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eIF4E Antibody (MA5-37479) in ICC/IF
Immunocytochemistry/Immunofluorescent analysis of eIF4E in Hela cells stained with eIF4E Monoclonal Antibody (Product # MA5-37479). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with eIF4E primary antibody (1:25, 1h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1h at 37°C). eIF4E immunoreactivity i... View More
Product Details
MA5-37479
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
163CT48.1.9
Immunogen
EIF4E recombinant protein
Conjugate
Form
Liquid
Concentration
0.5
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897412
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705
Gene Aliases: AUTS19; CBP; EG668879; eIF-4E; EIF4E; Eif4e-ps; EIF4E1; EIF4EL1; EIF4F; If4e
UniProt ID: (Human) P06730, (Mouse) P63073
Entrez Gene ID: (Human) 1977, (Mouse) 13684
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA poly(A) tail shortening
behavioral fear response
RNA export from nucleus
mRNA export from nucleus
translational initiation
regulation of translation
viral process
negative regulation of translation
stem cell population maintenance
lung development
negative regulation of neuron differentiation
positive regulation of mitotic cell cycle
translation
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