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Invitrogen
beta-3 Tubulin Monoclonal Antibody (2G10-TB3), eFluor™ 660, eBioscience™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
beta-3 Tubulin Antibody (50-4510-82) in ICC/IF
Knockout of beta-3 Tubulin (TUBB3) was achieved by CRISPR-Cas9 genome editing. Immunofluorescence analysis was performed on U-87 MG Cas9 cells (panels a,c) and U-87 MG beta-3 Tubulin KO cells (panel b,d). Cells were fixed, permeabilized, and labelled with beta-3 Tubulin Monoclonal Antibody, eFluor 660 (Product # 50-4510-82) at a concentration of 820310 µg/mL. Nuclei (blue) were stained using ProLong™ Diamon... View More
Product Details
50-4510-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2G10-TB3
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574213
Product Specific Information
Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network. Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals. Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone. Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells. Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III.
Applications Reported: This 2G10-TB3 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This 2G10-TB3 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4
Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4
UniProt ID: (Bovine) Q2T9S0, (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4
Entrez Gene ID: (Bovine) 768070, (Human) 10381, (Mouse) 22152, (Rat) 246118
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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