Invitrogen

Zap-70 Monoclonal Antibody (1E7.2)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

33 References

View all (97) Zap-70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Zap-70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Zap-70 Antibody (MA1-21350) in ICC/IF

Immunofluorescence analysis of Zap-70 was performed using 70 confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Zap-70 Monoclonal Antibody (1E7.2) (Product # MA1-21350) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More

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Product Details

MA1-21350

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 30 publications 30 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E7.2

Immunogen

GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.7 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_562001

Product Specific Information

Positive control of Jurkat cell lysate suggested.

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia.

Hematology (Amsterdam, Netherlands)

Vroblova V,Smolej L,Krejsek J

MA1-21350 was used in flow cytometry to discuss using flow cytometry to assess Zap-70 in chronic lymphocytic leukemia.

Sat Sep 01 00:00:00 EDT 2012

Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia.

Immunology letters

Zucchetto A,Cattarossi I,Nanni P,Zaina E,Prato G,Gilestro M,Marconi D,Bulian P,Rossi FM,Del Vecchio L,Omedè P,Geuna M,Del Poeta G,Gattei V

MA1-21350 was used in flow cytometry to identify the immunophenotypic signature of chronic lymphocytic leukemia subsets with different prognosis

Sun Jan 30 00:00:00 EST 2011

High expression of the immature laminin receptor protein correlates with mutated IGVH status and predicts a favorable prognosis in chronic lymphocytic leukemia.

Leukemia research

Friedrichs B,Siegel S,Reimer R,Barsoum A,Coggin J,Kabelitz D,Heidorn K,Schulte C,Schmitz N,Zeis M

MA1-21350 was used in flow cytometry to assess immature laminin receptor expression on patients with B cell malignancies

Wed Jun 01 00:00:00 EDT 2011

Methodological comparison of two anti-ZAP-70 antibodies.

Cytometry. Part B, Clinical cytometry

Degheidy HA,Venzon DJ,Farooqui MZ,Abbasi F,Arthur DC,Wilson WH,Wiestner A,Stetler-Stevenson MA,Marti GE

MA1-21350 was used in flow cytometry to discuss variance in the detection of ZAP-70 expression by clinical laboratories.

Thu Sep 01 00:00:00 EDT 2011

Prospective study of clinical and biological prognostic factors at diagnosis in patients with early stage B-cell chronic lymphocytic leukemia.

Leukemia & lymphoma

Oliveira AC,de la Banda E,Domingo-Domenech E,Encuentra M,Mercadal S,Domingo A,Alonso E,Espinet B,Grau J,De Sevilla AF,Gonzalez-Barca E

MA1-21350 was used in flow cytometry to identify prognostic factors for chronic lymphocytic leukemia using a retrospective series.

Tue Mar 01 00:00:00 EST 2011

Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.

PloS one

Deswal S,Schulze AK,Höfer T,Schamel WW

MA1-21350 was used in flow cytometry to discuss how to study activation-dependent phosphorylation of proteins using mathematical modeling

Tue Dec 06 00:00:00 EST 2011

Recruitment of PKC-betaII to lipid rafts mediates apoptosis-resistance in chronic lymphocytic leukemia expressing ZAP-70.

Leukemia

zum Büschenfelde CM,Wagner M,Lutzny G,Oelsner M,Feuerstacke Y,Decker T,Bogner C,Peschel C,Ringshausen I

MA1-21350 was used in immunocytochemistry to investigate the contribution of ZAP-70 to chronic lymphocytic leukemia aggressiveness

Fri Jan 01 00:00:00 EST 2010

Significant change in ZAP-70 expression during the course of chronic lymphocytic leukemia.

European journal of haematology

Vroblova V,Vrbacky F,Hrudkova M,Jankovicova K,Schmitzova D,Maly J,Krejsek J,Smolej L

MA1-21350 was used in flow cytometry to examine expression of ZAP-70 throughout the disease course of chronic lymphocytic leukemia

Tue Jun 01 00:00:00 EDT 2010

Comprehensive biomarker and genomic analysis identifies p53 status as the major determinant of response to MDM2 inhibitors in chronic lymphocytic leukemia.

