Invitrogen

beta Actin Recombinant Rabbit Monoclonal Antibody (JF53-10), HRP

beta Actin Antibody in Western Blot (WB)

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beta Actin Antibody (MA5-32540) in WB

Western blot analysis of beta Actin in different lysates using a Monoclonal antibody (Product #MA5-32540) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: NIH/3T3, Lane 4: zebrafish.

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Product Details

MA5-32540

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

View 4 publications 4 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Plant, Rat, Zebrafish

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF53-10

Immunogen

Recombinant full length protein of Human beta Actin aa 1-375

View immunogen

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809817

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Natural Steroidal Lactone Induces G1/S Phase Cell Cycle Arrest and Intrinsic Apoptotic Pathway by Up-Regulating Tumor Suppressive miRNA in Triple-Negative Breast Cancer Cells.

Metabolites

Shuaib M,Prajapati KS,Gupta S,Kumar S

MA5-32540 was used in Western Blotting to indicate that the Withaferin A and miRNA mimic co-treatment strategy may be utilized as a newer therapeutic strategy to treat triple-negative breast cancer.

Sat Dec 24 00:00:00 EST 2022

Dissecting the genetic and microenvironmental factors of gastric tumorigenesis in mice.

Cell reports

Lu Z,Zhong A,Liu H,Zhang M,Chen X,Pan X,Wang M,Deng X,Gao L,Zhao L,Wang J,Yang Y,Zhang Q,Wu B,Zheng J,Wang Y,Song X,Liu K,Zhang W,Chen X,Yang K,Chen X,Zhao Y,Zhao C,Wang Y,Chen L,Zhou Z,Hu J,Liu Y,Chen C

MA5-32540 was used in Western Blotting to propose a strategy to model GC and dissect the genetic and microenvironmental factors driving the full-range gastric tumorigenesis.

Tue Oct 18 00:00:00 EDT 2022

Endothelial Heme Dynamics Drive Cancer Cell Metabolism by Shaping the Tumor Microenvironment.

Biomedicines

Petrillo S,De Giorgio F,Kopecka J,Genova T,Fiorito V,Allocco AL,Bertino F,Chiabrando D,Mussano F,Altruda F,Munaron L,Riganti C,Tolosano E

MA5-32540 was used in Western Blot to show that interfering with endothelial heme metabolism by targeting the cell membrane heme exporter Feline Leukemia Virus subgroup C Receptor 1a (FLVCR1a) in tumor-associated endothelial cells resulted in enhanced fatty acid oxidation.

Thu Oct 28 00:00:00 EDT 2021

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

MA5-32540 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

MA5-32540 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic beta-actin; PS1TP5-binding protein 1

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Gene Aliases: ACTB; actba; Actx; B-ACTZF; bact; bactin1; bactzf; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05

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UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975, (Zebrafish) Q7ZVI7

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Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477, (Zebrafish) 57934

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding nucleotide binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta Actin Recombinant Rabbit Monoclonal Antibody (JF53-10), HRP

beta Actin Antibody (MA5-32540) in WB

Product Details

MA5-32540

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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