Invitrogen

alpha Catenin Monoclonal Antibody (alpha-CAT-7A4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

39 References

View all (11) alpha Catenin antibodies

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alpha Catenin Antibody (13-9700)

IP-MS enrichment of CTNNA1 (LFQ intensity): CTNNA1 was enriched 118-fold from HEPG2 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CTNNA1 antibody (Product # 13-9700). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of an... View More

alpha Catenin Antibody in Immunocytochemistry (ICC/IF)

alpha Catenin Antibody in Western Blot (WB)

alpha Catenin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

alpha Catenin Antibody in Immunohistochemistry (IHC)

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Product Details

13-9700

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 8 publications 8 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:250

View 14 publications 14 publications

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat, Xenopus

Published species

Bovine, Dog, Human, Mouse, Rat, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

alpha-CAT-7A4

Immunogen

Synthetic peptide derived from the C-terminus of mouse alpha-catenin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533044

Target Information

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vinculin strengthens the endothelial barrier during vascular development.

Vascular biology (Bristol, England)

van der Stoel MM,Kotini MP,Schoon RM,Affolter M,Belting HG,Huveneers S

13-9700 was used in Western Blotting to demonstrate that Vinculin strengthens the endothelial barrier and prevents vascular leakage in developing vessels.

Sun Jan 01 00:00:00 EST 2023

Loss of NECTIN1 triggers melanoma dissemination upon local IGF1 depletion.

Nature genetics

Ablain J,Al Mahi A,Rothschild H,Prasad M,Aires S,Yang S,Dokukin ME,Xu S,Dang M,Sokolov I,Lian CG,Zon LI

13-9700 was used in Immunocytochemistry-immunofluorescence to establish NECTIN1 as a major determinant of melanoma dissemination and uncovers a genetic control of the response to microenvironmental signals.

Thu Dec 01 00:00:00 EST 2022

Asymmetric distribution of dynamin-2 and β-catenin relative to tight junction spikes in alveolar epithelial cells.

Tissue barriers

Lynn KS,Easley KF,Martinez FJ,Reed RC,Schlingmann B,Koval M

13-9700 was used in Immunocytochemistry-immunofluorescence to suggest a novel role for dynamin-2 in tight junction spike formation by reorienting junction-associated actin.

Sat Jul 03 00:00:00 EDT 2021

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Development (Cambridge, England)

Guo D,Ru J,Mao F,Ouyang H,Ju R,Wu K,Liu Y,Liu C

13-9700 was used in Immunohistochemistry to highlight a crucial role of Prickle1-mediated polarized basement membrane secretion and deposition in PTD elongation.

Mon Nov 30 00:00:00 EST 2020

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

13-9700 was used in Immunocytochemistry-immunoflourescence to investigate proteins specifically expressed by restrictive endoneurial microvascular endothelium in four adult sural nerves.

Sat Jun 01 00:00:00 EDT 2019

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

13-9700 was used in Immunocytochemistry-immunoflourescence to investigate proteins specifically expressed by restrictive endoneurial microvascular endothelium in four adult sural nerves.

Sat Jun 01 00:00:00 EDT 2019

The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system.

Cell and tissue research

Domke LM,Franke WW

13-9700 was used in Immunocytochemistry-immunoflourescence to show the LSMCs of the peritubular wall monolayers are SMCs sensu stricto which are laterally connected by a novel architectonic system of arrays of vertical AJs located in overlapping cell protrusions.

Fri Feb 01 00:00:00 EST 2019

Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology.

Biosensors

Anchan A,Kalogirou-Baldwin P,Johnson R,Kho DT,Joseph W,Hucklesby J,Finlay GJ,O'Carroll SJ,Angel CE,Graham ES

13-9700 was used in Immunocytochemistry to study the kinetics of human melanoma cell invasion across human brain endothelium.

Mon Apr 15 00:00:00 EDT 2019

α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart.

Development (Cambridge, England)

Vite A,Zhang C,Yi R,Emms S,Radice GL

13-9700 was used in Western Blotting to investigate the relationship between intercalated disc maturation and proliferation.

Thu Mar 08 00:00:00 EST 2018

The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier.

