Invitrogen

alpha Tubulin Monoclonal Antibody (YOL1/34)

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

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alpha Tubulin Antibody (MA1-80189) in ICC/IF

Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin (YOL1/34) Rat Monoclonal Antibody (Product # MA1-80189) at 2µg/mL in 0.1% BSA and incubated fo... View More

alpha Tubulin Antibody in Western Blot (WB)

alpha Tubulin Antibody in Immunoprecipitation (IP)

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Product Details

MA1-80189

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

2 µg/mL

View 4 publications 4 publications

ELISA (ELISA)

10 µg/mL as detecting antibody

Immunoprecipitation (IP)

View 1 publication 1 publication

Radioimmune Assays (RIA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Yeast

Published species

Fruit fly, Human, Mollusc, Mouse, Nematode, Xenopus, Yeast

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

YOL1/34

Immunogen

Yeast tubulin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2210200

Product Specific Information

Predicted to react with birds, fungi and all mammals based on sequence homology.

Rat anti Tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2.Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An affinity-directed phosphatase, AdPhosphatase, system for targeted protein dephosphorylation.

Cell chemical biology

Simpson LM,Fulcher LJ,Sathe G,Brewer A,Zhao JF,Squair DR,Crooks J,Wightman M,Wood NT,Gourlay R,Varghese J,Soares RF,Sapkota GP

MA1-80189 was used in Western Blotting to describe the development and application of the affinity-directed phosphatase (AdPhosphatase) system for targeted dephosphorylation of specific phospho-substrates.

Thu Feb 16 00:00:00 EST 2023

Localization of a TORC1-eIF4F translation complex during CD8⁺ T cell activation drives divergent cell fate.

Molecular cell

Liedmann S,Liu X,Guy CS,Crawford JC,Rodriguez DA,Kuzuoğlu-Öztürk D,Guo A,Verbist KC,Temirov J,Chen MJ,Ruggero D,Zhang H,Thomas PG,Green DR

MA1-80189 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Radioimmune assays, Western Blot to provide mechanistic insights as to how distinct T cell fate trajectories can be established during the first division.

Thu Jul 07 00:00:00 EDT 2022

Target protein localization and its impact on PROTAC-mediated degradation.

Cell chemical biology

Simpson LM,Glennie L,Brewer A,Zhao JF,Crooks J,Shpiro N,Sapkota GP

MA1-80189 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Radioimmune assays, Western Blot to suggest that the subcellular context of the POI can influence the efficacy of PROTAC-mediated POI degradation.

Thu Oct 20 00:00:00 EDT 2022

FAM83F regulates canonical Wnt signalling through an interaction with CK1α.

Life science alliance

Dunbar K,Jones RA,Dingwell K,Macartney TJ,Smith JC,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Mon Feb 01 00:00:00 EST 2021

IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.

Life science alliance

Dunbar K,Macartney TJ,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Mon Feb 01 00:00:00 EST 2021

IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.

Life science alliance

Dunbar K,Macartney TJ,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Mon Feb 01 00:00:00 EST 2021

LEA motifs promote desiccation tolerance in vivo.

BMC biology

Hibshman JD,Goldstein B

MA1-80189 was used in Western Blotting to provide insights into the endogenous functions and expression dynamics of an LEA protein in a multicellular animal.

Tue Dec 14 00:00:00 EST 2021

Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.

The Journal of cell biology

Nsamba ET,Bera A,Costanzo M,Boone C,Gupta ML

MA1-80189 was used in Western Blotting to provide novel mechanistic insights into how tubulin isotypes allow highly conserved microtubules to function in diverse cellular processes.

Mon Dec 06 00:00:00 EST 2021

Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.

Nucleic acids research

Kaczmarek Michaels K,Mohd Mostafa S,Ruiz Capella J,Moore CL

MA1-80189 was used in Western Blotting to our study suggests that the methyltransferases Set1 and Set2 regulate APA induced by nutritional stress, affect the RNAP II C-terminal domain phosphorylation at Ser2, and control recruitment of the 3' end processing machinery to the vicinity of pA sites.

Thu Jun 04 00:00:00 EDT 2020

Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.

Nucleic acids research

Kaczmarek Michaels K,Mohd Mostafa S,Ruiz Capella J,Moore CL

Thu Jun 04 00:00:00 EDT 2020

Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assembly.

Journal of cell science

McElmurry K,Stone JE,Ma D,Lamoureux P,Zhang Y,Steidemann M,Fix L,Huang F,Miller KE,Suter DM

MA1-80189 was used in Immunocytochemistry to suggest that microtubule (MT) assembly is required for dynein-driven MT translocation and neurite outgrowth.

Fri Apr 24 00:00:00 EDT 2020

Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H.

Cellular signalling

Tachie-Menson T,Gázquez-Gutiérrez A,Fulcher LJ,Macartney TJ,Wood NT,Varghese J,Gourlay R,Soares RF,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Sat Aug 01 00:00:00 EDT 2020

Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H.

