Invitrogen

alpha Catenin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

23 References

View all (11) alpha Catenin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

alpha Catenin Antibody in Western Blot (WB)

FIGURE: 1 / 15

alpha Catenin Antibody (71-1200) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A431 (Lane 1), A549 (Lane 2), CaCo2 (Lane 3), MCF7 (lane 4) and HT-29 (lane 5). The blots were probed with Anti-alpha Catenin Rabbit Polyclonal Antibody (Product # 71-1200, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two bands ~ 100 and 59 kDa corresponding to alpha Catenin was observed across cell lines tested. Known quantity of... View More

alpha Catenin Antibody in Immunoprecipitation (IP)

alpha Catenin Antibody in Immunocytochemistry (ICC/IF)

alpha Catenin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

alpha Catenin Antibody in Immunohistochemistry (IHC)

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Product Details

71-1200

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 9 publications 9 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat, Xenopus

Published species

Bovine, Dog, Hamster, Human, Mouse, Rat, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human alpha Catenin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533974

Target Information

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial barrier dysfunction in systemic inflammation is mediated by soluble VE-cadherin interfering VE-PTP signaling.

iScience

Knop JL,Burkard N,Danesh M,Kintrup S,Dandekar T,Srivastava M,Springer R,Hiermaier M,Wagner NM,Waschke J,Flemming S,Schlegel N

71-1200 was used in Western Blot to demonstrate evidence for a causative role of sVE-cadherin to induce loss of endothelial barrier function.

Fri Oct 20 00:00:00 EDT 2023

Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor.

Development (Cambridge, England)

Otsu K,Ida-Yonemochi H,Ikezaki S,Ema M,Hitomi J,Ohshima H,Harada H

71-1200 was used in Immunohistochemistry to study the role of molecular oxygen in regulating the proliferation of epithelial stem cells.

Mon Feb 15 00:00:00 EST 2021

Mechanochemical control of epidermal stem cell divisions by B-plexins.

Nature communications

Jiang C,Javed A,Kaiser L,Nava MM,Xu R,Brandt DT,Zhao D,Mayer B,Fernández-Baldovinos J,Zhou L,Höß C,Sawmynaden K,Oleksy A,Matthews D,Weinstein LS,Hahn H,Gröne HJ,Graumann PL,Niessen CM,Offermanns S,Wickström SA,Worzfeld T

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunocytochemistry

Fri Feb 26 00:00:00 EST 2021

Mechanochemical control of epidermal stem cell divisions by B-plexins.

Nature communications

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunocytochemistry

Fri Feb 26 00:00:00 EST 2021

Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion.

Journal of cell science

Wee K,Hediyeh-Zadeh S,Duszyc K,Verma S,N Nanavati B,Khare S,Varma A,Daly RJ,Yap AS,Davis MJ,Budnar S

71-1200 was used in Immunocytochemistry-immunoflourescence to analyse the ability of Snail to promote the morphogenetic phenomenon of epithelial cell extrusion.

Mon Jul 06 00:00:00 EDT 2020

Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion.

Molecular biology of the cell

Teo JL,Tomatis VM,Coburn L,Lagendijk AK,Schouwenaar IM,Budnar S,Hall TE,Verma S,McLachlan RW,Hogan BM,Parton RG,Yap AS,Gomez GA

71-1200 was used in Immunocytochemistry to report a complementary process of cytoskeletal relaxation that occurs when cortical contractility is down-regulated at the junctions between those neighbor cells themselves.

Sun Nov 01 00:00:00 EDT 2020

Shigella flexneri Disruption of Cellular Tension Promotes Intercellular Spread.

Cell reports

Duncan-Lowey JK,Wiscovitch AL,Wood TE,Goldberg MB,Russo BC

71-1200 was used in Western Blotting to investigate the formation of plasma membrane protrusions and intercellular spread bin Shigella flexneri.

Tue Nov 24 00:00:00 EST 2020

Knockout of SORBS2 Protein Disrupts the Structural Integrity of Intercalated Disc and Manifests Features of Arrhythmogenic Cardiomyopathy.

