Invitrogen

alpha Synuclein Monoclonal Antibody (Syn 211)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

33 Published Figures

54 References

View all (84) alpha Synuclein antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

alpha Synuclein Antibody in Western Blot (WB)

FIGURE: 1 / 38

alpha Synuclein Antibody (32-8100) in WB

Western blot was performed using Anti-alpha Synuclein Monoclonal Antibody (Syn 211) (Product # 32-8100) and a 14 kDa band corresponding to alpha Synuclein was observed across the cell lines tested. Membrane enriched extracts (30 µg lysate) of HEK-293 (Lane 1), HEL 92.1.7 (Lane 2), Hep G2 (Lane 3), A-549 (Lane 4), Jurkat (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Produ... View More

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

alpha Synuclein Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

alpha Synuclein Antibody in Immunohistochemistry (Frozen) (IHC (F))

alpha Synuclein Antibody in Immunohistochemistry (IHC)

alpha Synuclein Antibody in Western Blot, Immunohistochemistry (WB, IHC)

alpha Synuclein Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

alpha Synuclein Antibody in Western Blot, Dot Blot (WB, DB)

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Product Details

32-8100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 9 publications 9 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 2 publications 2 publications

Dot blot (DB)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Syn 211

Immunogen

Recombinant human alpha-synuclein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533094

Product Specific Information

This antibody reacts with the alpha-synuclein protein. This antibody does not cross-react with beta- or gamma-synuclein or synoretin. During development reactivity was confirmed with human. Reactivity with other species has not been tested.

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cortical Lewy body injections induce long-distance pathogenic alterations in the non-human primate brain.

NPJ Parkinson's disease

Teil M,Dovero S,Bourdenx M,Arotcarena ML,Darricau M,Porras G,Thiolat ML,Trigo-Damas I,Perier C,Estrada C,Garcia-Carrillo N,Herrero MT,Vila M,Obeso JA,Bezard E,Dehay B

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Tue Sep 19 00:00:00 EDT 2023

TDP-43 and tau concurrence in the entorhinal subfields in primary age-related tauopathy and preclinical Alzheimer's disease.

Brain pathology (Zurich, Switzerland)

Llamas-Rodríguez J,Oltmer J,Marshall M,Champion S,Frosch MP,Augustinack JC

32-8100 was used in Immunohistochemistry to highlight pTDP-43 burden heterogeneity within the EC and the posterior-lateral subfields as the most vulnerable regions within stage II of the current pTDP-43 staging schemes for AD and PART.

Sat Jul 01 00:00:00 EDT 2023

Mercury is present in neurons and oligodendrocytes in regions of the brain affected by Parkinson's disease and co-localises with Lewy bodies.

PloS one

Pamphlett R,Bishop DP

32-8100 was used in Immunohistochemistry to compare the cellular distribution of mercury in the tissues of people with and without PD who had evidence of previous mercury exposure by mercury being present in their locus ceruleus neurons.

Mon Feb 21 00:00:00 EST 2022

Mercury is present in neurons and oligodendrocytes in regions of the brain affected by Parkinson's disease and co-localises with Lewy bodies.

PloS one

Pamphlett R,Bishop DP

Mon Feb 21 00:00:00 EST 2022

Rapid macropinocytic transfer of α-synuclein to lysosomes.

Cell reports

Bayati A,Banks E,Han C,Luo W,Reintsch WE,Zorca CE,Shlaifer I,Del Cid Pellitero E,Vanderperre B,McBride HM,Fon EA,Durcan TM,McPherson PS

32-8100 was used in Western Blotting to demonstrate that alpha-synuclein fibrils undergoes rapid internalization and is targeted directly to lysosomes.

Tue Jul 19 00:00:00 EDT 2022

Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.

STAR protocols

Stojkovska I,Mazzulli JR

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Immunocytochemistry

Fri Mar 19 00:00:00 EDT 2021

Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.

