Invitrogen

beta-1 Adrenergic Receptor Polyclonal Antibody

6 Published Figures

21 References

View all (14) beta-1 Adrenergic Receptor antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (6)

beta-1 Adrenergic Receptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

beta-1 Adrenergic Receptor Antibody (PA1-049) in ICC/IF

Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 (Product # PA1-049), at a dilution of 1:100 for at ... View More

beta-1 Adrenergic Receptor Antibody in Immunohistochemistry (IHC)

beta-1 Adrenergic Receptor Antibody in Western Blot (WB)

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Product Details

PA1-049

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

Assay-dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Cat, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2289444

Product Specific Information

PA1-049 detects beta-1 adrenergic receptor (B1AR) from , human, mouse, non-human primate, and rat tissues.

PA1-049 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~50.5 kDa band representing B1AR in mouse kidney membrane preparation. Immunofluorescence staining of B1AR in mouse kidney distal tubule with PA1-049 yields a pattern consistent with plasma membrane staining.

The PA1-049 immunogen is a synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. This sequence is completely conserved between mouse and rat B1AR.

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CaMKII and reactive oxygen species contribute to early reperfusion arrhythmias, but oxidation of CaMKIIδ at methionines 281/282 is not a determining factor.

Journal of molecular and cellular cardiology

Haugsten Hansen M,Sadredini M,Hasic A,Anderson ME,Sjaastad I,Korseberg Stokke M

PA1-049 was used in Western Blotting to investigate the role of oxidized-CaMKIIδ (Ox-CaMKIIδ) in early ischemia-reperfusion arrhythmias (IRA).

Wed Feb 01 00:00:00 EST 2023

Basolateral amygdala activation enhances object recognition memory by inhibiting anterior insular cortex activity.

Proceedings of the National Academy of Sciences of the United States of America

Chen YF,Song Q,Colucci P,Maltese F,Siller-Pérez C,Prins K,McGaugh JL,Hermans EJ,Campolongo P,Nadif Kasri N,Roozendaal B

PA1-049 was used in Western Blotting to investigate, in male Sprague-Dawley rats, whether the basolateral amygdala influence on recognition memory is associated with an increase or suppression of anterior insular cortex activity during the postlearning consolidation period.

Tue May 31 00:00:00 EDT 2022

Basolateral amygdala activation enhances object recognition memory by inhibiting anterior insular cortex activity.

Proceedings of the National Academy of Sciences of the United States of America

Chen YF,Song Q,Colucci P,Maltese F,Siller-Pérez C,Prins K,McGaugh JL,Hermans EJ,Campolongo P,Nadif Kasri N,Roozendaal B

Tue May 31 00:00:00 EDT 2022

Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity.

Nutrients

Osorio-Conles Ó,Olbeyra R,Moizé V,Ibarzabal A,Giró O,Viaplana J,Jiménez A,Vidal J,de Hollanda A

PA1-049 was used in Immunohistochemistry to support a protective effect of a Mediterranean diet supplemented with almonds on obesity-related WAT dysfunction.

Fri Jun 24 00:00:00 EDT 2022

Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity.

Nutrients

Osorio-Conles Ó,Olbeyra R,Moizé V,Ibarzabal A,Giró O,Viaplana J,Jiménez A,Vidal J,de Hollanda A

PA1-049 was used in Immunohistochemistry to support a protective effect of a Mediterranean diet supplemented with almonds on obesity-related WAT dysfunction.

Fri Jun 24 00:00:00 EDT 2022

Norepinephrine activates β₁ -adrenergic receptors at the inner nuclear membrane in astrocytes.

Glia

Benton KC,Wheeler DS,Kurtoglu B,Ansari MBZ,Cibich DP,Gonzalez DA,Herbst MR,Khursheed S,Knorr RC,Lobner D,Maglasang JG,Rohr KE,Taylor A,Twining RC,Witt PJ,Gasser PJ

PA1-049 was used in Western Blotting to reveal a novel mechanism by which norepinephrine may directly influence nuclear processes.

Thu Sep 01 00:00:00 EDT 2022

Adenylyl cyclase isoform 1 contributes to sinoatrial node automaticity via functional microdomains.

