Invitrogen

c-Myc Monoclonal Antibody (9E10.3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

51 References

View all (123) c-Myc antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

c-Myc Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

c-Myc Antibody (MA5-12080) in ICC/IF

Immunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # MA5-12080) at 1:100 dilution and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Mouse IgG (H+L) Highl... View More

c-Myc Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

c-Myc Antibody — Antibody specificity was demonstrated by detection of different targets fused to Myc tag in transiently transfected lysates tested. Relative detection of Myc tag was observed across different proteins fused with Myc tag in Fig1a. His-H3-Myc (Lane 3) and Fig1b. Myc-p65-V5 (Lane 3-6), using Anti-Myc Tag Polyclonal Antibody (Product # MA5-12080) in Western Blot. This product has been shown to detect Myc Tag at both N- and C- termini of a fusion protein. {RE}

Product Details

MA5-12080

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:200

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Immunomicroscopy (IM)

Assay-dependent

Miscellaneous PubMed (Misc)

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Tag, Xenopus, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

9E10.3

Immunogen

A synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11009013

Product Specific Information

MA5-12080 detects-myc in Human samples Does not react with mouse, rat and chicken. Others not tested..

MA5-12080 targets c-myc in FACS, IF, IHC (P), IM, IP, and WB applications.

The MA5-12080 immunogen is a synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

International journal of oncology

Wang R,Peng C,Song J,Hua Y,Wu Q,Deng L,Cao Y,Zhang J,Zhang L,Wu L,Hou L

MA5-12080 was used in Immunocytochemistry to study the effect of regulator of ribosome synthesis 1 (RRS1) on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis.

Tue Mar 01 00:00:00 EST 2022

Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis.

American journal of human genetics

Macia MS,Halbritter J,Delous M,Bredrup C,Gutter A,Filhol E,Mellgren AEC,Leh S,Bizet A,Braun DA,Gee HY,Silbermann F,Henry C,Krug P,Bole-Feysot C,Nitschké P,Joly D,Nicoud P,Paget A,Haugland H,Brackmann D,Ahmet N,Sandford R,Cengiz N,Knappskog PM,Boman H,Linghu B,Yang F,Oakeley EJ,Saint Mézard P,Sailer AW,Johansson S,Rødahl E,Saunier S,Hildebrandt F,Benmerah A

MA5-12080 was used in western blot to identify MAPKBP1 mutations as a genetic cause of juvenile or late-onset and cilia-independent nephronophthisis

Thu Feb 02 00:00:00 EST 2017

Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.

Journal of virology

DeRussy BM,Boland MT,Tandon R

MA5-12080 was used in immunoprecipitation to determine the link between nuclear egress and nucleocapsid maturation by human cytomegalovirus pUL93

Mon Aug 15 00:00:00 EDT 2016

YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process.

Oncotarget

Leal MF,Ribeiro HF,Rey JA,Pinto GR,Smith MC,Moreira-Nunes CA,Assumpção PP,Lamarão LM,Calcagno DQ,Montenegro RC,Burbano RR

Published figure using c-Myc monoclonal antibody (Product # MA5-12080) in Western Blot

Tue Dec 20 00:00:00 EST 2016

CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation.

PloS one

Ramena G,Yin Y,Yu Y,Walia V,Elble RC

MA5-12080 was used in immunocytochemistry, immunoprecipitation, and western blot to study the requirement of CLCA2 interactor EVA1 for mammary epithelial cell differentiation

Thu Jul 21 00:00:00 EDT 2016

BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.

Cancer genetics

Akay OM,Aras BD,Isiksoy S,Toprak C,Mutlu FS,Artan S,Oner U,Gulbas Z

MA5-12080 was used in immunohistochemistry to study the prognostic value of immunohistochemical and FISH analysis of BCL2, BCL6, IGH, TP53, and MYC in Turkish patients with diffuse large B-cell lymphoma

Sat Mar 01 00:00:00 EST 2014

MYC expression and distribution in normal mature lymphoid cells.