Biosensors

Robilliard LD,Kho DT,Johnson RH,Anchan A,O'Carroll SJ,Graham ES

13-9700 was used in Immunocytochemistry to demonstrate the application of electrical cell-substrate impedance sensing technology for measuring differences in the formation of a strong and durable endothelial barrier model.

Wed Jul 04 00:00:00 EDT 2018

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Tissue barriers

Chen H,Lu R,Zhang YG,Sun J

13-9700 was used in Western Blotting to study the role of the vitamin D receptor in lung barrier integrity.

Mon Apr 01 00:00:00 EDT 2019

GDNF enhances human blood-nerve barrier function in vitro via MAPK signaling pathways.

Tissue barriers

Dong C,Ubogu EE

13-9700 was used in Immunocytochemistry to study the mechanisms of human blood-nerve barrier (BNB) formation and ascertain the downstream signalling pathway involved in the process. To determine the molecular changes that may occur at human BNB intercellular junctions under the influence of glial-derived neurotrophic factor (GDNF).

Mon Apr 01 00:00:00 EDT 2019

E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface.

Scientific reports

Advedissian T,Proux-Gillardeaux V,Nkosi R,Peyret G,Nguyen T,Poirier F,Viguier M,Deshayes F

13-9700 was used in Immunofluorescence to identify galectin-7 as a direct partner of E-cadherin, a key component of adherens junctions.

Wed Dec 06 00:00:00 EST 2017

The Human Blood-Nerve Barrier Transcriptome.

Scientific reports

Palladino SP,Helton ES,Jain P,Dong C,Crowley MR,Crossman DK,Ubogu EE

13-9700 was used in Immunohistochemistry to sequence the human blood-nerve barrier transcriptome using confluent P3 and P8 pHEndEC monolayers and normal adult laser-capture microdissected endoneurial microvessels.

Tue Dec 12 00:00:00 EST 2017

Chronic hypoxia down-regulates tight junction protein ZO-2 expression in children with cyanotic congenital heart defect.

ESC heart failure

Jenkins EL,Caputo M,Angelini GD,Ghorbel MT

Published figure using alpha Catenin monoclonal antibody (Product # 13-9700) in Western Blot

Wed Jun 01 00:00:00 EDT 2016

Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY

13-9700 was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis

Tue Sep 01 00:00:00 EDT 2015

Characterization of α-, β- and p120-Catenin Expression in Feline Mammary Tissues and their Relation with E- and P-Cadherin.

Anticancer research

Figueira AC,Gomes C,Vilhena H,Miranda S,Carvalheira J,DE Matos AJ,Dias-Pereira P,Gärtner F

13-9700 was used in immunohistochemistry (paraffin) to discuss catenin expression in feline mammary tissues.

Mon Jun 01 00:00:00 EDT 2015

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Endocrinology

Qian X,Mruk DD,Wong EW,Lie PP,Cheng CY

13-9700 was used in Immunofluorescence to show that palladin is an actin filament regulator at the ectoplasmic specialization.

Wed May 01 00:00:00 EDT 2013

Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.

PloS one

Qian X,Mruk DD,Cheng CY

13-9700 was used in immunohistochemistry to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle

Mon Sep 23 00:00:00 EDT 2013

Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.

PloS one

Qian X,Mruk DD,Cheng CY

13-9700 was used in immunohistochemistry to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle

Mon Sep 23 00:00:00 EDT 2013

Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.

PloS one

Qian X,Mruk DD,Cheng CY

13-9700 was used in immunohistochemistry to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle

Mon Sep 23 00:00:00 EDT 2013

α-Catenin contributes to the strength of E-cadherin-p120 interactions.

Molecular biology of the cell

Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

Tue Nov 01 00:00:00 EDT 2011

α-Catenin contributes to the strength of E-cadherin-p120 interactions.

Molecular biology of the cell

Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

Tue Nov 01 00:00:00 EDT 2011

Melanoma prognostic model using tissue microarrays and genetic algorithms.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Gould Rothberg BE,Berger AJ,Molinaro AM,Subtil A,Krauthammer MO,Camp RL,Bradley WR,Ariyan S,Kluger HM,Rimm DL

Tue Dec 01 00:00:00 EST 2009

Cell discohesion and multifocality of carcinoma in situ of the bladder: new insight from the adhesion molecule profile (e-cadherin, Ep-CAM, and MUC1).