STAR protocols

Stojkovska I,Mazzulli JR

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Immunocytochemistry

Fri Mar 19 00:00:00 EDT 2021

Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.

STAR protocols

Stojkovska I,Mazzulli JR

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Immunocytochemistry

Fri Mar 19 00:00:00 EDT 2021

Small molecule inhibitors of α-synuclein oligomers identified by targeting early dopamine-mediated motor impairment in C. elegans.

Molecular neurodegeneration

Chen KS,Menezes K,Rodgers JB,O'Hara DM,Tran N,Fujisawa K,Ishikura S,Khodaei S,Chau H,Cranston A,Kapadia M,Pawar G,Ping S,Krizus A,Lacoste A,Spangler S,Visanji NP,Marras C,Majbour NK,El-Agnaf OMA,Lozano AM,Culotti J,Suo S,Ryu WS,Kalia SK,Kalia LV

32-8100 was used in ELISA, Immunohistochemistry, Western Blot, Immunoprecipitation to identify a C. elegans locomotor abnormality due to dopaminergic neuron dysfunction that models early α-synuclein-mediated neurodegeneration.

Fri Nov 12 00:00:00 EST 2021

Small molecule inhibitors of α-synuclein oligomers identified by targeting early dopamine-mediated motor impairment in C. elegans.

Molecular neurodegeneration

Fri Nov 12 00:00:00 EST 2021

Small molecule inhibitors of α-synuclein oligomers identified by targeting early dopamine-mediated motor impairment in C. elegans.

Molecular neurodegeneration

Fri Nov 12 00:00:00 EST 2021

Viral alpha-synuclein knockdown prevents spreading synucleinopathy.

Brain communications

Menon S,Kofoed RH,Nabbouh F,Xhima K,Al-Fahoum Y,Langman T,Mount HTJ,Shihabuddin LS,Sardi SP,Fraser PE,Watts JC,Aubert I,Tandon A

32-8100 was used in Immunohistochemistry to test whether silencing α-syn gene expression reduces native non-aggregated α-syn substrate and thereby disrupt the propagation of pathological α-syn initiated by seeding with synucleinopathy-affected mouse brain homogenates.

Fri Nov 12 00:00:00 EST 2021

Spinal Cord and Motor Neuron TDP-43 Pathology in a Sporadic Inclusion Body Myositis Patient.

Journal of neuropathology and experimental neurology

Cathcart SJ,Greene EP,Powell SZ,Arumanayagam AS,Rivera AL,Tawil R,Appel SH,Cykowski MD

32-8100 was used in Immunohistochemistry to report detailed description of spinal cord pathology in sporadic IBM(sIBM), including ventral nerve root atrophy, chromatolytic neurons, and rare motor neurons with p62 and pTDP-43 inclusions.

Thu Oct 01 00:00:00 EDT 2020

Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys.

EBioMedicine

Alarcón-Arís D,Pavia-Collado R,Miquel-Rio L,Coppola-Segovia V,Ferrés-Coy A,Ruiz-Bronchal E,Galofré M,Paz V,Campa L,Revilla R,Montefeltro A,Kordower JH,Vila M,Artigas F,Bortolozzi A

32-8100 was used in Western Blotting to disrupt the α-synuclein mRNA transcription selectively in monoamine neurons of a PD-like mouse model and elderly nonhuman primates. This is to see if the inhibition of α-synuclein production in dopamine neurons and its accumulation in cortical/striatal projection areas may alleviate the early deficient dopamine function.

Tue Sep 01 00:00:00 EDT 2020

Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys.

EBioMedicine

Alarcón-Arís D,Pavia-Collado R,Miquel-Rio L,Coppola-Segovia V,Ferrés-Coy A,Ruiz-Bronchal E,Galofré M,Paz V,Campa L,Revilla R,Montefeltro A,Kordower JH,Vila M,Artigas F,Bortolozzi A

Tue Sep 01 00:00:00 EDT 2020

Recapitulating Parkinson's disease pathology in a three-dimensional human neural cell culture model.