Blood

Saddler C,Ouillette P,Kujawski L,Shangary S,Talpaz M,Kaminski M,Erba H,Shedden K,Wang S,Malek SN

MA1-21350 was used in flow cytometry to investigate chronic lymphocytic leukemia patient-derived samples' responses to MDM2 inhibitors and correlate responses with clinically important biomarkers

Fri Feb 01 00:00:00 EST 2008

FCRL2 expression predicts IGHV mutation status and clinical progression in chronic lymphocytic leukemia.

Blood

Li FJ,Ding S,Pan J,Shakhmatov MA,Kashentseva E,Wu J,Li Y,Soong SJ,Chiorazzi N,Davis RS

MA1-21350 was used in flow cytometry to assess the prognostic potential of Fc receptor-like molecules for chronic lymphocytic leukemia

Tue Jul 01 00:00:00 EDT 2008

Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method.

Haematologica

Gachard N,Salviat A,Boutet C,Arnoulet C,Durrieu F,Lenormand B,Leprêtre S,Olschwang S,Jardin F,Lafage-Pochitaloff M,Penther D,Sainty D,Reminieras L,Feuillard J,Béné MC

MA1-21350 was used in flow cytometry to determine ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia

Fri Feb 01 00:00:00 EST 2008

Humoral immune responses against the immature laminin receptor protein show prognostic significance in patients with chronic lymphocytic leukemia.

Journal of immunology (Baltimore, Md. : 1950)

Friedrichs B,Siegel S,Kloess M,Barsoum A,Coggin J,Rohrer J,Jakob I,Tiemann M,Heidorn K,Schulte C,Kabelitz D,Steinmann J,Schmitz N,Zeis M

MA1-21350 was used in flow cytometry to test if the humoral immunity specific for the chronic lymphocytic leukemia-associated Ag oncofetal Ag/immature laminin receptor has a prognostic value in chronic lymphocytic leukemia

Thu May 01 00:00:00 EDT 2008

An improved flow cytometric method using FACS Lysing Solution for measurement of ZAP-70 expression in B-cell chronic lymphocytic leukemia.

Cytometry. Part B, Clinical cytometry

Bekkema R,Tadema A,Daenen SM,Kluin-Nelemans HC,Mulder AB

MA1-21350 was used in flow cytometry to describe an improved fluorescence-activate cell sorting lysing solution to measure ZAP-70 expression in B-cell chronic lymphocytic leukemia

Tue Jan 01 00:00:00 EST 2008

Optimization of flow cytometric measurement of ZAP-70 in chronic lymphocytic leukemia.

Cytometry. Part B, Clinical cytometry

Preobrazhensky SN,Bahler DW

MA1-21350 was used in flow cytometry to develop an optimized cell staining procedure for detection of zeta-chain associated protein-70 via flow cytometry

Sat Mar 01 00:00:00 EST 2008

ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.

Leukemia

Zanotti R,Ambrosetti A,Lestani M,Ghia P,Pattaro C,Remo A,Zanetti F,Stella S,Perbellini O,Prato G,Guida G,Caligaris-Cappio F,Menestrina F,Pizzolo G,Chilosi M

MA1-21350 was used in flow cytometry to discuss the use of ZAP-70 expression to identify patients with poor prognosis of chronic lymphocytic leukemia

Mon Jan 01 00:00:00 EST 2007

Familial B-cell chronic lymphocytic leukemia: analysis of cytogenetic abnormalities, immunophenotypic profiles, and immunoglobulin heavy chain gene usage.

American journal of clinical pathology

Aoun P,Zhou G,Chan WC,Page C,Neth K,Pickering D,Sanger W,Quinn-Laquer B,Watson P,Lynch JF,Lynch HT,Weisenburger DD

MA1-21350 was used in flow cytometry to study the cytogenetic, immunophenotypic, and VH gene usage characteristics of a family with B-cell chronic lymphocytic leukemia

Mon Jan 01 00:00:00 EST 2007

Modern prognostic factors and angiogenesis in chronic lymphocytic leukemia: more data needed.