Cellular signalling

Tachie-Menson T,Gázquez-Gutiérrez A,Fulcher LJ,Macartney TJ,Wood NT,Varghese J,Gourlay R,Soares RF,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Sat Aug 01 00:00:00 EDT 2020

A Noncanonical DNA Damage Checkpoint Response in a Major Fungal Pathogen.

mBio

Shor E,Garcia-Rubio R,DeGregorio L,Perlin DS

MA1-80189 was used in Western Blotting to show that, unlike S. cerevisiae, C. glabrata cells exposed to DNA damage did not induce C. glabrata Rad53 (CgRad53) phosphorylation.

Tue Dec 15 00:00:00 EST 2020

A Noncanonical DNA Damage Checkpoint Response in a Major Fungal Pathogen.

mBio

Shor E,Garcia-Rubio R,DeGregorio L,Perlin DS

MA1-80189 was used in Western Blotting to show that, unlike S. cerevisiae, C. glabrata cells exposed to DNA damage did not induce C. glabrata Rad53 (CgRad53) phosphorylation.

Tue Dec 15 00:00:00 EST 2020

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

MA1-80189 was used in Immunocytochemistry to imply that endogenous RAS proteins can be targeted for proteolysis, supporting the idea of an alternative therapeutic approach to these undruggable targets.

Thu Sep 17 00:00:00 EDT 2020

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Thu Sep 17 00:00:00 EDT 2020

Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System.

Cell chemical biology

Simpson LM,Macartney TJ,Nardin A,Fulcher LJ,Röth S,Testa A,Maniaci C,Ciulli A,Ganley IG,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System.

Cell chemical biology

Simpson LM,Macartney TJ,Nardin A,Fulcher LJ,Röth S,Testa A,Maniaci C,Ciulli A,Ganley IG,Sapkota GP

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo.

Cell

Nil Z,Hervás R,Gerbich T,Leal P,Yu Z,Saraf A,Sardiu M,Lange JJ,Yi K,Unruh J,Slaughter B,Si K

MA1-80189 was used in Western Blotting to identification of a prion-like protein, Herzog, through a multimodal screen in Drosophila melanogaster, herzog functions as a membrane-associated phosphatase and controls embryonic patterning.

Thu Sep 05 00:00:00 EDT 2019

Live-cell phenotypic-biomarker microfluidic assay for the risk stratification of cancer patients via machine learning.

Nature biomedical engineering

Manak MS,Varsanik JS,Hogan BJ,Whitfield MJ,Su WR,Joshi N,Steinke N,Min A,Berger D,Saphirstein RJ,Dixit G,Meyyappan T,Chu HM,Knopf KB,Albala DM,Sant GR,Chander AC

MA1-80189 was used in Immunocytochemistry-immunoflourescence to develop the live-primary-cell phenotypic-biomarker assay with single-cell resolution and its validation with prostate cancer and breast cancer tissue samples for prediction of post-surgical adverse pathology.

Mon Oct 01 00:00:00 EDT 2018

PAWS1 controls Wnt signalling through association with casein kinase 1α.

EMBO reports

Bozatzi P,Dingwell KS,Wu KZ,Cooper F,Cummins TD,Hutchinson LD,Vogt J,Wood NT,Macartney TJ,Varghese J,Gourlay R,Campbell DG,Smith JC,Sapkota GP

MA1-80189 was used in Western Blot to show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication.

Sun Apr 01 00:00:00 EDT 2018

Functional role of Tia1/Pub1 and Sup35 prion domains: directing protein synthesis machinery to the tubulin cytoskeleton.

Molecular cell

Li X,Rayman JB,Kandel ER,Derkatch IL

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-80189) in Immunoprecipitation

Thu Jul 17 00:00:00 EDT 2014

A coordinated interdependent protein circuitry stabilizes the kinetochore ensemble to protect CENP-A in the human pathogenic yeast Candida albicans.

PLoS genetics

Thakur J,Sanyal K

MA1-80189 was used in immunocytochemistry to study kinetochore assembly and disassembly in Candida albicans

Wed Sep 26 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; TUB1; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBB2B; YML085C

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UniProt ID: (Baker's yeast) P09733, (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) Q5XJF8, (Mouse) P05213, (Mouse) P05216, (Mouse) P68368, (Mouse) Q3UX10, (Mouse) Q9CWF2

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Entrez Gene ID: (Baker's yeast) 854889, (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733, (Mouse) 22142, (Mouse) 22143, (Mouse) 22146, (Mouse) 22145, (Mouse) 238463, (Mouse) 73710

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Function(s)

nucleotide binding molecular_function structural constituent of cytoskeleton GTP binding GTPase activity structural molecule activity protein binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding enzyme binding tubulin

Process(es)

mitotic sister chromatid segregation microtubule cytoskeleton organization mitotic cell cycle nuclear division microtubule-based process nuclear migration along microtubule homologous chromosome segregation nuclear migration by microtubule mediated pushing forces G2/M transition of mitotic cell cycle cytoskeleton organization cytoskeleton-dependent intracellular transport cell division cellular response to interleukin-4 biological_process platelet degranulation neuron migration protein polymerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Tubulin Monoclonal Antibody (YOL1/34)

alpha Tubulin Antibody (MA1-80189) in ICC/IF

Product Details

MA1-80189

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Radioimmune Assays (RIA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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