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FIGURE: 1 / 1
ZSCAN4 Antibody (PA5-88295) in WB
Western blot analysis of ZSCAN4 in 293T-ZSCAN4 cells. Samples were incubated with ZSCAN4 Polyclonal antibody (Product # PA5-88295) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88295
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human ZSCAN4 (NP_6898901)
Conjugate
Form
Liquid
Concentration
0.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804807
Product Specific Information
Positive Samples: SH-SY5Y, Mouse brain; Cellular Location: Chromosome, Nucleus, telomere
Immunogen sequence: MALDLRTIFQ CEPSENNLGS ENSAFQQSQG PAVQREEGIS EFSRMVLNSF QDSNNSYARQ ELQRLYRIFH SWLQPEKHSK DEIISLLVLE QFMIGGHCND KASVKEKWKS SGKNLERFIE DLTDDSINPP ALVHVHMQGQ EALFSEDMPL RDVIVHLTKQ VNAQTTREAN MGTPSQTSQD TSLETGQGYE DEQDGWNSSS KTTRVNENIT NQGNQIVSLI IIQEENG
Target Information
Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Zinc finger and SCAN domain-containing protein 4; Zinc finger protein 494
Gene Aliases: ZNF494; ZSCAN4
UniProt ID: (Human) Q8NAM6
Entrez Gene ID: (Human) 201516
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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