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alpha Internexin Antibody (PA1-10017) in ICC/IF
Immunofluorescent analysis of alpha internexin in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:2,000 as seen in green, using a GFAP polyclonal ant... View More
Product Details
PA1-10017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified, recombinant rat alpha-internexin, expressed in E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2127487
Product Specific Information
In Western blot, a prominent band at ~66 kDa is observed, as well as smaller lower bands which are apparently degradation products. A minor band at ~150 kDa is also seen, resulting from dimerization of alpha-internexin.
Target Information
alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; NF-66
Gene Aliases: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1
UniProt ID: (Human) Q16352, (Bovine) Q08DH7, (Mouse) P46660
Entrez Gene ID: (Human) 9118, (Pig) 100153400, (Horse) 100059769, (Bovine) 532236, (Mouse) 226180, (Rat) 24503
multicellular organism development
nervous system development
cell differentiation
intermediate filament cytoskeleton organization
neurofilament cytoskeleton organization
substantia nigra development
tissue regeneration
postsynaptic actin cytoskeleton organization
postsynaptic intermediate filament cytoskeleton organization
cellular response to leukemia inhibitory factor
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