Proteintech

ZO-2 Polyclonal Antibody

ZO-2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ZO-2 Antibody (18900-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 18900-1-AP (ZO-2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

18900-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 12 publications 12 publications

Immunohistochemistry (IHC)

Assay-dependent

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZO-2 Fusion Protein Ag13534 (1-289 aa encoded by BC027592)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPVRGDRGF PPRRELSGWL RAPGMEELIW EQYTVTLQKD SKRGFGIAVS GGRDNPHFEN GETSIVISDV LPGGPADGLL QENDRVVMVN GTPMEDVLHS FAVQQLRKSG KVAAIVVKRP RKVQVAALQA SPPLDQDDRA FEVMDEFDGR SFRSGYSERS RLNSHGGRSR SWEDSPERGR PHERARSRER DLSRDRSRGR SLERGLDQDH ARTRDRSRGR SLERGLDHDF GPSRDRDRDR SRGRSIDQDY ERAYHRAYDP DYERAYSPEY RRGARHDARS RGPRSRSREH (1-289 aa encoded by BC027592)

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ZNF582 overexpression restrains the progression of clear cell renal cell carcinoma by enhancing the binding of TJP2 and ERK2 and inhibiting ERK2 phosphorylation.

Cell death & disease

Yang W,Zhang Z,Li L,Zhang K,Xu Y,Xia M,Zhou J,Gong Y,Chen J,Gong K

Sat Mar 25 00:00:00 EDT 2023

ZNF582 overexpression restrains the progression of clear cell renal cell carcinoma by enhancing the binding of TJP2 and ERK2 and inhibiting ERK2 phosphorylation.

Cell death & disease

Yang W,Zhang Z,Li L,Zhang K,Xu Y,Xia M,Zhou J,Gong Y,Chen J,Gong K

Sat Mar 25 00:00:00 EDT 2023

Benzo[a]pyrene induces epithelial tight junction disruption and apoptosis via inhibiting the initiation of autophagy in intestinal porcine epithelial cells.

Chemico-biological interactions

Li J,Bai J,Si X,Jia H,Wu Z

Sat Apr 01 00:00:00 EDT 2023

Gut Microbiota from Short-Chain Chlorinated Paraffin-Exposed Mice Promotes Astrocyte Activation by Disrupting the Intestinal Tight Junction via Zonulin Upregulation.

Journal of agricultural and food chemistry

Zhao X,Zhang T,Zheng Y,Zhao Z,Ding W,Zhang Z,Zhang Z,Wang R,Jiao M,Liu L,Yu S,Wang X,Huang R,Wu Q

Wed May 31 00:00:00 EDT 2023

Gut Microbiota from Short-Chain Chlorinated Paraffin-Exposed Mice Promotes Astrocyte Activation by Disrupting the Intestinal Tight Junction via Zonulin Upregulation.

Journal of agricultural and food chemistry

Zhao X,Zhang T,Zheng Y,Zhao Z,Ding W,Zhang Z,Zhang Z,Wang R,Jiao M,Liu L,Yu S,Wang X,Huang R,Wu Q

Wed May 31 00:00:00 EDT 2023

Oral Delivery of Mouse β-Defensin 14 (mBD14)-Producing Lactococcus lactis NZ9000 Attenuates Experimental Colitis in Mice.

Journal of agricultural and food chemistry

Tian H,Li J,Chen X,Ren Z,Pan X,Huang W,Bhatia M,Pan LL,Sun J

Wed Apr 05 00:00:00 EDT 2023

Qingchang Wenzhong Decoction Prevents the Occurrence of Intestinal Tumors by Regulating Intestinal Microbiota and Gasdermin E.

Frontiers in physiology

Ren L,Zhang Z,Zhao W,Zhao B,Chen X,Wang Y,Chen Z,Ye J,Yang Y,Cao P

Tue Aug 02 00:00:00 EDT 2022

Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice.

Molecular biology reports

Tsurudome Y,Morita N,Horiguchi M,Ushijima K

Thu Dec 01 00:00:00 EST 2022

Morchella esculenta polysaccharide attenuate obesity, inflammation and modulate gut microbiota.

AMB Express

Rehman AU,Khan AI,Xin Y,Liang W

Sat Sep 03 00:00:00 EDT 2022

Improved epithelial cell-cell adhesion using molecular mobility of supramolecular surfaces.

