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alpha Internexin Antibody (PA1-4737) in ICC/IF
Immunocytochemistry analysis of alpha Internexin in neurons and glia cells showing specific labeling of neuronal processes with alpha Internexin polyclonal antibody (Product # PA1-4737) with a dilution of 1:500 (red), and specific labeling of microglia with Anti-Coronin 1a (green).
Product Details
PA1-4737
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant rat alpha-internexin expressed in and purified from E. coli
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
IgY fraction
Storage buffer
PBS
Contains
10mM sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
RRID
AB_10977046
Product Specific Information
In Western blot, a band at ~66 kDa is detected. Minor bands of approximately 50 kDa and 150 kDa are also detected, which most likely correspond to alpha-internexin breakdown products and covalent dimers, respectively.
Target Information
alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; NF-66
Gene Aliases: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1
UniProt ID: (Human) Q16352, (Bovine) Q08DH7, (Mouse) P46660
Entrez Gene ID: (Human) 9118, (Bovine) 532236, (Mouse) 226180, (Rat) 24503
multicellular organism development
nervous system development
cell differentiation
intermediate filament cytoskeleton organization
neurofilament cytoskeleton organization
substantia nigra development
tissue regeneration
postsynaptic actin cytoskeleton organization
postsynaptic intermediate filament cytoskeleton organization
cellular response to leukemia inhibitory factor
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