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ZRANB3 Antibody (PA5-97227) in WB
Western blot analysis of ZRANB3 in extracts of various cell lines. Samples were incubated with ZRANB3 Polyclonal antibody (Product # PA5-97227) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-97227
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 818-1077 of human ZRANB3 (NP_0012734971)
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809029
Product Specific Information
Immunogen sequence: ITKQQTKQNC TKRYITKEDV AVASMDKVKN VGGHVRLITK ESRPRDPFTK KLLEDGACVP FLNPYTVQAD LTVKPSTSKG YLQAVDNEGN PLCLRCQQPT CQTKQACKAN SWDSRFCSLK CQEEFWIRSN NSYLRAKVFE TEHGVCQLCN VNAQELFLRL RDAPKSQRKN LLYATWTSKL PLEQLNEMIR NPGEGHFWQV DHIKPVYGGG GQCSLDNLQT LCTVCHKERT ARQAKERSQV RRQSLASKHG SDITRFLVKK; Positive Samples: HeLa, Raji, Jurkat, 293T, Mouse liver, Mouse heart, Mouse brain, Rat liver; Cellular Location: Chromosome, Nucleus
Target Information
DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AH2; Annealing helicase 2; DNA annealing helicase and endonuclease ZRANB3; Zinc finger Ran-binding domain-containing protein 3; zinc finger, RAN-binding domain containing 3
Gene Aliases: 4933425L19Rik; AH2; AI316834; C730006D09; RGD1564952; ZRANB3
UniProt ID: (Human) Q5FWF4, (Mouse) Q6NZP1
Entrez Gene ID: (Human) 84083, (Rat) 304761, (Mouse) 226409
DNA helicase activity
endodeoxyribonuclease activity
protein binding
ATP binding
zinc ion binding
annealing helicase activity
K63-linked polyubiquitin binding
DNA binding
nucleotide binding
nucleic acid binding
catalytic activity
helicase activity
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
metal ion binding
DNA metabolism protein
DNA strand renaturation
DNA repair
cellular response to DNA damage stimulus
response to UV
replication fork processing
DNA rewinding
negative regulation of DNA recombination
replication fork protection
nucleic acid phosphodiester bond hydrolysis
DNA catabolic process, endonucleolytic
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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