Invitrogen

alpha Synuclein Monoclonal Antibody (10H7), FITC

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Protocols
Questions & Answers
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Product Details

MA5-45841

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

1:1,000
-

Dot blot (DB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H7

Immunogen

Mouse alpha synuclein aggregate
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932295

Product Specific Information

A 1:1,000 dilution of MA5-45841 was sufficient for detection of Alpha Synuclein in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Binds within the 101-130 aa region of human alpha synuclein, determined using Dot blot and ELISA with corresponding peptides. Detects approximately 14 kDa band corresponding to Alpha synuclein. Band at approximately 40 kDa is a trimer. Does not detect truncated alpha Synuclein (aa 1-114)

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

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Gene Aliases: alphaSYN; NACP; PARK1; PARK4; PD1; SNCA; Syn

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UniProt ID: (Human) P37840, (Rat) P37377, (Mouse) O55042

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Entrez Gene ID: (Human) 6622, (Rat) 29219, (Mouse) 20617

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Function(s)
magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity enzyme binding Hsp70 protein binding arachidonic acid binding metal ion binding membrane trafficking regulatory protein
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of receptor recycling negative regulation of protein phosphorylation positive regulation of neurotransmitter secretion fatty acid metabolic process neutral lipid metabolic process phospholipid metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process mitochondrial membrane organization aging adult locomotory behavior response to iron(II) ion regulation of phospholipase activity negative regulation of platelet-derived growth factor receptor signaling pathway regulation of glutamate secretion regulation of dopamine secretion negative regulation of microtubule polymerization receptor internalization protein destabilization response to magnesium ion negative regulation of transporter activity response to lipopolysaccharide negative regulation of monooxygenase activity positive regulation of peptidyl-serine phosphorylation response to interferon-gamma cellular response to oxidative stress negative regulation of histone acetylation regulation of locomotion dopamine biosynthetic process response to drug mitochondrial ATP synthesis coupled electron transport regulation of macrophage activation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process extracellular fibril organization negative regulation of neuron apoptotic process cellular protein metabolic process cellular response to fibroblast growth factor stimulus positive regulation of endocytosis negative regulation of exocytosis negative regulation of dopamine metabolic process behavioral response to cocaine regulation of long-term neuronal synaptic plasticity synaptic vesicle endocytosis synapse organization regulation of acyl-CoA biosynthetic process positive regulation of release of sequestered calcium ion into cytosol dopamine uptake involved in synaptic transmission negative regulation of dopamine uptake involved in synaptic transmission negative regulation of serotonin uptake negative regulation of norepinephrine uptake calcium ion homeostasis oxidation-reduction process excitatory postsynaptic potential long-term synaptic potentiation positive regulation of inositol phosphate biosynthetic process negative regulation of thrombin receptor signaling pathway response to interleukin-1 cellular response to copper ion cellular response to epinephrine stimulus positive regulation of protein serine/threonine kinase activity negative regulation of neuron death negative regulation of mitochondrial electron transport, NADH to ubiquinone positive regulation of glutathione peroxidase activity positive regulation of hydrogen peroxide catabolic process regulation of synaptic vesicle recycling regulation of reactive oxygen species biosynthetic process negative regulation of chaperone-mediated autophagy synaptic transmission, dopaminergic chemical synaptic transmission response to cocaine dopamine metabolic process regulation of neuron apoptotic process regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity synaptic vesicle transport positive regulation of synaptic transmission membrane organization regulation of neuron death regulation of reactive oxygen species metabolic process
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