International journal of surgical pathology

Patriarca C,Colombo P,Pio Taronna A,Wesseling J,Franchi G,Guddo F,Naspro R,Macchi RM,Giunta P,Di Pasquale M,Parente M,Arizzi C,Roncalli M,Campo B

13-9700 was used in immunohistochemistry - paraffin section to determine the expression of several adhesion molecules and MUC1 in samples from patients with unifocal or multifocal bladder urothelial cell carcinoma in situ

Wed Apr 01 00:00:00 EDT 2009

Localization of claudin-5 and ZO-1 in rat spleen sinus endothelial cells.

Histochemistry and cell biology

Uehara K,Uehara A

13-9700 was used in immunohistochemistry - frozen section to study the spatial and functional relationships between tight and adherens junctions in the sinus endothelial cells of rat spleen

Tue Jan 01 00:00:00 EST 2008

Expression of the E-cadherin-catenins complex in sentinel node is related to tumor morphology but not to spread to nonsentinel nodes.

Pathology, research and practice

Canavese G,Bernardi A,Candelaresi G,Lovadina P,Amerio S,Rossetti V,Rabagliati C,Berardengo E

13-9700 was used in immunohistochemistry - paraffin section to quantify the expression of E-cadherin and a- and b-catenins that form the cadherin-catenin complex in tumoral vascular invasion in metastatic sentinel lymph node patients

Fri Oct 12 00:00:00 EDT 2007

Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion.

The Journal of comparative neurology

Akins MR,Greer CA

13-9700 was used in immunohistochemistry to examine the involvement of catenin-cadherin mediated adhesion in the axonal projection of olfactory sensory neuron (OSN) axons

Wed Dec 20 00:00:00 EST 2006

Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.

Cancer research

Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F

Tue Feb 01 00:00:00 EST 2005

Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex.

Journal of cell science

Kim YJ,Sauer C,Testa K,Wahl JK,Svoboda RA,Johnson KR,Wheelock MJ,Knudsen KA

13-9700 was used in immunocytochemistry and western blot to identify and characterize a novel classical cadherin adhesion system

Thu Sep 01 00:00:00 EDT 2005

Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease).

Heart rhythm

Kaplan SR,Gard JJ,Protonotarios N,Tsatsopoulou A,Spiliopoulou C,Anastasakis A,Squarcioni CP,McKenna WJ,Thiene G,Basso C,Brousse N,Fontaine G,Saffitz JE

13-9700 was used in immunohistochemistry - frozen section to investigate the effect of the plakoglobin mutation in Naxos disease on connexin43

Sat May 01 00:00:00 EDT 2004

Immunohistochemical patterns for alpha- and beta-catenin, E- and N-cadherin expression in ovarian epithelial tumors.

Gynecologic oncology

Marques FR,Fonsechi-Carvasan GA,De Angelo Andrade LA,Böttcher-Luiz F

13-9700 was used in immunohistochemistry - paraffin section to measure the E- and N-cadherin and beta- and alpha-catenin expression in benign and malignant epithelial neoplasms of the ovary and correlate expression with tumor staging, histological grade,

Thu Jul 01 00:00:00 EDT 2004

Abnormal expression of E-cadherin in early gastric carcinoma: its relationship with macroscopic growth patterns and catenin alpha and beta.

Journal of clinical gastroenterology

Song SY,Kim S,Kim DS,Son HJ,Rhee JC,Kim YI

13-9700 was used in immunohistochemistry - paraffin section to elucidate the relation between the macroscopic growth patterns of gastric carcinoma and the abnormal expression of E-cadherin and alpha- and beta-catenins

Mon Mar 01 00:00:00 EST 2004

Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells.

Human molecular genetics

Scheffers MS,Le H,van der Bent P,Leonhard W,Prins F,Spruit L,Breuning MH,de Heer E,Peters DJ

Tue Jan 01 00:00:00 EST 2002

Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells.

Human molecular genetics

Scheffers MS,Le H,van der Bent P,Leonhard W,Prins F,Spruit L,Breuning MH,de Heer E,Peters DJ

Tue Jan 01 00:00:00 EST 2002

Progressive changes in adherens junction structure during intestinal adenoma formation in Apc mutant mice.