The Journal of pathology

Cattoretti G

MA5-12080 was used in western blot to study which normal mature lymphoid cells express myc

Fri Feb 01 00:00:00 EST 2013

The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.

International journal of oncology

Unger C,Popescu R,Giessrigl B,Laimer D,Heider S,Seelinger M,Diaz R,Wallnöfer B,Egger G,Hassler M,Knöfler M,Saleh L,Sahin E,Grusch M,Fritzer-Szekeres M,Dolznig H,Frisch R,Kenner L,Kopp B,Krupitza G

MA5-12080 was used in western blot to study the role of reduced NPM/ALK expression in the potential therapeutic mechanism of an ethnomedical plant extract against anaplastic large cell lymphoma

Tue Jan 01 00:00:00 EST 2013

EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.

Journal of cutaneous pathology

Jacobs MS,Persons DL,Fraga GR

MA5-12080 was used in immunohistochemistry to use FISH to study the higher frequency of EGFR and MYC gene amplification in squamous cell carcinoma than in keratoacanthoma

Wed May 01 00:00:00 EDT 2013

Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.

Molecular and cellular biology

Huang YS,Chiang NY,Hu CH,Hsiao CC,Cheng KF,Tsai WP,Yona S,Stacey M,Gordon S,Chang GW,Lin HH

MA5-12080 was used in western blot to demonstrate a critical role for the GPCR proteolysis site in autoproteolysis that mediates receptor signaling and cell activation.

Sun Apr 01 00:00:00 EDT 2012

Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.

Pathology oncology research : POR

Giaginis C,Politi E,Alexandrou P,Sfiniadakis J,Kouraklis G,Theocharis S

MA5-12080 was used in immunohistochemistry (paraffin) to determine the clinical significance of PPAR-γ expression in non-small cell lung carcinoma.

Mon Oct 01 00:00:00 EDT 2012

Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.

PloS one

Novellino L,Rossi RL,Bonino F,Cavallone D,Abrignani S,Pagani M,Brunetto MR

MA5-12080 was used in immunoprecipitation to investigate if subviral HBsAg particles are released into the bloodstream within exosomes and microvescicles.

Fri Aug 03 00:00:00 EDT 2012

Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.

Journal of virology

Genovese NJ,Broker TR,Chow LT

MA5-12080 was used in western blot to study the effect of non-conserved lysine residues on the function of human papillomavirus E7 protein

Wed Jun 01 00:00:00 EDT 2011

Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.

Molecular and cellular biology

Challagundla KB,Sun XX,Zhang X,DeVine T,Zhang Q,Sears RC,Dai MS

MA5-12080 was used in western blot to investigate the relationship between c-Myc and ribosomal biogenesis.

Sat Oct 01 00:00:00 EDT 2011

Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.

Pathology oncology research : POR

Li N,Wang J,Shen S,Bu X,Tian X,Huang P

MA5-12080 was used in immunohistochemistry to study the value of p53, Ki-67 and c-myc expression in determining the surgical margin in colorectal carcinoma resection

Thu Sep 01 00:00:00 EDT 2011

A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.

PloS one

Lanham KA,Prasch AL,Weina KM,Peterson RE,Heideman W

MA5-12080 was used in western blot to study the role of a dominant negative zebrafish Ahr2 in protecting zebrafish from dioxin toxicity

Mon Aug 27 00:00:00 EDT 2012

Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.

Carcinogenesis

Vo NT,Madlener S,Bago-Horvath Z,Herbacek I,Stark N,Gridling M,Probst P,Giessrigl B,Bauer S,Vonach C,Saiko P,Grusch M,Szekeres T,Fritzer-Szekeres M,Jäger W,Krupitza G,Soleiman A

MA5-12080 was used in western blot to investigate the mechanism for the biphasic effect of piceatannol on carcinogenesis

Wed Dec 01 00:00:00 EST 2010

Detection of protein-RNA complexes in Xenopus oocytes.

Methods (San Diego, Calif.)

