Invitrogen

c-Jun Monoclonal Antibody (5B1)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures
5 References
View all (149) c-Jun antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-15881

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B1

Immunogen

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153582

Product Specific Information

MA5-15881 targets c-Jun in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15881 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli. .

MA5-15881 detects c-Jun which has a predicted molecular weight of approximately 43kDa.

Target Information

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

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Gene Aliases: AP-1; AP1; c-Jun; JUN; Junc

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UniProt ID: (Human) P05412, (Mouse) P05627

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Entrez Gene ID: (Human) 3725, (Mouse) 16476

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor
Process(es)
angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus Ras protein signal transduction aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process positive regulation of apoptotic process negative regulation of apoptotic process
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