JCI insight

Ren L,Thai PN,Gopireddy RR,Timofeyev V,Ledford HA,Woltz RL,Park S,Puglisi JL,Moreno CM,Santana LF,Conti AC,Kotlikoff MI,Xiang YK,Yarov-Yarovoy V,Zaccolo M,Zhang XD,Yamoah EN,Navedo MF,Chiamvimonvat N

PA1-049 was used in Western Blotting to provide insights into the critical roles of the Ca2+-activated isoform of AC in sustaining SAN automaticity that is distinct from contractile cardiomyocytes.

Tue Nov 22 00:00:00 EST 2022

Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Lee HJ,Jin BY,Park MR,Seo KS,Jeong YT,Choi SH,Kim DH

PA1-049 was used in Immunohistochemistry to identify the role of each β-AR subtype in the regulation of ATBF in mice.

Thu Jul 01 00:00:00 EDT 2021

Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Lee HJ,Jin BY,Park MR,Seo KS,Jeong YT,Choi SH,Kim DH

PA1-049 was used in Immunohistochemistry to identify the role of each β-AR subtype in the regulation of ATBF in mice.

Thu Jul 01 00:00:00 EDT 2021

Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.

Nature communications

Funato Y,Yamazaki D,Okuzaki D,Yamamoto N,Miki H

PA1-049 was used in Western Blot to indicate crucial roles of TRPM6 in the circadian regulation of blood pressure.

Thu Jun 17 00:00:00 EDT 2021

Exercise Training Stabilizes RyR2-Dependent Ca²⁺ Release in Post-infarction Heart Failure.

Frontiers in cardiovascular medicine

Danielsen TK,Sadredini M,Manotheepan R,Aronsen JM,Frisk M,Hansen MH,Andressen KW,Hougen K,Levy FO,Louch WE,Sejersted OM,Sjaastad I,Stokke MK

PA1-049 was used in Western Blotting to show that these effects of ET can be gained without major alterations in SR Ca2+ regulatory proteins and indicate that future studies should include upstream parts of the sympathetic signaling pathway.

Fri Feb 12 00:00:00 EST 2021

Cardiomyocyte slowly activating delayed rectifier potassium channel: regulation by exercise and β-adrenergic signaling.

Journal of applied physiology (Bethesda, Md. : 1985)

Wang X,Fitts RH

PA1-049 was used in Western Blotting to elucidate the mechanisms by which exercise training alters β-adrenergic regulation and to determine the involvement of delayed rectifier potassium channels (IKr and IKs) in the response.

Fri May 01 00:00:00 EDT 2020

Aldosterone Jeopardizes Myocardial Insulin and β-Adrenergic Receptor Signaling via G Protein-Coupled Receptor Kinase 2.

Frontiers in pharmacology

Cannavo A,Marzano F,Elia A,Liccardo D,Bencivenga L,Gambino G,Perna C,Rapacciuolo A,Cittadini A,Ferrara N,Paolocci N,Koch WJ,Rengo G

Published figure using beta-1 Adrenergic Receptor polyclonal antibody (Product # PA1-049) in Western Blot

Tue Sep 29 00:00:00 EDT 2020

Regulation of cardiac myocyte cohesion and gap junctions via desmosomal adhesion.

Acta physiologica (Oxford, England)

Schinner C,Erber BM,Yeruva S,Waschke J

PA1-049 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to support the identification of new targets to treat arrhythmogenic cardiomyopathy.

Sat Jun 01 00:00:00 EDT 2019

Atrial arrhythmias and autonomic dysfunction in rats exposed to chronic intermittent hypoxia.

American journal of physiology. Heart and circulatory physiology

Bober SL,Ciriello J,Jones DL

PA1-049 was used in Western Blotting to study the effect of chronic intermittent hypoxia on atrial electrophysiology in atrial fibrillation.

Fri Jun 01 00:00:00 EDT 2018

Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line.

International journal of molecular sciences

Tyurin-Kuzmin PA,Chechekhin VI,Ivanova AM,Dyikanov DT,Sysoeva VY,Kalinina NI,Tkachuk VA

PA1-049 was used in Immunocytochemistry-immunoflourescence to study the usage of immortalised cells for multipotent stem/stromal cell-based regenerative medicine.

Thu Nov 22 00:00:00 EST 2018

Upregulation of β1-adrenoceptors is involved in the formation of gastric dysmotility in the 6-hydroxydopamine rat model of Parkinson's disease.

