Invitrogen

alpha Catenin Monoclonal Antibody (1G5)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (11) alpha Catenin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

alpha Catenin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

alpha Catenin Antibody (MA1-2000) in ICC/IF

Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Pro... View More

alpha Catenin Antibody in Western Blot (WB)

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Product Details

MA1-2000

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G5

Immunogen

90 kDa MBP/alpha catenin fusion protein purified on amylose column.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326077

Product Specific Information

MA1-2000 detects alpha catenin from human samples.

MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.

Target Information

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exclusion from spheroid formation identifies loss of essential cell-cell adhesion molecules in colon cancer cells.

Scientific reports

Stadler M,Scherzer M,Walter S,Holzner S,Pudelko K,Riedl A,Unger C,Kramer N,Weil B,Neesen J,Hengstschläger M,Dolznig H

MA1-2000 was used in Western Blotting to identify molecules responsible for subpopulations of non-spheroid forming (NSF) colon cancer cell lines with distinct phenotypes during in vitro spheroid formation.

Thu Jan 18 00:00:00 EST 2018

Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

PLoS neglected tropical diseases

Sato H,Coburn J

MA1-2000 was used in Immunocytochemistry-immunoflourescence to elucidate the pathogenic mechanisms of Leptospira interrogans infection.

Sat Jul 01 00:00:00 EDT 2017

Integration of left-right Pitx2 transcription and Wnt signaling drives asymmetric gut morphogenesis via Daam2.

Developmental cell

Welsh IC,Thomsen M,Gludish DW,Alfonso-Parra C,Bai Y,Martin JF,Kurpios NA

MA1-2000 was used in immunoprecipitation to study the role of Daam2 in the mechanism by which Pitx2 and Wnt participate in asymmetric gut morphogenesis

Mon Sep 30 00:00:00 EDT 2013

Characterization of cadherin-24, a novel alternatively spliced type II cadherin.

The Journal of biological chemistry

Katafiasz BJ,Nieman MT,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the functional and structural properties of cadherin 24 and its alternative splicing variant

Fri Jul 25 00:00:00 EDT 2003

Mechanism of extracellular domain-deleted dominant negative cadherins.

Journal of cell science

Nieman MT,Kim JB,Johnson KR,Wheelock MJ

MA1-2000 was used in western blot to investigate the functions of extracellular domain-deleted dominant-negative cadherin in cells

Sat May 01 00:00:00 EDT 1999

Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.

The Journal of cell biology

Islam S,Carey TE,Wolf GT,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the importance of N-cadherin in cell-cell adhesion

Sun Dec 01 00:00:00 EST 1996

Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.

The Journal of biological chemistry

Sacco PA,McGranahan TM,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin

Fri Aug 25 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: Alpha E-catenin; Alpha N-catenin; alpha-catenin; Alpha-catenin-related protein; alpha-E-catenin; alpha-N-catenin; alphaE-catenin; alphaN-catenin; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; catenin; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; Catenin alpha-1; Catenin alpha-2; Catenin alpha1; Catenin alpha2; Catenin-alpha1; Catenin-alpha2; FLJ36832; FLJ52416; HGNC:2509; NY-REN-13 antigen; Renal carcinoma antigen NY-REN-13

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Gene Aliases: CAP-R; CAP102; CAPR; CT114; CTNNA1; CTNNA2; CTNR; MDPT2

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UniProt ID: (Human) P35221, (Human) P26232

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Entrez Gene ID: (Human) 1495, (Human) 1496

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Function(s)

structural molecule activity protein binding beta-catenin binding vinculin binding poly(A) RNA binding gamma-catenin binding cadherin binding actin filament binding cadherin binding involved in cell-cell adhesion structural constituent of cytoskeleton non-motor actin binding protein

Process(es)

ovarian follicle development actin filament organization cell adhesion establishment or maintenance of cell polarity negative regulation of neuroblast proliferation aging male gonad development gap junction assembly axon regeneration adherens junction organization cellular protein localization odontogenesis of dentin-containing tooth apical junction assembly response to estrogen positive regulation of smoothened signaling pathway positive regulation of muscle cell differentiation protein heterooligomerization cellular response to indole-3-methanol epithelial cell-cell adhesion negative regulation of cell motility negative regulation of integrin-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of extrinsic apoptotic signaling pathway in absence of ligand cytoskeleton organization axonogenesis single organismal cell-cell adhesion radial glia guided migration of Purkinje cell dendrite morphogenesis brain morphogenesis regulation of synapse structural plasticity prepulse inhibition

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Catenin Monoclonal Antibody (1G5)

alpha Catenin Antibody (MA1-2000) in ICC/IF

Product Details

MA1-2000

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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