Invitrogen

c-Met Polyclonal Antibody

Advanced Verification

IP-mass spectrometry

11 Published Figures

12 References

View all (88) c-Met antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

c-Met Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

c-Met Antibody (71-8000) in IHC (P)

Immunohistochemistry analysis of c-Met showing staining in the cytoplasm and membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at r... View More

c-Met Antibody in Immunohistochemistry (IHC)

Product Image Gallery

c-Met Antibody — IP-MS enrichment of MET (LFQ intensity): MET was enriched 185-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and c-MET antibody (Product # 71-8000). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

71-8000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal or the human c-Met protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533999

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of TP53 cooperates with c-MET overexpression to drive hepatocarcinogenesis.

Cell death & disease

Zhou Y,Cui G,Xu H,Chun J,Yang D,Zhang Z,Yang L,Wang J,Wan M,Calvisi DF,Lin S,Chen X,Wang H

71-8000 was used in Western Blot to demonstrate that loss of TP53 cooperates with c-MET to drive hepatocarcinogenesis in vivo.

Thu Jul 27 00:00:00 EDT 2023

Loss of TP53 cooperates with c-MET overexpression to drive hepatocarcinogenesis.

Cell death & disease

Zhou Y,Cui G,Xu H,Chun J,Yang D,Zhang Z,Yang L,Wang J,Wan M,Calvisi DF,Lin S,Chen X,Wang H

71-8000 was used in Western Blot to demonstrate that loss of TP53 cooperates with c-MET to drive hepatocarcinogenesis in vivo.

Thu Jul 27 00:00:00 EDT 2023

MET exon 14 skipping mutation is a hepatocyte growth factor (HGF)-dependent oncogenic driver in vitro and in humanised HGF knock-in mice.

Molecular oncology

Fernandes M,Hoggard B,Jamme P,Paget S,Truong MJ,Grégoire V,Vinchent A,Descarpentries C,Morabito A,Stanislovas J,Farage E,Meneboo JP,Sebda S,Bouchekioua-Bouzaghou K,Nollet M,Humez S,Perera T,Fromme P,Grumolato L,Figeac M,Copin MC,Tulasne D,Cortot AB,Kermorgant S,Kherrouche Z

71-8000 was used in ELISA, Immunohistochemistry (Paraffin), Western Blot to develop a valuable model for preclinical studies and present results that have potential clinical implication.

Wed Nov 01 00:00:00 EDT 2023

Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor.

Biochemistry and biophysics reports

Hara T,Kimura A,Miyazaki T,Tanaka H,Morimoto M,Nakai K,Soeda J

Published figure using c-Met polyclonal antibody (Product # 71-8000) in Western Blot

Sun Mar 01 00:00:00 EST 2020

Oncogene-dependent function of BRG1 in hepatocarcinogenesis.

Cell death & disease

Wang P,Song X,Cao D,Cui K,Wang J,Utpatel K,Shang R,Wang H,Che L,Evert M,Zhao K,Calvisi DF,Chen X

Published figure using c-Met polyclonal antibody (Product # 71-8000) in Western Blot

Tue Feb 04 00:00:00 EST 2020

Absent and abundant MET immunoreactivity is associated with poor prognosis of patients with oral and oropharyngeal squamous cell carcinoma.

Oncotarget

De Herdt MJ,Willems SM,van der Steen B,Noorlag R,Verhoef EI,van Leenders GJ,van Es RJ,KoljenoviÄ S,Baatenburg de Jong RJ,Looijenga LH

71-8000 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to learn the poor prognosis marker of patients with oral and oropharyngeal squamous cell carcinoma due to the absence or abundance of MET immunoreactivity

Tue Mar 15 00:00:00 EDT 2016

Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms.

International journal of cancer

Matte I,Lane D,Laplante C,Garde-Granger P,Rancourt C,Piché A

71-8000 was used in western blot to suggest that HGF and EGFR signaling mediate ovarian cancer ascites-mediated migration of human peritoneal mesothelial cells

Wed Jul 15 00:00:00 EDT 2015

p38 MAPK signaling underlies a cell-autonomous loss of stem cell self-renewal in skeletal muscle of aged mice.

Nature medicine

Bernet JD,Doles JD,Hall JK,Kelly Tanaka K,Carter TA,Olwin BB

71-8000 was used in immunocytochemistry to study the age-associated deregulation of the satellite cell homeostatic network to identify therapeutic targets.

Sat Mar 01 00:00:00 EST 2014

Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry.

The Journal of physiology

McKay BR,Toth KG,Tarnopolsky MA,Parise G

71-8000 was used in immunohistochemistry to develop a flow cytometric assay to count muscle satellite cell

Wed Sep 01 00:00:00 EDT 2010

Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.

Investigative ophthalmology & visual science

Saghizadeh M,Kramerov AA,Yu FS,Castro MG,Ljubimov AV

Thu Apr 01 00:00:00 EDT 2010

Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.

Investigative ophthalmology & visual science

Saghizadeh M,Kramerov AA,Yu FS,Castro MG,Ljubimov AV

Thu Apr 01 00:00:00 EDT 2010

Acquisition of biologically relevant gene expression data by Affymetrix microarray analysis of archival formalin-fixed paraffin-embedded tumours.

British journal of cancer

Linton KM,Hey Y,Saunders E,Jeziorska M,Denton J,Wilson CL,Swindell R,Dibben S,Miller CJ,Pepper SD,Radford JA,Freemont AJ

71-8000 was used in immunohistochemistry - paraffin section to examine archival formalin-fixed paraffin-embedded tissue samples from primary extremity soft tissue sarcomas

Tue Apr 22 00:00:00 EDT 2008

c-MET expression level in primary colon cancer: a predictor of tumor invasion and lymph node metastases.

Clinical cancer research : an official journal of the American Association for Cancer Research

Takeuchi H,Bilchik A,Saha S,Turner R,Wiese D,Tanaka M,Kuo C,Wang HJ,Hoon DS

Tue Apr 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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Gene Aliases: AUTS9; c-Met; DFNB97; HGFR; MET; RCCP2

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UniProt ID: (Human) P08581

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Entrez Gene ID: (Human) 4233

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Function(s)

protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding ATP binding protein phosphatase binding

Process(es)

endothelial cell morphogenesis signal transduction cell surface receptor signaling pathway cell proliferation negative regulation of autophagy peptidyl-tyrosine phosphorylation positive regulation of transcription from RNA polymerase II promoter hepatocyte growth factor receptor signaling pathway branching morphogenesis of an epithelial tube positive chemotaxis semaphorin-plexin signaling pathway negative regulation of hydrogen peroxide-mediated programmed cell death positive regulation of endothelial cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Met Polyclonal Antibody

c-Met Antibody (71-8000) in IHC (P)

Product Details

71-8000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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