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beta-NGF Antibody (500-P85BT-25UG) in ELISA
Sandwich ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of beta-NGF Polyclonal Antibody, Biotin (Product # 500-P85BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech beta-NGF Polyclonal Antibody (Product # 500-P85-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human beta-NGF.
Product Details
500-P85BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 27.0 kDa Recombinant Human beta-NGF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930813
Product Specific Information
AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human beta-NGF. Anti-Human beta-NGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human beta-NGF (500-P85) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human beta-NGF.
Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P85BT-1MG will be provided as 2 x 500 µg
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B nerve growth factor; b-NGF; beta ngf; Beta-nerve growth factor; Beta-NGF; BetaNGF; bNGF; MGC161426; MGC161428; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGF b; NGF β; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
Gene Aliases: Beta-NGF; HSAN5; NGF; NGFB
UniProt ID: (Human) P01138
Entrez Gene ID: (Human) 4803
activation of MAPKK activity
activation of cysteine-type endopeptidase activity involved in apoptotic process
microtubule-based movement
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of gene expression
nerve growth factor processing
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
regulation of neuron differentiation
positive regulation of Ras protein signal transduction
neurotrophin TRK receptor signaling pathway
phosphatidylinositol-mediated signaling
neuron projection morphogenesis
positive regulation of axonogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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