Invitrogen

c-Myc Monoclonal Antibody (9E10), eBioscience™

c-Myc Antibody in Immunocytochemistry (ICC/IF)

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c-Myc Antibody (14-6784-82) in ICC/IF

Immunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with c-Myc Monoclonal Antibody (9E10) (Product # 14-6784-82) at 1:100 dilution and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L... View More

c-Myc Antibody in Immunohistochemistry (Paraffin) (IHC (P))

c-Myc Antibody in Immunoprecipitation (IP)

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Product Details

14-6784-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9E10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468351

Product Specific Information

Description: The 9E10 antibody reacts with human c-Myc p67; the antibody was raised against a synthetic peptide corresponding to amino acids 408-439 within the carboxy terminal domain of human c-Myc. This antibody can be used to detect myc-tagged protein. The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. C-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway. These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes.

Applications Reported: This 9E10 antibody has been reported for use in immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This 9E10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 9E10 antibody has also been tested by western blot and can be used 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clinical-scale expansion of CD34⁺ cord blood cells amplifies committed progenitors and rapid scid repopulation cells.

New biotechnology

Casamayor-Genescà A,Pla A,Oliver-Vila I,Pujals-Fonts N,Marín-Gallén S,Caminal M,Pujol-Autonell I,Carrascal J,Vives-Pi M,Garcia J,Vives J

14678482 was used in ELISA to optimize a method to generate granulocyte lineage-committed progenitors from umbilical cord blood samples

Sat Mar 25 00:00:00 EDT 2017

The NPC2 protein: A novel dog allergen.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology

Khurana T,Newman-Lindsay S,Young PR,Slater JE

14678482 was used in western blot to discuss dog allergens such as Can f 1

Sun May 01 00:00:00 EDT 2016

Increased Twist expression in advanced stage of mycosis fungoides and Sézary syndrome.

Journal of cutaneous pathology

Goswami M,Duvic M,Dougherty A,Ni X

14-6784 was used in Immunohistochemistry on paraffin embedded tissues to investigate whether Twist expression is correlated to tumour stages in mycosis fungoides and Sézary syndrome.

Tue May 01 00:00:00 EDT 2012

Select paramyxoviral V proteins inhibit IRF3 activation by acting as alternative substrates for inhibitor of kappaB kinase epsilon (IKKe)/TBK1.

The Journal of biological chemistry

Lu LL,Puri M,Horvath CM,Sen GC

14-6784 was used in Western Blotting to demonstrate that 3 viruses of the genus Rubulavirus encode V proteins that inhibit double-stranded RNA-mediated signaling.

Fri May 23 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog

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Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC

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UniProt ID: (Human) P01106

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Entrez Gene ID: (Human) 4609

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding protein dimerization activity repressing transcription factor binding E-box binding basic helix-loop-helix transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation energy reserve metabolic process chromatin remodeling transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus cell cycle arrest Notch signaling pathway positive regulation of cell proliferation response to gamma radiation regulation of gene expression oxygen transport regulation of telomere maintenance negative regulation of stress-activated MAPK cascade cellular response to UV cellular response to drug response to drug negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation positive regulation of epithelial cell proliferation chromosome organization negative regulation of cell division canonical Wnt signaling pathway response to growth factor positive regulation of metanephric cap mesenchymal cell proliferation beta-catenin-TCF complex assembly positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Myc Monoclonal Antibody (9E10), eBioscience™

c-Myc Antibody (14-6784-82) in ICC/IF

Product Details

14-6784-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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