Disease models & mechanisms

Taylor-Whiteley TR,Le Maitre CL,Duce JA,Dalton CF,Smith DP

32-8100 was used in Immunocytochemistry-immunoflourescence to demonstrate that fully differentiated human SH-SY5Y neuroblastoma cells grown in three-dimensional cell culture develop Lewy-body-like pathology upon exposure to exogenous α-synuclein species.

Tue Apr 09 00:00:00 EDT 2019

Recapitulating Parkinson's disease pathology in a three-dimensional human neural cell culture model.

Disease models & mechanisms

Taylor-Whiteley TR,Le Maitre CL,Duce JA,Dalton CF,Smith DP

Tue Apr 09 00:00:00 EDT 2019

Alpha-Synuclein Continues to Enhance SNARE-Dependent Vesicle Docking at Exorbitant Concentrations.

Frontiers in neuroscience

Hawk BJD,Khounlo R,Shin YK

32-8100 was used in Western Blotting to assess the impact of α-synuclein on each step of membrane fusion.

Wed Sep 30 00:00:00 EDT 2020

Increased vulnerability of nigral dopamine neurons after expansion of their axonal arborization size through D2 dopamine receptor conditional knockout.

PLoS genetics

Giguère N,Delignat-Lavaud B,Herborg F,Voisin A,Li Y,Jacquemet V,Anand-Srivastava M,Gether U,Giros B,Trudeau LÉ

32-8100 was used in Immunohistochemistry to selectively label SNc or VTA DA neurons using floxed YFP viral injections in DAT-cre mice and show that SNc DA neurons have a much more arborized axon than those of the VTA.

Thu Aug 01 00:00:00 EDT 2019

Reversible Conformational Conversion of α-Synuclein into Toxic Assemblies by Glucosylceramide.

Neuron

Zunke F,Moise AC,Belur NR,Gelyana E,Stojkovska I,Dzaferbegovic H,Toker NJ,Jeon S,Fredriksen K,Mazzulli JR

32-8100 was used in Dot blot to determine the mechanism of glycosphin-golipid-induced aggregation.

Wed Jan 03 00:00:00 EST 2018

Autophagy inhibition promotes SNCA/alpha-synuclein release and transfer via extracellular vesicles with a hybrid autophagosome-exosome-like phenotype.

Autophagy

Minakaki G,Menges S,Kittel A,Emmanouilidou E,Schaeffner I,Barkovits K,Bergmann A,Rockenstein E,Adame A,Marxreiter F,Mollenhauer B,Galasko D,Buzás EI,Schlötzer-Schrehardt U,Marcus K,Xiang W,Lie DC,Vekrellis K,Masliah E,Winkler J,Klucken J

32-8100 was used in Immunohistochemistry to examine the mechanism by which the autophagy-lysosome pathway influences SNCA release.

Tue Feb 26 00:00:00 EST 2019

Noninvasive delivery of an α-synuclein gene silencing vector with magnetic resonance-guided focused ultrasound.

Movement disorders : official journal of the Movement Disorder Society

Xhima K,Nabbouh F,Hynynen K,Aubert I,Tandon A

32-8100 was used in Immunohistochemistry (PFA) to demonstrate that magnetic resonance-guided focused ultrasound can effectively, noninvasively, and simultaneously deliver viral vectors targeting α-synuclein to multiple brain areas.

Mon Oct 01 00:00:00 EDT 2018

Pathophysiological Consequences of Neuronal α-Synuclein Overexpression: Impacts on Ion Homeostasis, Stress Signaling, Mitochondrial Integrity, and Electrical Activity.

