Invitrogen

alpha Synuclein Monoclonal Antibody (4B12)

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Antibody Testing Data (1)
Published Figures (13)

alpha Synuclein Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 14

alpha Synuclein Antibody (MA1-90346) in IHC

Immunohistochemistry analysis of the cortical Lewy body using alpha Synuclein Monoclonal Antibody (4B12) (Product # MA1-90346).

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

alpha Synuclein Antibody in Western Blot, Immunohistochemistry (WB, IHC)

alpha Synuclein Antibody in Western Blot (WB)

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Product Details

MA1-90346

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:10,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Dot blot (DB)

1:100-1:10,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4B12

Immunogen

Recombinant human alpha synuclein produced in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1954821

Product Specific Information

Predicted molecular weight of 16 kDa.

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alpha-synuclein-associated changes in PINK1-PRKN-mediated mitophagy are disease context dependent.

Brain pathology (Zurich, Switzerland)

Hou X,Chen TH,Koga S,Bredenberg JM,Faroqi AH,Delenclos M,Bu G,Wszolek ZK,Carr JA,Ross OA,McLean PJ,Murray ME,Dickson DW,Fiesel FC,Springer W

MA1-90346 was used in Immunohistochemistry to suggest that pS65-Ub may be useful as a biomarker to discriminate different synucleinopathies and may serve as a potential therapeutic target for Lewy body disease.

Fri Sep 01 00:00:00 EDT 2023

Human pericytes degrade diverse α-synuclein aggregates.

PloS one

Dieriks BV,Highet B,Alik A,Bellande T,Stevenson TJ,Low V,Park TI,Correia J,Schweder P,Faull RLM,Melki R,Curtis MA,Dragunow M

MA1-90346 was used in Dot Blot, ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to highlight the role brain vasculature may play in reducing α-syn aggregate burden in Parkinson's disease.

Tue Nov 22 00:00:00 EST 2022

Human pericytes degrade diverse α-synuclein aggregates.

PloS one

Dieriks BV,Highet B,Alik A,Bellande T,Stevenson TJ,Low V,Park TI,Correia J,Schweder P,Faull RLM,Melki R,Curtis MA,Dragunow M

Tue Nov 22 00:00:00 EST 2022

α-synuclein buildup is alleviated via ESCRT-dependent endosomal degradation brought about by p38MAPK inhibition in cells expressing p25α.

The Journal of biological chemistry

Borland H,Rasmussen I,Bjerregaard-Andersen K,Rasmussen M,Olsen A,Vilhardt F

MA1-90346 was used in Sample Preparation to suggest that under conditions of autophagolysosomal malfunction, the uninterrupted endosomal pathway offers a possibility to achieve disease-associated protein degradation.

Tue Nov 01 00:00:00 EDT 2022

Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy.

NPJ Parkinson's disease

Fanning S,Cirka H,Thies JL,Jeong J,Niemi SM,Yoon J,Ho GPH,Pacheco JA,Dettmer U,Liu L,Clish CB,Hodgetts KJ,Hutchinson JN,Muratore CR,Caldwell GA,Caldwell KA,Selkoe D

MA1-90346 was used in Western Blotting to identify the rate-limiting lipase enzyme, LIPE, as a candidate target.

Thu Jun 09 00:00:00 EDT 2022

The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability.

Cell

Hallacli E,Kayatekin C,Nazeen S,Wang XH,Sheinkopf Z,Sathyakumar S,Sarkar S,Jiang X,Dong X,Di Maio R,Wang W,Keeney MT,Felsky D,Sandoe J,Vahdatshoar A,Udeshi ND,Mani DR,Carr SA,Lindquist S,De Jager PL,Bartel DP,Myers CL,Greenamyre JT,Feany MB,Sunyaev SR,Chung CY,Khurana V

MA1-90346 was used in Dot Blot, ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to show that αS directly modulates processing bodies (P-bodies), membraneless organelles that function in mRNA turnover and storage.

Thu Jun 09 00:00:00 EDT 2022

Clinicopathological Staging of Dynamics of Neurodegeneration and Neuronal Loss in Alzheimer Disease.

