Invitrogen

c-Myc Monoclonal Antibody (9E11)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Published Figures (3)

c-Myc Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

c-Myc Antibody (MA1-16637) in IHC (P)

Immunohistochemical analysis of c-Myc in immersion fixed paraffin-embedded sections of human breast cancer. Samples were incubated in c-Myc monoclonal antibody (Product # MA1-16637) using a dilution of 1:600 overnight at 4 °C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).

c-Myc Antibody in Immunocytochemistry (ICC/IF)

c-Myc Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Product Details

MA1-16637

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

View 1 publication 1 publication

ELISA (ELISA)

1:100-1:2,000

Immunoprecipitation (IP)

2 µg/mg lysate

View 6 publications 6 publications

ChIP assay (ChIP)

2 µg/500 µg extract

View 8 publications 8 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat, Yeast

Published species

Human, Mouse, Rat, Shrew, Yeast

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

9E11

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to residues A(408) E E Q K L I S E E D L(420) of human c-Myc.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568496

Product Specific Information

Suggested positive control: antigen standard for MYC (transient overexpression lysate), HL-60 cells or breast tumors, HL-60 whole cell lysate, human breast carcinoma whole cell lysate.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isolation, culture, and characterization of chicken intestinal epithelial cells.

BMC molecular and cell biology

Ghiselli F,Rossi B,Felici M,Parigi M,Tosi G,Fiorentini L,Massi P,Piva A,Grilli E

Published figure using c-Myc monoclonal antibody (Product # MA1-16637) in Immunocytochemistry

Fri Feb 12 00:00:00 EST 2021

Tupaia OASL1 Promotes Cellular Antiviral Immune Responses by Recruiting MDA5 to MAVS.

Journal of immunology (Baltimore, Md. : 1950)

Yao YL,Yu D,Xu L,Gu T,Li Y,Zheng X,Bi R,Yao YG

MA1-16637 was used in Flow cytometry/Cell sorting to identify the Tupaia oligoadenylate synthetases-like 1 (tOASL1) as a positive regulator of the Tupaia MDA5 (tMDA5) and Tupaia MAVS (tMAVS)-mediated IFN signaling.

Tue Dec 15 00:00:00 EST 2020

Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew.

Proceedings of the National Academy of Sciences of the United States of America

Xu L,Yu D,Fan Y,Peng L,Wu Y,Yao YG

MA116637 was used in western blot to investigate the evolution of RIG-I-like receptors

Tue Sep 27 00:00:00 EDT 2016

Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew.

Proceedings of the National Academy of Sciences of the United States of America

Xu L,Yu D,Fan Y,Peng L,Wu Y,Yao YG

MA116637 was used in western blot to investigate the evolution of RIG-I-like receptors

Tue Sep 27 00:00:00 EDT 2016

LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells.

Oncogene

Yokdang N,Hatakeyama J,Wald JH,Simion C,Tellez JD,Chang DZ,Swamynathan MM,Chen M,Murphy WJ,Carraway Iii KL,Sweeney C

MA1-16637 was used in western blot demonstrate that LRIG1 functions as a growth suppressor in breast cancer cells

Thu Jun 02 00:00:00 EDT 2016

MYC expression and distribution in normal mature lymphoid cells.

The Journal of pathology

Cattoretti G

MA1-16637 was used in western blot to study which normal mature lymphoid cells express myc

Fri Feb 01 00:00:00 EST 2013

Trypanosoma brucei 14-3-3I and II proteins predominantly form a heterodimer structure that acts as a potent cell cycle regulator in vivo.

Journal of biochemistry

Inoue M,Yasuda K,Uemura H,Yasaka N,Schnaufer A,Yano M,Kido H,Kohda D,Doi H,Fukuma T,Tsuji A,Horikoshi N

MA1-16637 was used in immunoprecipitation and western blot to study the cell cycle regulatory activity of heterodimers of Trypanosoma brucei 14-3-3 I and 14-3-3 II

Wed May 01 00:00:00 EDT 2013

Mcm10 self-association is mediated by an N-terminal coiled-coil domain.