Translational research : the journal of laboratory and clinical medicine

Song J,Zheng L,Zhang X,Feng X,Fan R,Sun L,Hong F,Zhang Y,Zhu J

PA1-049 was used in immunohistochemistry and western blot to study gastric paresis in a rat model of Parkinson's disease and the the role played by increased levels of beta1-adrenoceptors

Tue Jul 01 00:00:00 EDT 2014

Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways.

Age (Dordrecht, Netherlands)

Rudolf R,Bogomolovas J,Strack S,Choi KR,Khan MM,Wagner A,Brohm K,Hanashima A,Gasch A,Labeit D,Labeit S

Published figure using beta-1 Adrenergic Receptor polyclonal antibody (Product # PA1-049) in Western Blot

Tue Oct 01 00:00:00 EDT 2013

Heterogeneous ventricular sympathetic innervation, altered beta-adrenergic receptor expression, and rhythm instability in mice lacking the p75 neurotrophin receptor.

American journal of physiology. Heart and circulatory physiology

Lorentz CU,Alston EN,Belcik T,Lindner JR,Giraud GD,Habecker BA

PA1-049 was used in western blot to study the role of p75 neurotrophin receptor in innervation and rhythm stability in mice

Tue Jun 01 00:00:00 EDT 2010

Norepinephrine transporter is involved in down-regulation of beta1-adrenergic receptors caused by adjuvant arthritis.

Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Clements JD,Jamali F

PA1-049 was used in western blot to investigate the effects of inflammation on beta1-adrenergic receptors and norepinephrine transporter (NET) density

Fri Apr 02 00:00:00 EDT 2010

Adrenergic receptor agonists prevent bile duct injury induced by adrenergic denervation by increased cAMP levels and activation of Akt.

American journal of physiology. Gastrointestinal and liver physiology

Glaser S,Alvaro D,Francis H,Ueno Y,Marucci L,Benedetti A,De Morrow S,Marzioni M,Mancino MG,Phinizy JL,Reichenbach R,Fava G,Summers R,Venter J,Alpini G

PA1-049 was used in immunocytochemistry to study how cholangiocyte proliferative and secretory responses to bile duct ligation are influenced by adrenergic innervation

Sat Apr 01 00:00:00 EST 2006

Infusion of the beta-adrenergic blocker esmolol attenuates myocardial dysfunction in septic rats.

Critical care medicine

Suzuki T,Morisaki H,Serita R,Yamamoto M,Kotake Y,Ishizaka A,Takeda J

PA1-049 was used in immunohistochemistry to investigate the effect of the beta-adrenergic blocker esmolol on myocardial dysfunction in septic rats

Sat Oct 01 00:00:00 EDT 2005

Cardioprotective effects of exercise training on myofilament calcium activation in ovariectomized rats.

Journal of applied physiology (Bethesda, Md. : 1985)

Bupha-Intr T,Wattanapermpool J

PA1-049 was used in western blot to study whether the cardioprotective effects of exercise training affects calcium activation in cardiac myofilament in ovariectomized rats

Sat May 01 00:00:00 EDT 2004

Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.

Neuroscience research

Nakadate K,Imamura K,Watanabe Y

PA1-049 was used in immunohistochemistry to study the regulation of adrenergic receptors by sensory afferents in kitten brains

Fri Jun 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: adrenergic receptor, beta 1; adrenergic, beta-1-, receptor; beta 1-adrenergic receptor beta 1-AR; beta 1-AR; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; cardiac beta adrenergic receptor

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Gene Aliases: Adrb-1; ADRB1; ADRB1R; B1AR; beta-AR; BETA1AR; RATB1AR; RHR

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UniProt ID: (Human) P08588, (Mouse) P34971, (Rat) P18090

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Entrez Gene ID: (Human) 153, (Mouse) 11554, (Rat) 24925

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Function(s)

beta-adrenergic receptor activity beta1-adrenergic receptor activity receptor signaling protein activity Ras guanyl-nucleotide exchange factor activity protein binding PDZ domain binding alpha-2A adrenergic receptor binding protein heterodimerization activity epinephrine binding norepinephrine binding signal transducer activity G-protein coupled receptor activity adrenergic receptor activity drug binding dopamine binding G-protein coupled receptor

Process(es)

Performance Guarantee

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beta-1 Adrenergic Receptor Polyclonal Antibody

beta-1 Adrenergic Receptor Antibody (PA1-049) in ICC/IF

Product Details

PA1-049

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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