Huber PW,Zhao WM

MA5-12080 was used in immunoprecipitation to develop a method for detecting protein-RNA complexes in Xenopus oocytes

Sat May 01 00:00:00 EDT 2010

Prognostic significance of c-Met expression in glioblastomas.

Cancer

Kong DS,Song SY,Kim DH,Joo KM,Yoo JS,Koh JS,Dong SM,Suh YL,Lee JI,Park K,Kim JH,Nam DH

MA512080 was used in immunohistochemistry - paraffin section to test if c-Met expression is associated with overall survival and tumor invasiveness in patients with glioblastomas

Thu Jan 01 00:00:00 EST 2009

NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes.

Journal of virology

Katzenellenbogen RA,Vliet-Gregg P,Xu M,Galloway DA

MA5-12080 was used in western blot to study the localization of NFX1-123 and elucidate its function

Wed Jul 01 00:00:00 EDT 2009

Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.

The FEBS journal

Pridgeon JW,Webber EA,Sha D,Li L,Chin LS

MA5-12080 was used in western blot to study the role of ubiquitin signaling in multiple cellular processes using a proteomic analysis

Thu Jan 01 00:00:00 EST 2009

Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.

Cellular immunology

Yang L,Shen L,Shao Y,Zhao Q,Zhang W

MA5-12080 was used in immunocytochemistry and western blot to study the function of human Fcalpha/microR.

Fri Jun 12 00:00:00 EDT 2009

MYC insertions in diffuse-type gastric adenocarcinoma.

Anticancer research

Calcagno DQ,Guimarães AC,Leal MF,Seabra AD,Khayat AS,Pontes TB,Assumpção PP,De Arruda Cardoso Smith M,Burbano RR

MA5-12080 was used in immunohistochemistry to evaluate the genomic alterations of MYC in diffuse-type gastric adenocarcinoma

Wed Jul 01 00:00:00 EDT 2009

Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.

The breast journal

Altintas S,Lambein K,Huizing MT,Braems G,Asjoe FT,Hellemans H,Van Marck E,Weyler J,Praet M,Van den Broecke R,Vermorken JB,Tjalma WA

MA5-12080 was used in immunohistochemistry to evaluate oncogenic markers with prognostic significance in breast ductal carcinoma in situ

Wed Jul 08 00:00:00 EDT 2009

Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.

Cancer cell

Itahana K,Zhang Y

MA5-12080 was used in western blot to examine the role of mitochondrial p32 in ARF-induced apoptosis

Sun Jun 01 00:00:00 EDT 2008

Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.

Clinical cancer research : an official journal of the American Association for Cancer Research

de Haas T,Hasselt N,Troost D,Caron H,Popovic M,Zadravec-Zaletel L,Grajkowska W,Perek M,Osterheld MC,Ellison D,Baas F,Versteeg R,Kool M

MA5-12080 was used in immunohistochemistry to develop a molecular risk stratification model for medulloblastoma patients

Tue Jul 01 00:00:00 EDT 2008

Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.

Lung cancer (Amsterdam, Netherlands)

Fokkema E,Timens W,de Vries EG,de Jong S,Fidler V,Meijer C,Groen HJ

MA5-12080 was used in immunohistochemistry to study the prognostic value of apoptosis-related protein expression in locally unresectable non-small cell lung cancers

Mon May 01 00:00:00 EDT 2006

Human melanocortin receptor 2 expression and functionality: effects of protein kinase A and protein kinase C on desensitization and internalization.

Endocrinology

Kilianova Z,Basora N,Kilian P,Payet MD,Gallo-Payet N

MA5-12080 was used in immunocytochemistry to study the short-term regulation of the ACTH receptor human melanocortin receptor 2.

Mon May 01 00:00:00 EDT 2006

Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.

Carcinogenesis

Mason DX,Keppler D,Zhang J,Jackson TJ,Seger YR,Matsui S,Abreo F,Cowell JK,Hannon GJ,Lowe SW,Lin AW

MA5-12080 was used in western blot to study the genetic events associated with spontaneous in vitro transformation of EIA/Ras-expressing human fibroblasts

Wed Feb 01 00:00:00 EST 2006

The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.