Biomaterials science

Mikami R,Arisaka Y,Hakariya M,Iwata T,Yui N

Tue Oct 26 00:00:00 EDT 2021

The zonula occludens protein family regulates the hepatic barrier system in the murine liver.

Biochimica et biophysica acta. Molecular basis of disease

Itoh M,Terada M,Sugimoto H

Fri Jan 01 00:00:00 EST 2021

The zonula occludens protein family regulates the hepatic barrier system in the murine liver.

Biochimica et biophysica acta. Molecular basis of disease

Itoh M,Terada M,Sugimoto H

Fri Jan 01 00:00:00 EST 2021

Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.

Free radical biology & medicine

Tocmo R,Le B,Heun A,van Pijkeren JP,Parkin K,Johnson JJ

Mon Feb 01 00:00:00 EST 2021

Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer.

Frontiers in cell and developmental biology

You X,Li M,Cai H,Zhang W,Hong Y,Gao W,Liu Y,Liang X,Wu T,Chen F,Su D

Sat Oct 16 00:00:00 EDT 2021

Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer.

Frontiers in cell and developmental biology

You X,Li M,Cai H,Zhang W,Hong Y,Gao W,Liu Y,Liang X,Wu T,Chen F,Su D

Sat Oct 16 00:00:00 EDT 2021

Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.

Cell death & disease

Lei WL,Li YY,Meng TG,Ning Y,Sun SM,Zhang CH,Gui Y,Wang ZB,Qian WP,Sun QY

Mon Sep 27 00:00:00 EDT 2021

Responses of intestinal morphology and function in offspring to heat stress in primiparous sows during late gestation.

Journal of thermal biology

Guo H,He J,Yang X,Zheng W,Yao W

Wed Apr 01 00:00:00 EDT 2020

Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.

Acta pharmacologica Sinica

Fu C,Hao S,Xu X,Zhou J,Liu Z,Lu H,Wang L,Jin W,Li S

Wed May 01 00:00:00 EDT 2019

Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.

Acta pharmacologica Sinica

Fu C,Hao S,Xu X,Zhou J,Liu Z,Lu H,Wang L,Jin W,Li S

Wed May 01 00:00:00 EDT 2019

Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.

Acta pharmacologica Sinica

Fu C,Hao S,Xu X,Zhou J,Liu Z,Lu H,Wang L,Jin W,Li S

Wed May 01 00:00:00 EDT 2019

Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.

Acta pharmacologica Sinica

Fu C,Hao S,Xu X,Zhou J,Liu Z,Lu H,Wang L,Jin W,Li S

Wed May 01 00:00:00 EDT 2019

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

EMBO molecular medicine

Zhao M,Xiong X,Ren K,Xu B,Cheng M,Sahu C,Wu K,Nie Y,Huang Z,Blumberg RS,Han X,Ruan HB

Wed Aug 01 00:00:00 EDT 2018

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

EMBO molecular medicine

Zhao M,Xiong X,Ren K,Xu B,Cheng M,Sahu C,Wu K,Nie Y,Huang Z,Blumberg RS,Han X,Ruan HB

Wed Aug 01 00:00:00 EDT 2018

NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Yang F,Wang Z,Wei X,Han H,Meng X,Zhang Y,Shi W,Li F,Xin T,Pang Q,Yi F

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Friedreich ataxia region gene X104 (tight junction protein ZO-2); Tight junction protein 2; Tight junction protein ZO-2; Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2); zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2

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Gene Aliases: C9DUPq21.11; DFNA51; DUP9q21.11; PFIC4; TJP2; X104; ZO-2; ZO2

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UniProt ID: (Human) Q8N756, (Mouse) Q9Z0U1

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Entrez Gene ID: (Human) 9414, (Mouse) 21873, (Rat) 115769

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Function(s)

guanylate kinase activity protein binding protein C-terminus binding protein domain specific binding protein binding, bridging cadherin binding involved in cell-cell adhesion tight junction

Process(es)

hippo signaling GMP metabolic process GDP metabolic process intestinal absorption TNFSF11-mediated signaling pathway establishment of endothelial intestinal barrier regulation of membrane permeability cell-cell adhesion negative regulation of osteoclast development response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-2 Polyclonal Antibody

ZO-2 Antibody (18900-1-AP) in ICC/IF

Product Details

18900-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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