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cIAP2 Antibody (PA5-19514) in ICC/IF
Immunofluorescent staining of HeLa cells using Product # PA5-19514, anti-cIAP2 antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
Product Details
PA5-19514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human cIAP2.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10986934
Product Specific Information
For Western Blot, this antibody has non-specific bands at 150 kDa,35 kDa and 75 kDa.
This antibody is predicted to react with mouse, rat, chicken and dog based on sequence homology.
Target Information
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The amino acid sequence predicts three baculovirus IAP repeat domains and a ring finger domain. Transcript variants encoding the same isoform have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIP 2; API2; Apoptosis inhibitor 2; baculoviral IAP repeat-containing 3; Baculoviral IAP repeat-containing protein 3; C-IAP2; Cellular inhibitor of apoptosis 2; H HIAP1; H MALT2; hIAP-1; IAP homolog; IAP homolog C; IAP-1; Inhibitor of apoptosis protein 1; mammalian IAP homolog C; RING finger protein 49; RING-type E3 ubiquitin transferase BIRC3; TNFR2-TRAF signaling complex protein; TNFR2-TRAF-signaling complex protein 1
Gene Aliases: AIP1; API2; Birc2; BIRC3; c-IAP2; CIAP2; HAIP1; HIAP1; IAP1; MALT2; MIHC; RNF49
UniProt ID: (Human) Q13489
Entrez Gene ID: (Human) 330, (Rat) 78971
apoptotic process
cell surface receptor signaling pathway
I-kappaB kinase/NF-kappaB signaling
spermatogenesis
regulation of tumor necrosis factor-mediated signaling pathway
regulation of necrotic cell death
protein ubiquitination
positive regulation of protein ubiquitination
tumor necrosis factor-mediated signaling pathway
regulation of toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
NIK/NF-kappaB signaling
regulation of RIG-I signaling pathway
regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of innate immune response
regulation of inflammatory response
protein heterooligomerization
regulation of necroptotic process
negative regulation of necroptotic process
regulation of nucleotide-binding oligomerization domain containing signaling pathway
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cysteine-type endopeptidase activity
negative regulation of phosphorylation
necroptotic process
negative regulation of reactive oxygen species metabolic process
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