Journal of the American Heart Association

Ding Y,Yang J,Chen P,Lu T,Jiao K,Tester DJ,Giudicessi JR,Jiang K,Ackerman MJ,Li Y,Wang DW,Lee HC,Wang DW,Xu X

71-1200 was used in Western Blotting to conduct a detailed expression and subcellular localization analysis of Sorbs2 ortholog in mice and a phenotypic characterization in Sorbs2 knockout mice.

Tue Sep 01 00:00:00 EDT 2020

Telophase correction refines division orientation in stratified epithelia.

eLife

Lough KJ,Byrd KM,Descovich CP,Spitzer DC,Bergman AJ,Beaudoin GM,Reichardt LF,Williams SE

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunofluorescence

Fri Dec 13 00:00:00 EST 2019

Telophase correction refines division orientation in stratified epithelia.

eLife

Lough KJ,Byrd KM,Descovich CP,Spitzer DC,Bergman AJ,Beaudoin GM,Reichardt LF,Williams SE

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunofluorescence

Fri Dec 13 00:00:00 EST 2019

α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart.

Development (Cambridge, England)

Vite A,Zhang C,Yi R,Emms S,Radice GL

71-1200 was used in Immunohistochemistry-immunofluorescence to investigate the relationship between intercalated disc maturation and proliferation.

Thu Mar 08 00:00:00 EST 2018

Tyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1.

Nature communications

Liang X,Budnar S,Gupta S,Verma S,Han SP,Hill MM,Daly RJ,Parton RG,Hamilton NA,Gomez GA,Yap AS

71-1200 was used in Immunocytochemistry-immunoflourescence to identify a novel function of cortactin as a regulator of RhoA signaling.

Thu Oct 05 00:00:00 EDT 2017

Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion.

Journal of cell science

Van Itallie CM,Tietgens AJ,Aponte A,Fredriksson K,Fanning AS,Gucek M,Anderson JM

71-1200 was used in Immunocytochemistry to express E-cadherin fused to biotin ligase in MDCK epithelial cells, and identify neighbouring proteins that are biotinylated.

Sat Feb 15 00:00:00 EST 2014

Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion.

Journal of cell science

Van Itallie CM,Tietgens AJ,Aponte A,Fredriksson K,Fanning AS,Gucek M,Anderson JM

71-1200 was used in Immunocytochemistry to express E-cadherin fused to biotin ligase in MDCK epithelial cells, and identify neighbouring proteins that are biotinylated.

Sat Feb 15 00:00:00 EST 2014

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

71-1200 was used in immunocytochemistry to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 EST 2013

Adherens junction proteins in the hamster uterus: their contributions to the success of implantation.

Biology of reproduction

Luan L,Ding T,Stinnett A,Reese J,Paria BC

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunofluorescence

Tue Nov 01 00:00:00 EDT 2011

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

N-cadherin haploinsufficiency affects cardiac gap junctions and arrhythmic susceptibility.

Journal of molecular and cellular cardiology

Li J,Levin MD,Xiong Y,Petrenko N,Patel VV,Radice GL

71-1200 was used in immunocytochemistry to explore the relationship between N-cadherin and connexin43 in the heart

Sat Mar 01 00:00:00 EST 2008

E-cadherin suppression accelerates squamous cell carcinoma progression in three-dimensional, human tissue constructs.

Cancer research

Margulis A,Zhang W,Alt-Holland A,Crawford HC,Fusenig NE,Garlick JA

Tue Mar 01 00:00:00 EST 2005

E-cadherin suppression accelerates squamous cell carcinoma progression in three-dimensional, human tissue constructs.

Cancer research

Margulis A,Zhang W,Alt-Holland A,Crawford HC,Fusenig NE,Garlick JA

Tue Mar 01 00:00:00 EST 2005

Proliferation, cell cycle exit, and onset of terminal differentiation in cultured keratinocytes: pre-programmed pathways in control of C-Myc and Notch1 prevail over extracellular calcium signals.

The Journal of investigative dermatology

Kolly C,Suter MM,Müller EJ

Sun May 01 00:00:00 EDT 2005

Proliferation, cell cycle exit, and onset of terminal differentiation in cultured keratinocytes: pre-programmed pathways in control of C-Myc and Notch1 prevail over extracellular calcium signals.

