironment and permits CCR2+ macrophage infiltration.
PloS one
Morganti JM,Jopson TD,Liu S,Gupta N,Rosi S
40-2200 was used in western blot to
Mon Feb 02 00:00:00 EST 2015
Corneal endothelial cell fate is maintained by LGR5 through the regulation of hedgehog and Wnt pathway.
Stem cells (Dayton, Ohio)
Hirata-Tominaga K,Nakamura T,Okumura N,Kawasaki S,Kay EP,Barrandon Y,Koizumi N,Kinoshita S
40-2200 was used in immunohistochemistry to examine the role of LGR5 in human corneal tissue.
Mon Jul 01 00:00:00 EDT 2013
A2E accumulation influences retinal microglial activation and complement regulation.
Neurobiology of aging
Ma W,Coon S,Zhao L,Fariss RN,Wong WT
40-2200 was used in immunohistochemistry to test if accumulation of A2E alters retinal microglia in pathologically relevant ways.
Fri Mar 01 00:00:00 EST 2013
Histopathological, immunohistochemical criteria and confocal laser-scanning data of arthrofibrosis.
Pathology, research and practice
Ruppert M,Theiss C,Knöß P,Kendoff D,Krukemeyer MG,Schröder N,Brand-Saberi B,Gehrke T,Krenn V
40-2200 was used in immunohistochemistry to describe the histopathological substrate of arthrofibrosis.
Fri Nov 01 00:00:00 EDT 2013
Stromal Claudin14-heterozygosity, but not deletion, increases tumour blood leakage without affecting tumour growth.
PloS one
Baker M,Reynolds LE,Robinson SD,Lees DM,Parsons M,Elia G,Hodivala-Dilke K
40-2200 was used in immunohistochemistry - frozen section to determine if Claudin 4 regulates tumor blood vessel leakage, angiogenesis, and tumor growth.
Mon Dec 09 00:00:00 EST 2013
HIF-1 is involved in high glucose-induced paracellular permeability of brain endothelial cells.
Cellular and molecular life sciences : CMLS
Yan J,Zhang Z,Shi H
40-2200 was used in western blot to determine the role of hypoxia-inducible factor-1 in high glucose-induced endothelial permeability of the blood brain barrier.
Sun Jan 01 00:00:00 EST 2012
Long-term safety and efficacy of human-induced pluripotent stem cell (iPS) grafts in a preclinical model of retinitis pigmentosa.
Molecular medicine (Cambridge, Mass.)
Li Y,Tsai YT,Hsu CW,Erol D,Yang J,Wu WH,Davis RJ,Egli D,Tsang SH
40-2200 was used in immunocytochemistry to study the efficacy of induced pluripotent stem transplantation in restoring functional vision in Rpe65(rd2)/Rpe65(rd2) mice.
Thu Dec 06 00:00:00 EST 2012
Extracellular matrix assembly and 3D organization during paraxial mesoderm development in the chick embryo.
Developmental biology
Rifes P,Thorsteinsdóttir S
40-2200 was used in immunohistochemistry to assess the distribution of fibronectin and laminin during development of the chick paraxial mesoderm.
Wed Aug 15 00:00:00 EDT 2012
Redistribution of adhering junctions in human endometrial epithelial cells during the implantation window of the menstrual cycle.
Histochemistry and cell biology
Buck VU,Windoffer R,Leube RE,Classen-Linke I
40-2200 was used in immunohistochemistry - frozen section to assess cell-cell adhesion throughout the menstrual cycle.
Fri Jun 01 00:00:00 EDT 2012
Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.
Journal of allergy
Stewart CE,Torr EE,Mohd Jamili NH,Bosquillon C,Sayers I
40-2200 was used in immunocytochemistry to assess primary and non-primary bronchial epithelial cell culture systems as models for asthma research
Thu Aug 23 00:00:00 EDT 2012
Distinct distribution of the tensin family in the mouse kidney and small intestine.
Experimental animals
Nishino T,Sasaki N,Chihara M,Nagasaki K,Torigoe D,Kon Y,Agui T
40-2200 was used in immunohistochemistry to determine and compare the cellular and subcellular distributions of tensin family members.
Thu Apr 11 00:00:00 EDT 2013
Interactions between β-catenin and transforming growth factor-β signaling pathways mediate epithelial-mesenchymal transition and are dependent on the transcriptional co-activator cAMP-response element-binding protein (CREB)-binding protein (CBP).
The Journal of biological chemistry
Zhou B,Liu Y,Kahn M,Ann DK,Han A,Wang H,Nguyen C,Flodby P,Zhong Q,Krishnaveni MS,Liebler JM,Minoo P,Crandall ED,Borok Z
40-2200 was used in Western Blotting to identify a new therapeutic approach to pulmonary fibrosis by specifically uncoupling cAMP-response element-binding protein/catenin-dependent signalling downstream of transforming growth factor-Β.
Fri Mar 02 00:00:00 EST 2012
CD40-activated B cells can efficiently prime antigen-specific naïve CD8+ T cells to generate effector but not memory T cells.
PloS one
Mathieu M,Cotta-Grand N,Daudelin JF,Boulet S,Lapointe R,Labrecque N
40-2200 was used in flow cytometry to test the ability of different types of antigen presenting cells to induce a CD8(+) T cell response.
Mon Jun 04 00:00:00 EDT 2012
Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm.
