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Invitrogen
c-Jun Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
PA1-833
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
IgG fraction
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2129865
Product Specific Information
PA1-833 detects cJun from mouse cells.
PA1-833 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.
The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
Reconstitute in 200 µL PBS to create a stock of 1 mg/mL.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activator protein 1; AH119; AP1; cjun; immediate early; Jun A; Jun oncogene; JUND; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; c-jun; Jun; Junc
UniProt ID: (Mouse) P05627
Entrez Gene ID: (Mouse) 16476
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
GTPase activator activity
protein binding
transcription factor binding
enzyme binding
cAMP response element binding
protein homodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
R-SMAD binding
HMG box domain binding
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
microglial cell activation
release of cytochrome c from mitochondria
liver development
positive regulation of endothelial cell proliferation
outflow tract morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
SMAD protein import into nucleus
learning
positive regulation of cell proliferation
negative regulation of cell proliferation
response to radiation
cellular process
monocyte differentiation
axon regeneration
negative regulation of protein autophosphorylation
response to lipopolysaccharide
cellular response to hormone stimulus
response to cytokine
leading edge cell differentiation
response to muscle stretch
response to drug
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of DNA binding
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
negative regulation by host of viral transcription
positive regulation by host of viral transcription
positive regulation of cell differentiation
positive regulation of monocyte differentiation
positive regulation of DNA replication
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
positive regulation of smooth muscle cell proliferation
cellular response to potassium ion starvation
response to cAMP
regulation of cell cycle
membrane depolarization
SMAD protein signal transduction
cellular response to calcium ion
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
positive regulation of DNA-templated transcription, initiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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