Journal of neuropathology and experimental neurology

Wegiel J,Flory M,Kuchna I,Nowicki K,Ma SY,Wegiel J,Badmaev E,Leon M,Wisniewski T,Reisberg B

MA1-90346 was used in Immunohistochemistry to establish the pattern of neurodegeneration, and the severity and rate of neuronal loss in mild cognitive impairment/mild Alzheimer's disease.

Fri Jan 01 00:00:00 EST 2021

Altered conformation of α-synuclein drives dysfunction of synaptic vesicles in a synaptosomal model of Parkinson's disease.

Cell reports

Fonseca-Ornelas L,Viennet T,Rovere M,Jiang H,Liu L,Nuber S,Ericsson M,Arthanari H,Selkoe DJ

MA1-90346 was used in Western Blot to conclude that the synaptic microenvironment is necessary for αSyn to reach its native conformations and establish a physiological interaction network.

Tue Jul 06 00:00:00 EDT 2021

Altered conformation of α-synuclein drives dysfunction of synaptic vesicles in a synaptosomal model of Parkinson's disease.

Cell reports

Fonseca-Ornelas L,Viennet T,Rovere M,Jiang H,Liu L,Nuber S,Ericsson M,Arthanari H,Selkoe DJ

MA1-90346 was used in Western Blot to conclude that the synaptic microenvironment is necessary for αSyn to reach its native conformations and establish a physiological interaction network.

Tue Jul 06 00:00:00 EDT 2021

Establishment of a human induced pluripotent stem cell neuronal model for identification of modulators of A53T α-synuclein levels and aggregation.

PloS one

Vajhøj C,Schmid B,Alik A,Melki R,Fog K,Holst B,Stummann TC

Published figure using alpha Synuclein monoclonal antibody (Product # MA1-90346) in Western Blot

Tue Jan 11 00:00:00 EST 2022

Nilotinib Fails to Prevent Synucleinopathy and Cell Loss in a Mouse Model of Multiple System Atrophy.

Movement disorders : official journal of the Movement Disorder Society

Lopez-Cuina M,Guerin PA,Canron MH,Delamarre A,Dehay B,Bezard E,Meissner WG,Fernagut PO

MA1-90346 was used in Western Blotting to suggest that partial inhibition of c-Abl and reduction of α-synuclein phosphorylation at tyrosine 39 may not be a relevant target for MSA.

Wed Jul 01 00:00:00 EDT 2020

A prolyl oligopeptidase inhibitor, KYP-2047, reduces α-synuclein protein levels and aggregates in cellular and animal models of Parkinson's disease.

British journal of pharmacology

Myöhänen TT,Hannula MJ,Van Elzen R,Gerard M,Van Der Veken P,García-Horsman JA,Baekelandt V,Männistö PT,Lambeir AM

MA1-90346 was used in immunocytochemistry, immunohistochemistry, and western blot to study the reduction in the levels of alpha-synuclein protein and aggregation by a prolyl peptidase inhibitor in various models of Parkinson's disease

Fri Jun 01 00:00:00 EDT 2012

A prolyl oligopeptidase inhibitor, KYP-2047, reduces α-synuclein protein levels and aggregates in cellular and animal models of Parkinson's disease.

British journal of pharmacology

Myöhänen TT,Hannula MJ,Van Elzen R,Gerard M,Van Der Veken P,García-Horsman JA,Baekelandt V,Männistö PT,Lambeir AM

Fri Jun 01 00:00:00 EDT 2012

A prolyl oligopeptidase inhibitor, KYP-2047, reduces α-synuclein protein levels and aggregates in cellular and animal models of Parkinson's disease.

British journal of pharmacology

Myöhänen TT,Hannula MJ,Van Elzen R,Gerard M,Van Der Veken P,García-Horsman JA,Baekelandt V,Männistö PT,Lambeir AM

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

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Gene Aliases: NACP; PARK1; PARK4; PD1; SNCA

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UniProt ID: (Human) P37840

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Entrez Gene ID: (Human) 6622

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Function(s)

magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity membrane trafficking regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Synuclein Monoclonal Antibody (4B12)

alpha Synuclein Antibody (MA1-90346) in IHC

Product Details

MA1-90346

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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