Invitrogen

ZO-1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

11 References

View all (43) ZO-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

ZO-1 Antibody (PA5-85256) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ZO-1 was performed in NT2D1 cells fixed in ice-cold MeOH for 5 min. Green: ZO-1 Polyclonal Antibody (Product # PA5-85256) diluted at 1:200. Blue: Hoechst 33342 staining.

ZO-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ZO-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ZO-1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ZO-1 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. HeLa cells were transfected with ZO-1 shRNA and decrease in signal intensity was observed in Western blotting using Anti-ZO-1 Polyclonal Antibody (Product # PA5-85256). {KD}

Product Details

PA5-85256

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

- -

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.12 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792399

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (95%), Rat (94%), Dog (95%).

Positive Control: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, mouse testis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long Noncoding RNA ENSG00000254693 Promotes Diabetic Kidney Disease via Interacting with HuR.

Journal of diabetes research

Yu Q,Lin J,Ma Q,Li Y,Wang Q,Chen H,Liu Y,Liu B

Published figure using ZO-1 polyclonal antibody (Product # PA5-85256) in Immunocytochemistry

Tue May 03 00:00:00 EDT 2022

Long Noncoding RNA ENSG00000254693 Promotes Diabetic Kidney Disease via Interacting with HuR.

Journal of diabetes research

Yu Q,Lin J,Ma Q,Li Y,Wang Q,Chen H,Liu Y,Liu B

Published figure using ZO-1 polyclonal antibody (Product # PA5-85256) in Immunocytochemistry

Tue May 03 00:00:00 EDT 2022

The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis.

Frontiers in microbiology

Ma X,Jin H,Chu X,Dai W,Tang W,Zhu J,Wang F,Yang X,Li W,Liu G,Yang X,Liang H

PA5-85256 was used in Immunohistochemistry to reveal the unexpected function of host CYP1A1 in microbiota-mediated cadaverine metabolism, with crucial consequences for dysbacteriosis following MRSA-induced abdominal sepsis, indicating that inhibiting CYP1A1 or blocking cadaverine-histamine H4 receptor signaling could be a potential therapeutic target against abdominal sepsis.

Sat Jul 16 00:00:00 EDT 2022

The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis.

Frontiers in microbiology

Ma X,Jin H,Chu X,Dai W,Tang W,Zhu J,Wang F,Yang X,Li W,Liu G,Yang X,Liang H

Sat Jul 16 00:00:00 EDT 2022

Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease.

Journal of neuroinflammation

Qian XH,Liu XL,Chen G,Chen SD,Tang HD

PA5-85256 was used in Immunohistochemistry and Western Blotting to suggest that Aβ in the CNS can induce gut microbiota dysbiosis, alter intestinal structure and accelerate the amyloidogenic pathways, which were related to inhibiting cholinergic anti-inflammatory pathways.

Wed Sep 28 00:00:00 EDT 2022

Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease.

Journal of neuroinflammation

Qian XH,Liu XL,Chen G,Chen SD,Tang HD

Wed Sep 28 00:00:00 EDT 2022

Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease.

Journal of neuroinflammation

Qian XH,Liu XL,Chen G,Chen SD,Tang HD

Wed Sep 28 00:00:00 EDT 2022

Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease.

Journal of neuroinflammation

Qian XH,Liu XL,Chen G,Chen SD,Tang HD

Wed Sep 28 00:00:00 EDT 2022

Transmissible Gastroenteritis Virus Infection Promotes the Self-Renewal of Porcine Intestinal Stem Cells via Wnt/β-Catenin Pathway.

Journal of virology

Yang N,Zhang Y,Fu Y,Li Y,Yang S,Chen J,Liu G

PA5-85256 was used in Immunohistochemistry to investigate he mechanism behind the epithelium regeneration upon ransmissible gastroenteritis virus infection.

Wed Sep 28 00:00:00 EDT 2022

Overexpression of Mitochondrial Ferritin Enhances Blood-Brain Barrier Integrity Following Ischemic Stroke in Mice by Maintaining Iron Homeostasis in Endothelial Cells.

Antioxidants (Basel, Switzerland)

Wang P,Ren Q,Shi M,Liu Y,Bai H,Chang YZ

PA5-85256 was used in Western Blotting to identify a previously unexplored effect for FtMt in the BBB and provide evidence that iron-mediated oxidative stress in BMVECs is an early cause of BMVECs damage and BBB breakdown in ischemic stroke.

Sun Jun 26 00:00:00 EDT 2022

Overexpression of Mitochondrial Ferritin Enhances Blood-Brain Barrier Integrity Following Ischemic Stroke in Mice by Maintaining Iron Homeostasis in Endothelial Cells.

Antioxidants (Basel, Switzerland)

Wang P,Ren Q,Shi M,Liu Y,Bai H,Chang YZ

Sun Jun 26 00:00:00 EDT 2022

Ursodeoxycholic Acid Treatment Restores Gut Microbiota and Alleviates Liver Inflammation in Non-Alcoholic Steatohepatitic Mouse Model.

Frontiers in pharmacology

Li H,Wang Q,Chen P,Zhou C,Zhang X,Chen L

PA5-85256 was used in Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to explore the dynamic changes of gut microbiota at the different stages of NAFLD and determine whether ursodeoxycholic acid (UDCA) could improve liver histopathological features of non-alcoholic steatohepatitis (NASH) mice induced by a high-fat high-cholesterol (HFHC) diet and its impact on gut microbiota.

Fri Dec 24 00:00:00 EST 2021

Ursodeoxycholic Acid Treatment Restores Gut Microbiota and Alleviates Liver Inflammation in Non-Alcoholic Steatohepatitic Mouse Model.

Frontiers in pharmacology

Li H,Wang Q,Chen P,Zhou C,Zhang X,Chen L

Fri Dec 24 00:00:00 EST 2021

N-(3',4'-dimethoxycinnamonyl) anthranilic acid alleviates severe acute pancreatitis by inhibiting intestinal barrier dysfunction and NF-κB activation.

Drug development research

Zhao Z,Lu L,Li W

PA5-85256 was used in Western Blotting to investigate the role of anthranilic acid active synthetic derivative, N-(3',4'-dimethoxycinnamonyl) anthranilic acid (3,4-DAA), in intestinal barrier dysfunction of SAP.

Sat May 01 00:00:00 EDT 2021

N-(3',4'-dimethoxycinnamonyl) anthranilic acid alleviates severe acute pancreatitis by inhibiting intestinal barrier dysfunction and NF-κB activation.

Drug development research

Zhao Z,Lu L,Li W

Sat May 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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Gene Aliases: TJP1; ZO-1; ZO1

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UniProt ID: (Human) Q07157, (Mouse) P39447

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Entrez Gene ID: (Human) 7082, (Mouse) 21872

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Function(s)

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion protein C-terminus binding tight junction

Process(es)

blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-1 Polyclonal Antibody

ZO-1 Antibody (PA5-85256) in ICC/IF

Product Details

PA5-85256

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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