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Invitrogen
beta Actin Recombinant Rabbit Monoclonal Antibody (4C4)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
beta Actin Antibody (MA5-42946) in WB
Western blot analysis of beta Actin in extracts of various cell lines. Samples were incubated with beta Actin Monoclonal antibody (Product # MA5-42946) using a dilution of 1:50,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
MA5-42946
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4C4
Immunogen
Recombinant protein of human beta-Actin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2912087
Product Specific Information
Positive test controls include: 293T, HeLa, Mouse brain, Mouse testis, Mouse lung, Rat brain, Rat testis, Rat lung. The target is usually found in the following locations: Cytoplasm, cytoskeleton.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin, beta; Actin, cytoplasmic 1; B-ACTZF; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin; PS1TP5-binding protein 1
Gene Aliases: ACTB; actba; actin; Actx; B-ACTZF; Bact; bactin1; bactzf; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05
UniProt ID: (Human) P60709, (Pig) Q6QAQ1, (Chicken) P60706, (Rat) P60711, (Mouse) P60710, (Zebrafish) Q7ZVI7
Entrez Gene ID: (Human) 60, (Pig) 414396, (Chicken) 396526, (Rat) 81822, (Mouse) 11461, (Zebrafish) 57934
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
nucleotide binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
postsynaptic actin cytoskeleton organization
axonogenesis
cell motility
retina development in camera-type eye
cellular response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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