The Biochemical journal

Väisänen MR,Väisänen T,Tu H,Pirilä P,Sormunen R,Pihlajaniemi T

MA5-12080 was used in western blot to study the interplay between the shed soluble ectodomain of type XIII collagen and the fibrillar fibronectin matrix

Sun Jan 01 00:00:00 EST 2006

c-Myc-regulated microRNAs modulate E2F1 expression.

Nature

O'Donnell KA,Wentzel EA,Zeller KI,Dang CV,Mendell JT

MA5-12080 was used in western blot to investigate miRNAs and c-Myc.

Thu Jun 09 00:00:00 EDT 2005

Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.

Molecular and cellular biology

Talukder AH,Gururaj A,Mishra SK,Vadlamudi RK,Kumar R

MA5-12080 was used in immunoprecipitation to investigate the interaction between MTA1 and NRIF3

Sun Aug 01 00:00:00 EDT 2004

Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.

Molecular immunology

Bruno ME,West RB,Schneeman TA,Bresnick EH,Kaetzel CS

MA5-12080 was used in western blot to study an upstream regulatory factor that enhances expression of the human polymeric immunoglobulin receptor

Thu Jan 01 00:00:00 EST 2004

Transmembrane peptides as inhibitors of ErbB receptor signaling.

Molecular biology of the cell

Bennasroune A,Fickova M,Gardin A,Dirrig-Grosch S,Aunis D,Crémel G,Hubert P

MA5-12080 was used in western blot to investigate the interaction between transmembrane domain of receptor tyrosine kinases and ErbB receptor

Thu Jul 01 00:00:00 EDT 2004

Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.

The Journal of biological chemistry

Wei J,Sherman F

MA5-12080 was used in western blot to study the role of the nuclear encoded mitochondrial protein Sue1p in the degradation of altered holo-iso-1-cytochrome c isoforms in yeast mitochondria

Fri Jul 16 00:00:00 EDT 2004

A core function for p120-catenin in cadherin turnover.

The Journal of cell biology

Davis MA,Ireton RC,Reynolds AB

MA5-12080 was used in immunocytochemistry to study how p120-catenin stabilizes epithelial cadherin.

Mon Nov 10 00:00:00 EST 2003

AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.

Journal of molecular endocrinology

Kefas BA,Cai Y,Ling Z,Heimberg H,Hue L,Pipeleers D,Van de Casteele M

MA5-12080 was used in western blot to investigate the mechanisms involved in activated AMP-activated protein kinase-induced apoptosis.

Tue Apr 01 00:00:00 EST 2003

GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.

The Journal of experimental medicine

Baker-LePain JC,Sarzotti M,Fields TA,Li CY,Nicchitta CV

MA5-12080 was used in immunocytochemistry and immunoprecipitation to investigate the tissue restriction of GRP94-elicited protective immunity using a 4T1 mammary carcinoma model.

Mon Dec 02 00:00:00 EST 2002

A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.

FEBS letters

Hitzl M,Striessnig J,Neuhuber B,Flucher BE

MA5-12080 was used in immunocytochemistry to examine how alpha(1) and beta subunits of voltage-gated Ca(2+) channels interact.

Wed Jul 31 00:00:00 EDT 2002

Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.

Journal of immunological methods

Piechocki MP,Pilon SA,Wei WZ

MA5-12080 was used in immunocytochemistry and western blot to develop anti-ErbB-2 antibody assays using flow cytometry and ELISA.

Tue Jan 01 00:00:00 EST 2002

Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation.

The Journal of biological chemistry

Chin LS,Vavalle JP,Li L

MA5-12080 was used in Immunoprecipitation to identify and characterise a novel RING finger protein that interacts with syntaxin 1.

Fri Sep 20 00:00:00 EDT 2002

PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells.

The EMBO journal

Castoria G,Migliaccio A,Bilancio A,Di Domenico M,de Falco A,Lombardi M,Fiorentino R,Varricchio L,Barone MV,Auricchio F

MA5-12080 was used in western blot to elucidate Src- and PI3-kinase-dependent pathways in oestradiol-treated cells.