The Journal of investigative dermatology

Kolly C,Suter MM,Müller EJ

Sun May 01 00:00:00 EDT 2005

EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy.

Cancer research

Osta WA,Chen Y,Mikhitarian K,Mitas M,Salem M,Hannun YA,Cole DJ,Gillanders WE

Sun Aug 15 00:00:00 EDT 2004

EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy.

Cancer research

Osta WA,Chen Y,Mikhitarian K,Mitas M,Salem M,Hannun YA,Cole DJ,Gillanders WE

Sun Aug 15 00:00:00 EDT 2004

Vinculin, VASP, and profilin are coordinately regulated during actin remodeling in epithelial cells, which requires de novo protein synthesis and protein kinase signal transduction pathways.

Journal of cellular physiology

Quinlan MP

Published figure using alpha Catenin polyclonal antibody (Product # 71-1200) in Immunofluorescence

Sun Aug 01 00:00:00 EDT 2004

Myofibroblast development is characterized by specific cell-cell adherens junctions.

Molecular biology of the cell

Hinz B,Pittet P,Smith-Clerc J,Chaponnier C,Meister JJ

Wed Sep 01 00:00:00 EDT 2004

ROCK mediates thrombin's endothelial barrier dysfunction.

American journal of physiology. Cell physiology

Carbajal JM,Gratrix ML,Yu CH,Schaeffer RC

71-1200 was used in immunocytochemistry to test if ROCK has a role in thrombin-induced endothelial monolayer hyperpermeability.

Sat Jul 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; Alpha N-catenin; alpha(E)-catenin; alpha(N)-catenin; alpha-catenin; Alpha-catenin-related protein; alpha-E-catenin; alpha-N-catenin; alphaE-catenin; alphaN-catenin; cadherin associated protein; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; CAP102; catenin; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; catenin alpha 1; catenin alpha 2; Catenin alpha-1; Catenin alpha-2; Catenin alpha1; Catenin alpha2; catenin, alpha 1; Catenin-alpha1; Catenin-alpha2; cerebellar deficient folia; FLJ36832; FLJ52416; HGNC:2509; Neural alpha-catenin; NY-REN-13 antigen; Renal carcinoma antigen NY-REN-13

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Gene Aliases: 2010010M04Rik; AA517462; AI481747; AI988031; CAP-R; CAP102; CAPR; Catna; Catna1; Catna2; cdf; chp; CT114; CTNNA1; CTNNA2; CTNR; MDPT2

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UniProt ID: (Human) P35221, (Human) P26232, (Chicken) P30997, (Mouse) P26231, (Mouse) Q61301

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Entrez Gene ID: (Human) 1495, (Chicken) 416184, (Human) 1496, (Chicken) 396035, (Rat) 307505, (Mouse) 12385, (Mouse) 12386, (Rat) 297357

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Function(s)

structural molecule activity protein binding beta-catenin binding vinculin binding poly(A) RNA binding gamma-catenin binding cadherin binding actin filament binding cadherin binding involved in cell-cell adhesion structural constituent of cytoskeleton non-motor actin binding protein

Process(es)

ovarian follicle development actin filament organization cell adhesion establishment or maintenance of cell polarity negative regulation of neuroblast proliferation aging male gonad development gap junction assembly axon regeneration adherens junction organization cellular protein localization odontogenesis of dentin-containing tooth apical junction assembly response to estrogen positive regulation of smoothened signaling pathway positive regulation of muscle cell differentiation protein heterooligomerization cellular response to indole-3-methanol epithelial cell-cell adhesion negative regulation of cell motility negative regulation of integrin-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of extrinsic apoptotic signaling pathway in absence of ligand cytoskeleton organization axonogenesis single organismal cell-cell adhesion radial glia guided migration of Purkinje cell dendrite morphogenesis brain morphogenesis regulation of synapse structural plasticity prepulse inhibition neural crest cell migration protein localization epithelial cell differentiation trigeminal ganglion formation cell-cell adhesion response to organic cyclic compound regulation of cell proliferation negative regulation of apoptotic process multicellular organism development cell differentiation

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alpha Catenin Polyclonal Antibody

alpha Catenin Antibody (71-1200) in WB

Product Details

71-1200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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