Development (Cambridge, England)
Loebel DA,Studdert JB,Power M,Radziewic T,Jones V,Coultas L,Jackson Y,Rao RS,Steiner K,Fossat N,Robb L,Tam PP
40-2200 was used in immunohistochemistry - frozen section to study the role of Rhou in development.
Sat Oct 01 00:00:00 EDT 2011
LGN-dependent orientation of cell divisions in the dermomyotome controls lineage segregation into muscle and dermis.
Development (Cambridge, England)
Ben-Yair R,Kahane N,Kalcheim C
40-2200 was used in immunohistochemistry - paraffin section to study how the plane of cell division affects differential fate acquisition.
Sat Oct 01 00:00:00 EDT 2011
The fibronectin leucine-rich repeat transmembrane protein Flrt2 is required in the epicardium to promote heart morphogenesis.
Development (Cambridge, England)
Müller PS,Schulz R,Maretto S,Costello I,Srinivas S,Bikoff E,Robertson E
40-2200 was used in immunohistochemistry to report that Flrt2 and Flrt3 are functionally interchangeable in the developing epicardium.
Fri Apr 01 00:00:00 EDT 2011
N-methyl-D-aspartate receptor subunit NR3a expression and function in principal cells of the collecting duct.
American journal of physiology. Renal physiology
Sproul A,Steele SL,Thai TL,Yu S,Klein JD,Sands JM,Bell PD
40-2200 was used in immunocytochemistry to determine the role of NR3a in the kidney using knockout mice.
Fri Jul 01 00:00:00 EDT 2011
Secondary neurulation: Fate-mapping and gene manipulation of the neural tube in tail bud.
Development, growth & differentiation
Shimokita E,Takahashi Y
40-2200 was used in immunohistochemistry to identify the origin of secondary neurulation-undergoing cells.
Fri Apr 01 00:00:00 EDT 2011
Dynamics of claudins expression in colitis and colitis-associated cancer in rat.
Methods in molecular biology (Clifton, N.J.)
Arimura Y,Nagaishi K,Hosokawa M
Fri Nov 04 00:00:00 EDT 2011
Dynamics of claudins expression in colitis and colitis-associated cancer in rat.
Methods in molecular biology (Clifton, N.J.)
Arimura Y,Nagaishi K,Hosokawa M
Fri Nov 04 00:00:00 EDT 2011
MicroRNA-processing enzyme Dicer is required in epicardium for coronary vasculature development.
The Journal of biological chemistry
Singh MK,Lu MM,Massera D,Epstein JA
40-2200 was used in immunohistochemistry to study the role of miRNAs during epicardial development.
Fri Nov 25 00:00:00 EST 2011
The Rho GTPase Cdc42 is required for primary mammary epithelial cell morphogenesis in vitro.
Small GTPases
Bray K,Brakebusch C,Vargo-Gogola T
40-2200 was used in immunocytochemistry to elucidate the role of Cdc42 during normal mammary epithelial cell morphogenesis.
Thu Sep 01 00:00:00 EDT 2011
Primary cilia regulate mTORC1 activity and cell size through Lkb1.
Nature cell biology
Boehlke C,Kotsis F,Patel V,Braeg S,Voelker H,Bredt S,Beyer T,Janusch H,Hamann C,Gödel M,Müller K,Herbst M,Hornung M,Doerken M,Köttgen M,Nitschke R,Igarashi P,Walz G,Kuehn EW
40-2200 was used in Immunocytochemistry to demonstrate that the cilium regulates mTOR signalling and cell size, and identify the cilium-basal body compartment as a spatially restricted activation site for Lkb1 signalling.
Mon Nov 01 00:00:00 EDT 2010
Microscopic mammalian retinal pigment epithelium lesions induce widespread proliferation with differences in magnitude between center and periphery.
Molecular vision
von Leithner PL,Ciurtin C,Jeffery G
40-2200 was used in immunohistochemistry to test if peripheral retinal pigment epithelium is fundamentally different from central retinal pigment epithelium in terms of proliferation and regeneration
Wed Mar 31 00:00:00 EDT 2010
RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
Nature cell biology
Nakaya Y,Sukowati EW,Wu Y,Sheng G
40-2200 was used in immunohistochemistry to study the epithelial-mesenchymal transition during gastrulation
Tue Jul 01 00:00:00 EDT 2008
Gap junction remodelling in human heart failure is associated with increased interaction of connexin43 with ZO-1.
Cardiovascular research
Bruce AF,Rothery S,Dupont E,Severs NJ
40-2200 was used in Immunohistochemistry (Frozen) to show failing heart in human is associated with increased co-localization of connexin43 with ZO-1.
Sat Mar 01 00:00:00 EST 2008
ZO-1 is required for protein kinase C gamma-driven disassembly of connexin 43.
Cellular signalling
Akoyev V,Takemoto DJ
40-2200 was used in immunocytochemistry and western blot to determine the specific role of zonula occludens protein-1 in PKC-gamma-driven connexin43 disassembly
Tue May 01 00:00:00 EDT 2007
Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium.
The Journal of comparative neurology
Daniele LL,Adams RH,Durante DE,Pugh EN,Philp NJ
40-2200 was used in immunohistochemistry to localize junctional adhesion molecule-C in the neural retina and retinal pigment epithelium
Sat Nov 10 00:00:00 EST 2007
Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.
The European journal of neuroscience
Penes MC,Li X,Nagy JI
Fri Jul 01 00:00:00 EDT 2005
