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bamD Antibody (PA5-144407) in WB
Western blot analysis of bamD in recombinant protein using a dilution of 1:2,000. Samples were incubated with bamD polyclonal antibody (Product # PA5-144407). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 29.9 kDa. Observed band size: 35 kDa.
Product Details
PA5-144407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Escherichia coli (strain K12) BAMD protein (20-245aa)
Conjugate
Form
Liquid
Concentration
3.55 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3091624
Target Information
Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamA, the core component of the assembly machinery. Probably involved in transient protein interactions. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: yfiO
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