Invitrogen

alpha Tubulin Polyclonal Antibody

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

alpha Tubulin Antibody (PA1-38814) in ICC/IF

Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA1-38814) at 1:250 dilution in 0.1% BSA and incubated... View More

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Tubulin Antibody in Western Blot (WB)

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Product Details

PA1-38814

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Mouse, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of humun tubulin alpha

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2210051

Product Specific Information

This antibody is predicted to react with mouse, bovine, guinea pig, porcine, gerbil, and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs.

Environmental toxicology and pharmacology

Elcombe CS,Monteiro A,Elcombe MR,Ghasemzadeh-Hasankolaei M,Sinclair KD,Lea R,Padmanabhan V,Evans NP,Bellingham M

PA1-38814 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide a potential mechanism for the pathogenesis of this testicular phenotype, and TDS in humans.

Mon Aug 01 00:00:00 EDT 2022

Anti-Semaphorin 4D Rescues Motor, Cognitive, and Respiratory Phenotypes in a Rett Syndrome Mouse Model.

International journal of molecular sciences

Mao Y,Evans EE,Mishra V,Balch L,Eberhardt A,Zauderer M,Gold WA

PA1-38814 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that anti-SEMA4D immunotherapy may be an effective treatment option to alleviate symptoms and improve cognitive and motor function in Rett syndrome.

Tue Aug 31 00:00:00 EDT 2021

A Probabilistic Boolean Network Approach for the Analysis of Cancer-Specific Signalling: A Case Study of Deregulated PDGF Signalling in GIST.

PloS one

Trairatphisan P,Wiesinger M,Bahlawane C,Haan S,Sauter T

PA1-38814 was used in western blot to utilize deregulated PDGF signaling in GIST to analyze cancer-specific signaling via a probabilistic boolean network to study cancer-specific signaling

Wed Jul 19 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B