Frontiers in molecular neuroscience

Tolö J,Taschenberger G,Leite K,Stahlberg MA,Spehlbrink G,Kues J,Munari F,Capaldi S,Becker S,Zweckstetter M,Dean C,Bähr M,Kügler S

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Deferiprone Rescues Behavioral Deficits Induced by Mild Iron Exposure in a Mouse Model of Alpha-Synuclein Aggregation.

Neuromolecular medicine

Carboni E,Tatenhorst L,Tönges L,Barski E,Dambeck V,Bähr M,Lingor P

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Dot blot

Fri Sep 01 00:00:00 EDT 2017

Deferiprone Rescues Behavioral Deficits Induced by Mild Iron Exposure in a Mouse Model of Alpha-Synuclein Aggregation.

Neuromolecular medicine

Carboni E,Tatenhorst L,Tönges L,Barski E,Dambeck V,Bähr M,Lingor P

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Dot blot

Fri Sep 01 00:00:00 EDT 2017

Deferiprone Rescues Behavioral Deficits Induced by Mild Iron Exposure in a Mouse Model of Alpha-Synuclein Aggregation.

Neuromolecular medicine

Carboni E,Tatenhorst L,Tönges L,Barski E,Dambeck V,Bähr M,Lingor P

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Dot blot

Fri Sep 01 00:00:00 EDT 2017

Cortical phosphorylated α-Synuclein levels correlate with brain wave spectra in Parkinson's disease.

Movement disorders : official journal of the Movement Disorder Society

Caviness JN,Lue LF,Hentz JG,Schmitz CT,Adler CH,Shill HA,Sabbagh MN,Beach TG,Walker DG

32-8100 was used in western blot to analyze the correlation between brain wave spectra in Parkinson's disease and cortical phosphorylated alpha-synuclein levels

Fri Jul 01 00:00:00 EDT 2016

α-Synuclein interferes with the ESCRT-III complex contributing to the pathogenesis of Lewy body disease.

Human molecular genetics

Spencer B,Kim C,Gonzalez T,Bisquertt A,Patrick C,Rockenstein E,Adame A,Lee SJ,Desplats P,Masliah E

32-8100 was used in Immunocytochemistry to examine the role of the endosomal sorting complex required for transport pathway in the propagation of α-synuclein.

Tue Mar 15 00:00:00 EDT 2016

α-Synuclein interferes with the ESCRT-III complex contributing to the pathogenesis of Lewy body disease.

Human molecular genetics

Spencer B,Kim C,Gonzalez T,Bisquertt A,Patrick C,Rockenstein E,Adame A,Lee SJ,Desplats P,Masliah E

32-8100 was used in Immunocytochemistry to examine the role of the endosomal sorting complex required for transport pathway in the propagation of α-synuclein.

Tue Mar 15 00:00:00 EDT 2016

Towards a Non-Human Primate Model of Alpha-Synucleinopathy for Development of Therapeutics for Parkinson's Disease: Optimization of AAV1/2 Delivery Parameters to Drive Sustained Expression of Alpha Synuclein and Dopaminergic Degeneration in Macaque.

PloS one

Koprich JB,Johnston TH,Reyes G,Omana V,Brotchie JM

328100 was used in immunohistochemistry - free floating to develop a non-human primate model of synucleinopathy using cynomolgus macaque

Thu Jul 06 00:00:00 EDT 2017

The loss of glucose-regulated protein 78 (GRP78) during normal aging or from siRNA knockdown augments human alpha-synuclein (α-syn) toxicity to rat nigral neurons.

Neurobiology of aging

Salganik M,Sergeyev VG,Shinde V,Meyers CA,Gorbatyuk MS,Lin JH,Zolotukhin S,Gorbatyuk OS

32-8100 was used in immunohistochemistry - frozen section to determine if glucose-regulated protein 78 contributes to the development of Parkinson's disease.

Mon Jun 01 00:00:00 EDT 2015

The loss of glucose-regulated protein 78 (GRP78) during normal aging or from siRNA knockdown augments human alpha-synuclein (α-syn) toxicity to rat nigral neurons.

