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ZW10 Antibody (PA5-116151) in ICC/IF
Immunocytochemistry analysis of ZW10 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116151) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZW10(Accession O43264), corresponding to amino acid residues K449-T499.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900785
Product Specific Information
Antibody detects endogenous levels of total ZW10.
Target Information
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Centromere/kinetochore protein zw10 homolog; H KNTC1AP; MGC149821; zeste white 10 homolog; ZW10 homolog, centromere/kinetochore protein; ZW10, kinetochore associated, homolog
Gene Aliases: 6330566F14Rik; HZW10; KNTC1AP; MmZw10; ZW10
UniProt ID: (Human) O43264, (Mouse) O54692, (Rat) Q4V8C2
Entrez Gene ID: (Human) 9183, (Mouse) 26951, (Rat) 363059
mitotic sister chromatid segregation
establishment of mitotic spindle orientation
protein complex assembly
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
Golgi organization
sister chromatid cohesion
mitotic metaphase plate congression
mitotic cell cycle checkpoint
mitotic spindle assembly checkpoint
regulation of exit from mitosis
meiotic nuclear division
protein transport
protein localization to kinetochore
cell division
transport
cell cycle
mitotic nuclear division
vesicle-mediated transport
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