Leukemia research

Smolej L,Andrys C,Vroblova V

MA1-21350 was used in flow cytometry to discuss prognostic factors ZAP-70, vascular endothelial growth factor and basic fibroblast growth factor and angiogenesis in chronic lymphocytic leukemia

Sat Dec 01 00:00:00 EST 2007

National standardization of ZAP-70 determination by flow cytometry: the French experience.

Cytometry. Part B, Clinical cytometry

Le Garff-Tavernier M,Ticchioni M,Brissard M,Salmon C,Raynaud S,Davi F,Bernard A,Merle-Béral H,Ajchenbaum-Cymbalista F,Letestu R

MA1-21350 was used in flow cytometry to report a simple, fast, and reproducible flow cytometry method to detect ZAP-70 expression

Thu Mar 01 00:00:00 EST 2007

Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells.

Blood

de Totero D,Meazza R,Zupo S,Cutrona G,Matis S,Colombo M,Balleari E,Pierri I,Fabbi M,Capaia M,Azzarone B,Gobbi M,Ferrarini M,Ferrini S

MA1-21350 was used in flow cytometry investigate IL-21 signaling in normal and chronic lymphocytic leukemia B cells

Mon May 01 00:00:00 EDT 2006

ZAP-70 is expressed by normal and malignant human B-cell subsets of different maturational stage.

Leukemia

Scielzo C,Camporeale A,Geuna M,Alessio M,Poggi A,Zocchi MR,Chilosi M,Caligaris-Cappio F,Ghia P

MA1-21350 was used in flow cytometry to investigate ZAP-70 expression in B-cell malignancies

Sat Apr 01 00:00:00 EST 2006

The effect of immunoglobulin VH gene mutation status and other prognostic factors on the incidence of major infections in patients with chronic lymphocytic leukemia.

Cancer

Francis S,Karanth M,Pratt G,Starczynski J,Hooper L,Fegan C,Pepper C,Valcarcel D,Milligan DW,Delgado J

MA1-21350 was used in flow cytometry to discuss the features of patients with chronic lymphocytic leukemia and infections

Fri Sep 01 00:00:00 EDT 2006

A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.

Journal of cellular physiology

Zucchetto A,Bomben R,Dal Bo M,Sonego P,Nanni P,Rupolo M,Bulian P,Dal Maso L,Del Poeta G,Del Principe MI,Degan M,Gattei V

MA1-21350 was used in flow cytometry describe an immunophenotypic scoring system for B-cell chronic lymphocytic leukemia

Mon May 01 00:00:00 EDT 2006

Feasibility of an easily applicable method of ZAP-70 measurement in chronic lymphocytic leukemia in the routine flow cytometry setting: A methodological approach.

Annals of hematology

Passam F,Tachynopoulou V,Skoumi D,Tsompanakou A,Stavropoulos-Giokas A,Vadikolia C,Anagnostopoulos A,Paterakis G

MA1-21350 was used in flow cytometry to use flow cytometry to assess the use of ZAP-70 expression as marker for B chronic lymphocytic leukemia

Wed Nov 01 00:00:00 EST 2006

A robust ratio metric method for analysis of Zap-70 expression in chronic lymphocytic leukemia (CLL).

Cytometry. Part B, Clinical cytometry

Bakke AC,Purtzer Z,Leis J,Huang J

MA1-21350 was used in flow cytometry to examine Zap-70 expression in patients with chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL).

Cytometry. Part B, Clinical cytometry

Bojarska-Junak A,Giannopoulos K,Kowal M,Dmoszyńska A,Roliński J

MA1-21350 was used in flow cytometry to investigate novel monoclonal antibodies to ZAP-70 to detect B-cell chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

Comparison of bone marrow and peripheral blood ZAP-70 status examined by flow cytometric immunophenotyping in patients with chronic lymphocytic leukemia.