Neurobiology of aging

Salganik M,Sergeyev VG,Shinde V,Meyers CA,Gorbatyuk MS,Lin JH,Zolotukhin S,Gorbatyuk OS

32-8100 was used in immunohistochemistry - frozen section to determine if glucose-regulated protein 78 contributes to the development of Parkinson's disease.

Mon Jun 01 00:00:00 EDT 2015

Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse.

Acta neuropathologica communications

Henstridge CM,Jackson RJ,Kim JM,Herrmann AG,Wright AK,Harris SE,Bastin ME,Starr JM,Wardlaw J,Gillingwater TH,Smith C,McKenzie CA,Cox SR,Deary IJ,Spires-Jones TL

32-8100 was used in immunohistochemistry (paraffin) to present data from the first donated Lothian Birth Cohort of 1936 and compare it with other aged and diseased samples.

Fri Sep 04 00:00:00 EDT 2015

Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse.

Acta neuropathologica communications

Henstridge CM,Jackson RJ,Kim JM,Herrmann AG,Wright AK,Harris SE,Bastin ME,Starr JM,Wardlaw J,Gillingwater TH,Smith C,McKenzie CA,Cox SR,Deary IJ,Spires-Jones TL

32-8100 was used in immunohistochemistry (paraffin) to present data from the first donated Lothian Birth Cohort of 1936 and compare it with other aged and diseased samples.

Fri Sep 04 00:00:00 EDT 2015

Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse.

Acta neuropathologica communications

Henstridge CM,Jackson RJ,Kim JM,Herrmann AG,Wright AK,Harris SE,Bastin ME,Starr JM,Wardlaw J,Gillingwater TH,Smith C,McKenzie CA,Cox SR,Deary IJ,Spires-Jones TL

32-8100 was used in immunohistochemistry (paraffin) to present data from the first donated Lothian Birth Cohort of 1936 and compare it with other aged and diseased samples.

Fri Sep 04 00:00:00 EDT 2015

A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy.

Molecular neurodegeneration

Spencer B,Valera E,Rockenstein E,Trejo-Morales M,Adame A,Masliah E

32-8100 was used in western blot to reduce alpha-synuclein accumulation in a mouse model of multiple system atrophy by using a brain-targeted, modified neurosin (kallikrein-6)

Wed Sep 23 00:00:00 EDT 2015

A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy.

Molecular neurodegeneration

Spencer B,Valera E,Rockenstein E,Trejo-Morales M,Adame A,Masliah E

32-8100 was used in western blot to reduce alpha-synuclein accumulation in a mouse model of multiple system atrophy by using a brain-targeted, modified neurosin (kallikrein-6)

Wed Sep 23 00:00:00 EDT 2015

Hypoestoxide reduces neuroinflammation and α-synuclein accumulation in a mouse model of Parkinson's disease.

Journal of neuroinflammation

Kim C,Ojo-Amaize E,Spencer B,Rockenstein E,Mante M,Desplats P,Wrasidlo W,Adame A,Nchekwube E,Oyemade O,Okogun J,Chan M,Cottam H,Masliah E

32-8100 was used in immunohistochemistry to study a mouse model of Parkinson's disease for hypoestoxide to reduce neuroinflammation and alpha-synuclein accumulation

Fri Dec 18 00:00:00 EST 2015

Hypoestoxide reduces neuroinflammation and α-synuclein accumulation in a mouse model of Parkinson's disease.

Journal of neuroinflammation

Kim C,Ojo-Amaize E,Spencer B,Rockenstein E,Mante M,Desplats P,Wrasidlo W,Adame A,Nchekwube E,Oyemade O,Okogun J,Chan M,Cottam H,Masliah E

32-8100 was used in immunohistochemistry to study a mouse model of Parkinson's disease for hypoestoxide to reduce neuroinflammation and alpha-synuclein accumulation

Fri Dec 18 00:00:00 EST 2015

Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.