Thu Nov 01 00:00:00 EST 2001

Early and persistent bone marrow hematopoiesis defect in simian/human immunodeficiency virus-infected macaques despite efficient reduction of viremia by highly active antiretroviral therapy during primary infection.

Journal of virology

Thiebot H,Louache F,Vaslin B,de Revel T,Neildez O,Larghero J,Vainchenker W,Dormont D,Le Grand R

MA5-12080 was used in immunocytochemistry to discuss macaque models of AIDS.

Sat Dec 01 00:00:00 EST 2001

Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Chavand O,Spilsbury K,Rakoczy PE

MA5-12080 was used in western blot to examine the effect of individual VEGF isoform overexpression in neovascular disease models.

Thu Jun 14 00:00:00 EDT 2001

The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells.

The Journal of biological chemistry

Fukumoto T,Watanabe-Fukunaga R,Fujisawa K,Nagata S,Fukunaga R

MA5-12080 was used in western blot to study the role of the Fu protein kinase in hedgehog stimulation.

Fri Oct 19 00:00:00 EDT 2001

Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.

Biochimica et biophysica acta

Suzuki M,Shinkai M,Honda H,Kamihira M,Iijima S,Kobayashi T

MA5-12080 was used in flow cytometry to develop tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody for drug targeting applications

Fri Feb 16 00:00:00 EST 2001

Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.

European journal of cancer (Oxford, England : 1990)

Bourgarel-Rey V,El Khyari S,Rimet O,Bordas B,Guigal N,Braguer D,Seree E,Barra Y,Briand C

MA5-12080 was used in immunocytochemistry and western blot to determine the expression of c-myc in cells treated with microtubule stabilizing or depolymerizing agents.

Mon May 01 00:00:00 EDT 2000

The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.

The Journal of biological chemistry

de Caestecker MP,Yahata T,Wang D,Parks WT,Huang S,Hill CS,Shioda T,Roberts AB,Lechleider RJ

MA5-12080 was used in immunoprecipitation to characterize the functional activity of the Smad4 activation domain.

Fri Jan 21 00:00:00 EST 2000

Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.

Molecular cell

Zhang Y,Xiong Y

MA5-12080 was used in immunocytochemistry to study the effect of mutations in exon 2 of human ARF on its nucleolar localization and ability to regulate nuclear export of MDM2 and p53

Sat May 01 00:00:00 EDT 1999

ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.

Cell

Zhang Y,Xiong Y,Yarbrough WG

MA5-12080 was used in immunocytochemistry to investigate the effect of ARF on p53 regulation and its mechanism

Fri Mar 20 00:00:00 EST 1998

Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.

Human molecular genetics

Cooper JK,Schilling G,Peters MF,Herring WJ,Sharp AH,Kaminsky Z,Masone J,Khan FA,Delanoy M,Borchelt DR,Dawson VL,Dawson TM,Ross CA

MA5-12080 was used in western blot to develop a cell culture model to study the N-terminal fragments of huntingtin with expanded glutamine repeats.

Fri May 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog

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Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC

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UniProt ID: (Human) P01106

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Entrez Gene ID: (Human) 4609

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding protein dimerization activity repressing transcription factor binding E-box binding basic helix-loop-helix transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation energy reserve metabolic process chromatin remodeling transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus cell cycle arrest Notch signaling pathway positive regulation of cell proliferation response to gamma radiation regulation of gene expression oxygen transport regulation of telomere maintenance negative regulation of stress-activated MAPK cascade cellular response to UV cellular response to drug response to drug negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation positive regulation of epithelial cell proliferation chromosome organization negative regulation of cell division canonical Wnt signaling pathway response to growth factor positive regulation of metanephric cap mesenchymal cell proliferation beta-catenin-TCF complex assembly positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus

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c-Myc Monoclonal Antibody (9E10.3)

c-Myc Antibody (MA5-12080) in ICC/IF

Product Details

MA5-12080

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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