Cytometry. Part B, Clinical cytometry

Sheridan R,Mounajjed T,Ehrmann DE,Hurtubise PE,Schrager JA

MA1-21350 was used in flow cytometry to compare bone marrow samples with peripheral blood for ZAP-70 expression in patients with chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

ZAP-70 expression in B-cell chronic lymphocytic leukemia: evaluation by external (isotypic) or internal (T/NK cells) controls and correlation with IgV(H) mutations.

Cytometry. Part B, Clinical cytometry

Zucchetto A,Bomben R,Bo MD,Nanni P,Bulian P,Rossi FM,Del Principe MI,Santini S,Del Poeta G,Degan M,Gattei V

MA1-21350 was used in flow cytometry to discuss the prognostic relevance and predictive power of ZAP-70 expression for patients with chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

Clinical utility of assessing ZAP-70 and CD38 in chronic lymphocytic leukemia.

Cytometry. Part B, Clinical cytometry

Rassenti LZ,Kipps TJ

MA1-21350 was used in flow cytometry to discuss the clinical utility of assessing ZAP-70 and CD38 in chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

ZAP-70 in CLL: towards standardization of a biomarker for patient management: history of clinical cytometry special issue.

Cytometry. Part B, Clinical cytometry

Marti G,Orfao A,Goolsby C

MA1-21350 was used in flow cytometry to discuss the use of ZAP-70 in chronic lymphocytic leukemia as a biomarker for patient management

Sat Jul 15 00:00:00 EDT 2006

ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.

Cytometry. Part B, Clinical cytometry

Best OG,Ibbotson RE,Parker AE,Davis ZA,Orchard JA,Oscier DG

MA1-21350 was used in flow cytometry to discuss the use of ZAP-70 expression to examine chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process.

Cytometry. Part B, Clinical cytometry

Letestu R,Rawstron A,Ghia P,Villamor N,Boeckx N,Boettcher S,Buhl AM,Duerig J,Ibbotson R,Kroeber A,Langerak A,Le Garff-Tavernier M,Mockridge I,Morilla A,Padmore R,Rassenti L,Ritgen M,Shehata M,Smolewski P,Staib P,Ticchioni M,Walker C,Ajchenbaum-Cymbalista F

MA1-21350 was used in flow cytometry to review factors that influences the detection of ZAP-70 by flow cytometry

Sat Jul 15 00:00:00 EDT 2006

An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia.

Cytometry. Part B, Clinical cytometry

Shankey TV,Forman M,Scibelli P,Cobb J,Smith CM,Mills R,Holdaway K,Bernal-Hoyos E,Van Der Heiden M,Popma J,Keeney M

MA1-21350 was used in flow cytometry to review the use of ZAP-70 expression as a biomarker in patients with chronic lymphocytic leukemia

Sat Jul 15 00:00:00 EDT 2006

Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory.

Clinical and laboratory haematology

Gibbs G,Bromidge T,Howe D,Hopkins J,Johnson S

MA1-21350 was used in flow cytometry to measure ZAP-70 in cases of chronic lymphocytic leukemia

Mon Aug 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; syk-related protein tyrosine kinase; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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Gene Aliases: ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70

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UniProt ID: (Human) P43403, (Mouse) P43404

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Entrez Gene ID: (Human) 7535, (Mouse) 22637, (Rat) 301348

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Function(s)

phosphotyrosine binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups molecular_function

Process(es)

adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response protein phosphorylation inflammatory response immune response transmembrane receptor protein tyrosine kinase signaling pathway peptidyl-tyrosine phosphorylation T cell differentiation positive regulation of cell adhesion mediated by integrin intracellular signal transduction peptidyl-tyrosine autophosphorylation T cell activation B cell activation positive regulation of mast cell degranulation beta selection positive thymic T cell selection negative thymic T cell selection innate immune response positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway T cell aggregation T cell migration regulation of platelet aggregation immune system process phosphorylation cell migration cell differentiation thymic T cell selection protein autophosphorylation biological_process

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Zap-70 Monoclonal Antibody (1E7.2)

Zap-70 Antibody (MA1-21350) in ICC/IF

Product Details

MA1-21350

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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