PloS one

Du W,Josephrajan A,Adhikary S,Bowles T,Bielinsky AK,Eichman BF

MA1-16637 was used in western blot to study the role of the N-terminal coiled-coil domain in the self-association and oligomerization of Xenopus Mcm10

Thu Mar 27 00:00:00 EDT 2014

The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast.

PloS one

Yeheskely-Hayon D,Kotler A,Stark M,Hashimshony T,Sagee S,Kassir Y

MA1-16637 was used in ChIP assay to study the regulation of yeast gene transcription and the catalytic and non-catalytic roles of Rpd3L and Rpd3S

Tue Sep 16 00:00:00 EDT 2014

Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

The EMBO journal

Kanke M,Kodama Y,Takahashi TS,Nakagawa T,Masukata H

MA1-16637 was used in ChIP assay to study the essential role of Mcm10 in origin DNA unwinding

Wed May 02 00:00:00 EDT 2012

First-in-class small molecule inhibitors of the single-strand DNA cytosine deaminase APOBEC3G.

ACS chemical biology

Li M,Shandilya SM,Carpenter MA,Rathore A,Brown WL,Perkins AL,Harki DA,Solberg J,Hook DJ,Pandey KK,Parniak MA,Johnson JR,Krogan NJ,Somasundaran M,Ali A,Schiffer CA,Harris RS

MA1-16637 was used in western blot to investigate the mechanism of APOBEC3G inhibitors through structural analysis

Fri Mar 16 00:00:00 EDT 2012

Widespread mRNA association with cytoskeletal motor proteins and identification and dynamics of myosin-associated mRNAs in S. cerevisiae.

PloS one

Casolari JM,Thompson MA,Salzman J,Champion LM,Moerner WE,Brown PO

MA1-16637 was used in ChIP assay to study mRNAs associated with cytoskeletal motor proteins in Saccharomyces cerevisiae

Fri Aug 03 00:00:00 EDT 2012

Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions.

BMC genomics

Milliman EJ,Hu Z,Yu MC

MA1-16637 was used in ChIP assay and immunoprecipitation to identify novel roles of the protein arginine methyltransferase Hmt in regulating eukaryotic gene expression

Wed Dec 26 00:00:00 EST 2012

Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions.

BMC genomics

Milliman EJ,Hu Z,Yu MC

MA1-16637 was used in ChIP assay and immunoprecipitation to identify novel roles of the protein arginine methyltransferase Hmt in regulating eukaryotic gene expression

Wed Dec 26 00:00:00 EST 2012

Recruitment of Rpd3 to the telomere depends on the protein arginine methyltransferase Hmt1.

PloS one

Milliman EJ,Yadav N,Chen YC,Muddukrishna B,Karunanithi S,Yu MC

MA1-16637 was used in ChIP assay to study the role of the Hmt1 protein arginine methyltransferase in the telomeric recruitment of Rpd3

Thu Feb 07 00:00:00 EST 2013

CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.

Molecular biology of the cell

Fukuura M,Nagao K,Obuse C,Takahashi TS,Nakagawa T,Masukata H

MA1-16637 was used in ChIP assay to study the mechanism by which CDK promotes the initiation of DNA replication in fission yeast

Fri Jul 15 00:00:00 EDT 2011

Auxin-inducible protein depletion system in fission yeast.

BMC cell biology

Kanke M,Nishimura K,Kanemaki M,Kakimoto T,Takahashi TS,Nakagawa T,Masukata H

MA1-16637 was used in western blot to construct a useful protein-depletion system for Shizosaccaromyces pombe

Fri Feb 11 00:00:00 EST 2011

Defects in DNA ligase I trigger PCNA ubiquitylation at Lys 107.

Nature cell biology

Das-Bradoo S,Nguyen HD,Wood JL,Ricke RM,Haworth JC,Bielinsky AK

MA1-16637 was used in immunoprecipitation to study DNA ligase I deficiencies and their effects on repair of DNA damage

Fri Jan 01 00:00:00 EST 2010

The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repair.

Molecular biology of the cell

Sollier J,Driscoll R,Castellucci F,Foiani M,Jackson SP,Branzei D

MA1-16637 was used in immunoprecipitation and western blot to study the role of Ecs2 and Smc5-6 in yeast sister chromatid junction-mediated intra-S repair

Sun Mar 01 00:00:00 EST 2009

Structural basis for DNA binding by replication initiator Mcm10.

