Invitrogen

alpha Internexin Monoclonal Antibody (2E3)

alpha Internexin Antibody in Immunocytochemistry (ICC/IF)

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alpha Internexin Antibody (MA3-16726) in ICC/IF

Immunocytochemistry analysis of alpha Internexin in Hippocampal neurons in tissue culture. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726). Neurofilament alpha internexin (red), using alpha-Internexin Antibody and GFAP antibody (green).

alpha Internexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Internexin Antibody in Western Blot (WB)

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Product Details

MA3-16726

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:5,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:5,000

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E3

Immunogen

Purified recombinant rat a-internexin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_568590

Product Specific Information

The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis.

Human mutation

van der Zee J,Gijselinck I,Van Mossevelde S,Perrone F,Dillen L,Heeman B,Bäumer V,Engelborghs S,De Bleecker J,Baets J,Gelpi E,Rojas-García R,Clarimón J,Lleó A,Diehl-Schmid J,Alexopoulos P,Perneczky R,Synofzik M,Just J,Schöls L,Graff C,Thonberg H,Borroni B,Padovani A,Jordanova A,Sarafov S,Tournev I,de Mendonça A,Miltenberger-Miltényi G,Simões do Couto F,Ramirez A,Jessen F,Heneka MT,Gómez-Tortosa E,Danek A,Cras P,Vandenberghe R,De Jonghe P,De Deyn PP,Sleegers K,Cruts M,Van Broeckhoven C,Goeman J,Nuytten D,Smets K,Robberecht W,Damme PV,Bleecker J,Santens P,Dermaut B,Versijpt J,Michotte A,Ivanoiu A,Deryck O,Bergmans B,Delbeck J,Bruyland M,Willems C,Salmon E,Pastor P,Ortega-Cubero S,Benussi L,Ghidoni R,Binetti G,Hernández I,Boada M,Ruiz A,Sorbi S,Nacmias B,Bagnoli S,Sorbi S,Sanchez-Valle R,Llado A,Santana I,Rosário Almeida M,Frisoni GB,Maetzler W,Matej R,Fraidakis MJ,Kovacs GG,Fabrizi GM,Testi S

MA316726 was used in immunohistochemistry - paraffin section to examine the mutation spectrum of the TANK-Binding Kinase 1 gene and its associated phenotypic spectrum in patients with FTD, ALS or FTD plus ALS

Wed Mar 01 00:00:00 EST 2017

Neuropathological criteria of anti-IgLON5-related tauopathy.

Acta neuropathologica

Gelpi E,Höftberger R,Graus F,Ling H,Holton JL,Dawson T,Popovic M,Pretnar-Oblak J,Högl B,Schmutzhard E,Poewe W,Ricken G,Santamaria J,Dalmau J,Budka H,Revesz T,Kovacs GG

MA316726 was used in immunohistochemistry - paraffin section to detail the neuropathological features of the anti-IgLON5 syndrome in order to diagnose patients

Sat Oct 01 00:00:00 EDT 2016

An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions.

Neuropathology : official journal of the Japanese Society of Neuropathology

Inoue K,Fujimura H,Ueda K,Matsumura T,Itoh K,Sakoda S

MA3-16726 was used in immunohistochemistry - paraffin section to report an autopsy case of neuronal intermediate filament inclusion disease.

Tue Dec 01 00:00:00 EST 2015

Cytoplasmic non-epithelial mucin accumulation associated with CD44 in an astrocytic tumor with signet ring features.

Brain tumor pathology

Okabe H,Nagata A,Katsura K,Ishida M,Osaka Y,Tenjin H

MA3-16726 was used in immunohistochemistry to report on a case of an unusual astrocytic tumor rich in signet ring cells

Tue Apr 01 00:00:00 EDT 2014

Gliosarcoma with ependymal and PNET-like differentiation.

Clinical neuropathology

Shintaku M,Yoneda H,Hirato J,Nagaishi M,Okabe H

MA3-16726 was used in immunohistochemistry to characterize a rare case of gliosarcoma

Thu Mar 13 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; NF-66

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Gene Aliases: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1

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UniProt ID: (Human) Q16352, (Bovine) Q08DH7, (Mouse) P46660

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Entrez Gene ID: (Human) 9118, (Pig) 100153400, (Bovine) 532236, (Mouse) 226180, (Rat) 24503

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Function(s)

structural constituent of cytoskeleton structural constituent of postsynaptic actin cytoskeleton structural constituent of postsynaptic intermediate filament cytoskeleton structural molecule activity protein binding protein heterodimerization activity

Process(es)

multicellular organism development nervous system development cell differentiation intermediate filament cytoskeleton organization neurofilament cytoskeleton organization substantia nigra development tissue regeneration postsynaptic actin cytoskeleton organization postsynaptic intermediate filament cytoskeleton organization cellular response to leukemia inhibitory factor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Internexin Monoclonal Antibody (2E3)

alpha Internexin Antibody (MA3-16726) in ICC/IF

Product Details

MA3-16726

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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