Cell death & disease

Koch JC,Bitow F,Haack J,d'Hedouville Z,Zhang JN,Tönges L,Michel U,Oliveira LM,Jovin TM,Liman J,Tatenhorst L,Bähr M,Lingor P

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Immunocytochemistry

Thu Jul 09 00:00:00 EDT 2015

Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.

Cell death & disease

Koch JC,Bitow F,Haack J,d'Hedouville Z,Zhang JN,Tönges L,Michel U,Oliveira LM,Jovin TM,Liman J,Tatenhorst L,Bähr M,Lingor P

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Immunocytochemistry

Thu Jul 09 00:00:00 EDT 2015

Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.

Acta neuropathologica communications

Bourdenx M,Dovero S,Engeln M,Bido S,Bastide MF,Dutheil N,Vollenweider I,Baud L,Piron C,Grouthier V,Boraud T,Porras G,Li Q,Baekelandt V,Scheller D,Michel A,Fernagut PO,Georges F,Courtine G,Bezard E,Dehay B

32-8100 was used in immunohistochemistry to study alpha-synuclein overexpression by lack of an additive role of aging in nigrostriatal neurodegeneration

Sat Jul 25 00:00:00 EDT 2015

Aggregation of αSynuclein promotes progressive in vivo neurotoxicity in adult rat dopaminergic neurons.

Acta neuropathologica

Taschenberger G,Garrido M,Tereshchenko Y,Bähr M,Zweckstetter M,Kügler S

Tue May 01 00:00:00 EDT 2012

Aggregation of αSynuclein promotes progressive in vivo neurotoxicity in adult rat dopaminergic neurons.

Acta neuropathologica

Taschenberger G,Garrido M,Tereshchenko Y,Bähr M,Zweckstetter M,Kügler S

Tue May 01 00:00:00 EDT 2012

Glucose regulated protein 78 diminishes α-synuclein neurotoxicity in a rat model of Parkinson disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gorbatyuk MS,Shabashvili A,Chen W,Meyers C,Sullivan LF,Salganik M,Lin JH,Lewin AS,Muzyczka N,Gorbatyuk OS

32-8100 was used in immunocytochemistry and western blot to test if BiP modulates the unfolded protein response, blocks apoptosis, and promotes the survival of nigral dopamine neurons using a rat model of Parkinson disease.

Sun Jul 01 00:00:00 EDT 2012

Glucose regulated protein 78 diminishes α-synuclein neurotoxicity in a rat model of Parkinson disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gorbatyuk MS,Shabashvili A,Chen W,Meyers C,Sullivan LF,Salganik M,Lin JH,Lewin AS,Muzyczka N,Gorbatyuk OS

Sun Jul 01 00:00:00 EDT 2012

Glucose regulated protein 78 diminishes α-synuclein neurotoxicity in a rat model of Parkinson disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gorbatyuk MS,Shabashvili A,Chen W,Meyers C,Sullivan LF,Salganik M,Lin JH,Lewin AS,Muzyczka N,Gorbatyuk OS

Sun Jul 01 00:00:00 EDT 2012

Glucose regulated protein 78 diminishes α-synuclein neurotoxicity in a rat model of Parkinson disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gorbatyuk MS,Shabashvili A,Chen W,Meyers C,Sullivan LF,Salganik M,Lin JH,Lewin AS,Muzyczka N,Gorbatyuk OS

Sun Jul 01 00:00:00 EDT 2012

Glucose regulated protein 78 diminishes α-synuclein neurotoxicity in a rat model of Parkinson disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gorbatyuk MS,Shabashvili A,Chen W,Meyers C,Sullivan LF,Salganik M,Lin JH,Lewin AS,Muzyczka N,Gorbatyuk OS

Sun Jul 01 00:00:00 EDT 2012

Sumoylation inhibits alpha-synuclein aggregation and toxicity.

