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Invitrogen
c-Fos Recombinant Rabbit Monoclonal Antibody (JJ0938)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
c-Fos Antibody (MA5-32492) in ICC/IF
Immunocytochemistry analysis of c-Fos with normal HeLa/HeLa serum. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal c-Fos (Product # MA5-32492) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-32492
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ0938
Immunogen
Synthetic peptide within Human c-Fos aa 231-268
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809769
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator protein 1; c-fos oncogene; cellular oncogene c-fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ osteosarcoma oncogene; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Protein c-Fos; Proto-oncogene c-Fos; proto-oncogene cFOS; proto-oncogene protein c-fos; Transcription factor AP-1 subunit c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog
Gene Aliases: AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55
UniProt ID: (Human) P01100, (Mouse) P01101, (Rat) P12841
Entrez Gene ID: (Human) 2353, (Mouse) 14281, (Rat) 314322
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
transcription regulatory region DNA binding
protein heterodimerization activity
R-SMAD binding
DNA binding
double-stranded DNA binding
sequence-specific DNA binding
basic leucine zipper transcription factor
conditioned taste aversion
DNA methylation
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
inflammatory response
transforming growth factor beta receptor signaling pathway
nervous system development
female pregnancy
aging
response to cold
response to light stimulus
response to gravity
response to toxic substance
sleep
cellular response to extracellular stimulus
response to lipopolysaccharide
response to progesterone
cellular response to hormone stimulus
response to cytokine
cellular response to reactive oxygen species
response to immobilization stress
skeletal muscle cell differentiation
response to muscle stretch
Fc-epsilon receptor signaling pathway
response to drug
positive regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of sequence-specific DNA binding transcription factor activity
response to corticosterone
response to cAMP
SMAD protein signal transduction
cellular response to calcium ion
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
response to mechanical stimulus
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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