Structure (London, England : 1993)

Warren EM,Vaithiyalingam S,Haworth J,Greer B,Bielinsky AK,Chazin WJ,Eichman BF

MA1-16637 was used in western blot to study the structural basis for DNA binding by replication initiator Mcm10

Wed Dec 10 00:00:00 EST 2008

A Sveinsson's chorioretinal atrophy-associated missense mutation in mouse Tead1 affects its interaction with the co-factors YAP and TAZ.

Biochemical and biophysical research communications

Kitagawa M

MA1-16637 was used in western blot to study the pathological mechanism of a Sveinsson's chorioretinal atrophy-associated missense mutation in mouse Tead1

Fri Oct 05 00:00:00 EDT 2007

Protein-tyrosine phosphatase PCP-2 inhibits beta-catenin signaling and increases E-cadherin-dependent cell adhesion.

The Journal of biological chemistry

Yan HX,Yang W,Zhang R,Chen L,Tang L,Zhai B,Liu SQ,Cao HF,Man XB,Wu HP,Wu MC,Wang HY

MA1-16637 was used in western blot to investigate the importance of protein-tyrosine phosphatase PCP-2 in the regulation of beta-catenin signaling

Fri Jun 02 00:00:00 EDT 2006

A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast.

The Journal of biological chemistry

Ricke RM,Bielinsky AK

MA1-16637 was used in western blot to study the role of a conserved Hsp10-like domain of Mcm10 in stabilizing DNA polymerase alpha in budding yeast

Fri Jul 07 00:00:00 EDT 2006

Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.

Cell

Casolari JM,Brown CR,Komili S,West J,Hieronymus H,Silver PA

MA1-16637 was used in ChIP assay to study the association of genome-wide localization of the nuclear transport machinery with transcriptional status and nuclear organization

Fri May 14 00:00:00 EDT 2004

Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.

Molecular cell

Ricke RM,Bielinsky AK

MA1-16637 was used in immunoprecipitation to study the role of the DNA replication factor Mcm10 in regulating DNA polymerase alpha stability and chromatin association

Fri Oct 22 00:00:00 EDT 2004

Activin isoforms signal through type I receptor serine/threonine kinase ALK7.

Molecular and cellular endocrinology

Tsuchida K,Nakatani M,Yamakawa N,Hashimoto O,Hasegawa Y,Sugino H

MA1-16637 was used in western blot to study the role of the Type I receptor serine/threonine kinase ALK7 in activin signaling

Mon May 31 00:00:00 EDT 2004

Low expression levels of p27 correlate with loco-regional recurrence in nasopharyngeal carcinoma.

Cancer letters

Hwang CF,Su CY,Huang SC,Huang CC,Fang FM,Lui CC,Chang HW,Cho CL

MA1-16637 was used in immunohistochemistry to investigate the relationship between p27 level and nasopharyngeal carcinoma recurrence

Tue Jan 28 00:00:00 EST 2003

Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing.

Nature genetics

Kimura A,Umehara T,Horikoshi M

MA1-16637 was used in ChIP assay to study the roles of Sas2p and Sir2p in histone acetylation and gene expression

Fri Nov 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-Myc; c-myc epitope tag; c-myc proto-oncogene; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; MYC proto-oncogene, bHLH transcription factor; myc-related translation/localization regulatory factor; myelocytomatosis viral oncogene homolog; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene-like protein

View more View less

Gene Aliases: AU016757; BHLHE39; c-Myc; mMyc; MRTL; MYC; Myc2; MYCC; Niard; Nird; RNCMYC

View more View less

UniProt ID: (Chicken) P01109, (Human) P01106, (Rat) P09416, (Mouse) P01108

View more View less

Entrez Gene ID: (Chicken) 420332, (Human) 4609, (Rat) 24577, (Mouse) 17869

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein dimerization activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding repressing transcription factor binding E-box binding core promoter proximal region sequence-specific DNA binding double-stranded DNA binding sequence-specific DNA binding protein heterodimerization activity basic helix-loop-helix transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

c-Myc Monoclonal Antibody (9E11)

c-Myc Antibody (MA1-16637) in IHC (P)

Product Details

MA1-16637

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!