The Journal of biological chemistry

Carothers AM,Melstrom KA,Mueller JD,Weyant MJ,Bertagnolli MM

Fri Oct 19 00:00:00 EDT 2001

Monocytes induce reversible focal changes in vascular endothelial cadherin complex during transendothelial migration under flow.

The Journal of cell biology

Allport JR,Muller WA,Luscinskas FW

13-9700 was used in immunocytochemistry to propose that an endothelial-dependent step of transient and focal disruption of the vascular endothelial-cadherin complex occurs during leukocyte transmigration

Mon Jan 10 00:00:00 EST 2000

Changing roles of cadherins and catenins during progression of squamous intraepithelial lesions in the uterine cervix.

The American journal of pathology

de Boer CJ,van Dorst E,van Krieken H,Jansen-van Rhijn CM,Warnaar SO,Fleuren GJ,Litvinov SV

13-9700 was used in western blot to elucidate the transformation of normal squamous epithelium to squamous intraepithelial lesions using the uterine cervix.

Sun Aug 01 00:00:00 EDT 1999

Analysis of gene expression in human bullous keratopathy corneas containing limiting amounts of RNA.

Investigative ophthalmology & visual science

Spirin KS,Ljubimov AV,Castellon R,Wiedoeft O,Marano M,Sheppard D,Kenney MC,Brown DJ

13-9700 was used in immunohistochemistry (frozen) to validate the use of polymerase chain reaction-amplified full-length cDNA as a substitute for mRNA in nucleic acid array and gene expression analysis.

Wed Dec 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; Alpha N-catenin; alpha(E)-catenin; alpha(N)-catenin; alpha-catenin; Alpha-catenin-related protein; alpha-E-catenin; alpha-N-catenin; alphaE-catenin; alphaN-catenin; cadherin associated protein; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; CAP102; catenin; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; catenin alpha 1; catenin alpha 2; Catenin alpha-1; Catenin alpha-2; Catenin alpha1; Catenin alpha2; catenin, alpha 1; Catenin-alpha1; Catenin-alpha2; cerebellar deficient folia; FLJ36832; FLJ52416; HGNC:2509; Neural alpha-catenin; NY-REN-13 antigen; Renal carcinoma antigen NY-REN-13

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Gene Aliases: 2010010M04Rik; AA517462; AI481747; AI988031; CAP-R; CAP102; CAPR; Catna; Catna1; Catna2; cdf; chp; CT114; CTNNA1; CTNNA2; CTNR; MDPT2

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UniProt ID: (Human) P35221, (Human) P26232, (Chicken) P30997, (Mouse) P26231, (Mouse) Q61301

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Entrez Gene ID: (Human) 1495, (Chicken) 416184, (Human) 1496, (Chicken) 396035, (Rat) 307505, (Mouse) 12385, (Mouse) 12386, (Rat) 297357

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Function(s)

structural molecule activity protein binding beta-catenin binding vinculin binding poly(A) RNA binding gamma-catenin binding cadherin binding actin filament binding cadherin binding involved in cell-cell adhesion structural constituent of cytoskeleton non-motor actin binding protein

Process(es)

ovarian follicle development actin filament organization cell adhesion establishment or maintenance of cell polarity negative regulation of neuroblast proliferation aging male gonad development gap junction assembly axon regeneration adherens junction organization cellular protein localization odontogenesis of dentin-containing tooth apical junction assembly response to estrogen positive regulation of smoothened signaling pathway positive regulation of muscle cell differentiation protein heterooligomerization cellular response to indole-3-methanol epithelial cell-cell adhesion negative regulation of cell motility negative regulation of integrin-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of extrinsic apoptotic signaling pathway in absence of ligand cytoskeleton organization axonogenesis single organismal cell-cell adhesion radial glia guided migration of Purkinje cell dendrite morphogenesis brain morphogenesis regulation of synapse structural plasticity prepulse inhibition neural crest cell migration protein localization epithelial cell differentiation trigeminal ganglion formation cell-cell adhesion response to organic cyclic compound regulation of cell proliferation negative regulation of apoptotic process multicellular organism development cell differentiation

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alpha Catenin Monoclonal Antibody (alpha-CAT-7A4)

alpha Catenin Antibody (13-9700)

Product Details

13-9700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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