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Invitrogen
alpha Tubulin Monoclonal Antibody (GT114)
FIGURE: 1 / 27
alpha Tubulin Antibody (MA5-31466) in ICC/IF
alpha Tubulin Polyclonal Antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:250. Red: NeuN, stained by NeuN Polyclonal Antibody (Product # P... View More
Product Details
MA5-31466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rat,
Zebrafish
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT114
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787098
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (95%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%).
Positive Control: 293T, A431, HeLa, HepG2, NIH-3T3, mouse brain, PC-12, rat brain, drosophila, zebrafish eye, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, U87-MG.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: &agr-tubulin 84B; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; AT25469p; beta-tubulin T beta15 (aa 1-445); CG1913-PA; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog; Tubulin-alpha1; tubulin1alpha; tubulin84B
Gene Aliases: 1t; 2410129E14Rik; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; Alpha-Tubulin84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; BEST:LD32507; brdp; cb944; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; fb22g06; fb40f09; H2-ALPHA; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; PMGYSA; RGD1309427; RGD1565476; T; Talpha1; TBA1_DROME; Tub; Tub 84B; Tub1C; Tub84B; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; TUBAL3; Tubalpha1; Tubb2; Tubb2a; TUBB2B; tubulin; wu:fb22g06; wu:fb37a10; wu:fb40f09; zgc:123194
UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) Q5XJF8, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P05216, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Fruit fly) P06603
Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081, (Fruit fly) 40848, (Zebrafish) 573216, (Zebrafish) 373080, (Zebrafish) 573122, (Zebrafish) 641421
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
protein domain specific binding
double-stranded RNA binding
ubiquitin protein ligase binding
molecular_function
enzyme binding
nucleotide binding
protein heterodimerization activity
tubulin
G2/M transition of mitotic cell cycle
cytoskeleton organization
microtubule-based process
cytoskeleton-dependent intracellular transport
cell division
microtubule cytoskeleton organization
cellular response to interleukin-4
biological_process
platelet degranulation
neuron migration
protein polymerization
metabolic process
mitotic cell cycle
lysosome localization
optic nerve formation
negative regulation of apoptotic process
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