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Invitrogen
beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 550
FIGURE: 1 / 2
beta Tubulin Loading Control Antibody (MA5-16308-D550) in ICC/IF
Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 550 (Product # MA5-16308-D550) at 1:100 dilut... View More
Product Details
MA5-16308-D550
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BT7R
Immunogen
KLH conjugated peptide of the N-terminus of Beta-Tubulin.
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_2537823
Product Specific Information
MA5-16308-D550 has successfully been used for immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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