Invitrogen

beta Actin Monoclonal Antibody (8H10D10)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

11 References

View all (92) beta Actin antibodies

Datasheet

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Questions & Answers

Datasheet

Protocols

Questions & Answers

beta Actin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

beta Actin Antibody (MA5-15452) in ICC/IF

Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta-Actin monoclonal antibody (Product # MA5-15452) (Red). Blue: DRAQ5 fluorescent DNA dye. Red: the secondary Ab is Cy3-Goat anti mouse IgG

beta Actin Antibody in Western Blot (WB)

beta Actin Antibody in Flow Cytometry (Flow)

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Product Details

MA5-15452

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

8H10D10

Immunogen

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11001306

Product Specific Information

MA5-15452 targets beta-Actin in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Hamster, Human, mouse, Non-human primate, and Rat samples.

The MA5-15452 immunogen is synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

MA5-15452 detects beta-Actin which has a predicted molecular weight of approximately 42kDa.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Two point mutations in protocadherin-1 disrupt hantavirus recognition and afford protection against lethal infection.

Nature communications

Slough MM,Li R,Herbert AS,Lasso G,Kuehne AI,Monticelli SR,Bakken RR,Liu Y,Ghosh A,Moreau AM,Zeng X,Rey FA,Guardado-Calvo P,Almo SC,Dye JM,Jangra RK,Wang Z,Chandran K

MA5-15452 was used in Western Blotting to delineate the binding surface of the hantavirus glycoprotein complex on PCDH1's first extracellular cadherin repeat domain.

Mon Jul 24 00:00:00 EDT 2023

Parecoxib inhibits esophageal squamous cell carcinoma progression via the PDK1-AKT pathway.

Cellular & molecular biology letters

Huang HM,Huang XY,Wu SP,Chen CK,He XH,Zhang YF

MA5-15452 was used in Western Blot to conclude that parecoxib inhibits ESCC progression, including cell cycle, proliferation, invasion, and migration, via the PDK1-AKT signaling pathway.

Sat Mar 19 00:00:00 EDT 2022

Pituitary adenylate cyclase-activating polypeptide receptor activation in the hypothalamus recruits unique signaling pathways involved in energy homeostasis.

American journal of physiology. Endocrinology and metabolism

Maunze B,Bruckner KW,Desai NN,Chen C,Chen F,Baker D,Choi S

MA5-15452 was used in Western Blotting to reveal that endogenous PAC1Rs uniquely engage PKA, PKC, and receptor trafficking to mediate PACAP's pleiotropic effects in VMN control of feeding and metabolism.

Tue Mar 01 00:00:00 EST 2022

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using beta Actin monoclonal antibody (Product # MA5-15452) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Immunomodulatory effects of Radix isatidis polysaccharides in vitro and in vivo.

Experimental and therapeutic medicine

Tao W,Fu T,He ZJ,Zhou HP,Hong Y

MA5-15452 was used in Western Blot to suggest that RIP could be potentially used as a novel immunomodulator.

Wed Dec 01 00:00:00 EST 2021

The hepcidin regulator erythroferrone is a new member of the erythropoiesis-iron-bone circuitry.

eLife

Castro-Mollo M,Gera S,Ruiz-Martinez M,Feola M,Gumerova A,Planoutene M,Clementelli C,Sangkhae V,Casu C,Kim SM,Ostland V,Han H,Nemeth E,Fleming R,Rivella S,Lizneva D,Yuen T,Zaidi M,Ginzburg Y

MA5-15452 was used in Western Blot to show that ERFE exerts an osteoprotective effect by modulating BMP signaling in osteoblasts, decreasing RANKL production to limit osteoclastogenesis, and prevents excessive bone loss during expanded erythropoiesis in β-thalassemia.

Tue May 18 00:00:00 EDT 2021

The hepcidin regulator erythroferrone is a new member of the erythropoiesis-iron-bone circuitry.

eLife

Castro-Mollo M,Gera S,Ruiz-Martinez M,Feola M,Gumerova A,Planoutene M,Clementelli C,Sangkhae V,Casu C,Kim SM,Ostland V,Han H,Nemeth E,Fleming R,Rivella S,Lizneva D,Yuen T,Zaidi M,Ginzburg Y

Tue May 18 00:00:00 EDT 2021

Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma.

Cancer cell

Dheilly E,Battistello E,Katanayeva N,Sungalee S,Michaux J,Duns G,Wehrle S,Sordet-Dessimoz J,Mina M,Racle J,Farinha P,Coukos G,Gfeller D,Mottok A,Kridel R,Correia BE,Steidl C,Bassani-Sternberg M,Ciriello G,Zoete V,Oricchio E

MA5-15452 was used in Western Blotting to find that follicular lymphoma patients harbor a recurrent hotspot mutation targeting tyrosine 132 (Y132D) in cathepsin S (CTSS) that enhances protein activity.

Mon May 11 00:00:00 EDT 2020

Chromatin Remodeling in Response to BRCA2-Crisis.

Cell reports

Gruber JJ,Chen J,Geller B,Jäger N,Lipchik AM,Wang G,Kurian AW,Ford JM,Snyder MP

Published figure using beta Actin monoclonal antibody (Product # MA5-15452) in Western Blot

Tue Aug 20 00:00:00 EDT 2019

Prohibitin is a prognostic marker and therapeutic target to block chemotherapy resistance in Wilms' tumor.

JCI insight

Ortiz MV,Ahmed S,Burns M,Henssen AG,Hollmann TJ,MacArthur I,Gunasekera S,Gaewsky L,Bradwin G,Ryan J,Letai A,He Y,Naranjo A,Chi YY,LaQuaglia M,Heaton T,Cifani P,Dome JS,Gadd S,Perlman E,Mullen E,Steen H,Kentsis A

MA5-15452 was used in Western Blot to identify urine tumor markers associated with Wilms' tumor relapse.

Thu Aug 08 00:00:00 EDT 2019

Influence of Matrices on 3D-Cultured Prostate Cancer Cells' Drug Response and Expression of Drug-Action Associated Proteins.

PloS one

Edmondson R,Adcock AF,Yang L

MA5-15452 was used in western blot to characterize 3D-cultured prostate cancer cells' drug response and expression of drug-action associated proteins and the influence of matrices

Mon Jul 24 00:00:00 EDT 2017

An Antibody-Drug Conjugate Directed against Lymphocyte Antigen 6 Complex, Locus E (LY6E) Provides Robust Tumor Killing in a Wide Range of Solid Tumor Malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Asundi J,Crocker L,Tremayne J,Chang P,Sakanaka C,Tanguay J,Spencer S,Chalasani S,Luis E,Gascoigne K,Desai R,Raja R,Friedman BA,Haverty PM,Polakis P,Firestein R

MA5-15452 was used in immunohistochemistry - paraffin section to discuss the use of antibody-drug conjugates to deliver cytotoxic agents to tumors

Wed Jul 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; PS1TP5-binding protein 1

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Gene Aliases: ACTB; Actx; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

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UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975

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Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta Actin Monoclonal Antibody (8H10D10)

beta Actin Antibody (MA5-15452) in ICC/IF

Product Details

MA5-15452

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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