The Journal of cell biology

Krumova P,Meulmeester E,Garrido M,Tirard M,Hsiao HH,Bossis G,Urlaub H,Zweckstetter M,Kügler S,Melchior F,Bähr M,Weishaupt JH

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Mon Jul 11 00:00:00 EDT 2011

Sumoylation inhibits alpha-synuclein aggregation and toxicity.

The Journal of cell biology

Krumova P,Meulmeester E,Garrido M,Tirard M,Hsiao HH,Bossis G,Urlaub H,Zweckstetter M,Kügler S,Melchior F,Bähr M,Weishaupt JH

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Mon Jul 11 00:00:00 EDT 2011

Neuroprotection by minocycline caused by direct and specific scavenging of peroxynitrite.

The Journal of biological chemistry

Schildknecht S,Pape R,Müller N,Robotta M,Marquardt A,Bürkle A,Drescher M,Leist M

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Fri Feb 18 00:00:00 EST 2011

Neuroprotection by minocycline caused by direct and specific scavenging of peroxynitrite.

The Journal of biological chemistry

Schildknecht S,Pape R,Müller N,Robotta M,Marquardt A,Bürkle A,Drescher M,Leist M

Published figure using alpha Synuclein monoclonal antibody (Product # 32-8100) in Western Blot

Fri Feb 18 00:00:00 EST 2011

Neuropathology in mice expressing mouse alpha-synuclein.

PloS one

Rieker C,Dev KK,Lehnhoff K,Barbieri S,Ksiazek I,Kauffmann S,Danner S,Schell H,Boden C,Ruegg MA,Kahle PJ,van der Putten H,Shimshek DR

32-8100 was used in western blot to characterize mice that over express murine alpha-Synuclein

Thu Mar 01 00:00:00 EST 2012

Hypocretin (orexin) loss in Parkinson's disease.

Brain : a journal of neurology

Fronczek R,Overeem S,Lee SY,Hegeman IM,van Pelt J,van Duinen SG,Lammers GJ,Swaab DF

32-8100 was used in immunohistochemistry - paraffin section to study the hypocretin system in patients with Parkinson's disease

Fri Jun 01 00:00:00 EDT 2007

Fine epitope mapping of monoclonal antibodies specific to human alpha-synuclein.

Neuroscience letters

Choi JY,Park HJ,Seong YM,Choi EY,Min BR,Rhim H

Thu May 25 00:00:00 EDT 2006

Fine epitope mapping of monoclonal antibodies specific to human alpha-synuclein.

Neuroscience letters

Choi JY,Park HJ,Seong YM,Choi EY,Min BR,Rhim H

Thu May 25 00:00:00 EDT 2006

Fine epitope mapping of monoclonal antibodies specific to human alpha-synuclein.

Neuroscience letters

Choi JY,Park HJ,Seong YM,Choi EY,Min BR,Rhim H

Thu May 25 00:00:00 EDT 2006

An in vitro model of Parkinson's disease: linking mitochondrial impairment to altered alpha-synuclein metabolism and oxidative damage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sherer TB,Betarbet R,Stout AK,Lund S,Baptista M,Panov AV,Cookson MR,Greenamyre JT

Thu Aug 15 00:00:00 EDT 2002

Effect of mutant alpha-synuclein on dopamine homeostasis in a new human mesencephalic cell line.

The Journal of biological chemistry

Lotharius J,Barg S,Wiekop P,Lundberg C,Raymon HK,Brundin P

Fri Oct 11 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

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Gene Aliases: NACP; PARK1; PARK4; PD1; SNCA

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UniProt ID: (Human) P37840

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Entrez Gene ID: (Human) 6622

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Function(s)

magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity membrane trafficking regulatory protein

Process(es)

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alpha Synuclein Monoclonal Antibody (Syn 211)

alpha Synuclein Antibody (32-8100) in WB

Product Details

32-8100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

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Type

Clone

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