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eIF6 Antibody (PA5-79197) in ICC/IF
Immunocytochemistry analysis of eIF6 on SMMC-7721 cells. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with eIF6 polyclonal antibody (Product# PA5-79197) with a dilution of 1 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Product Details
PA5-79197
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human EIF6 recombinant protein (Position: N66-T210).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746313
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B(2)GCN homolog; B4 integrin interactor; CAB; eIF-6; eIF-6 {ECO:0000255|HAMAP-Rule:MF_03132}; EIF6; eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}; integrin beta 4 binding protein; p27 beta-4 integrin-binding protein; p27(BBP)
Gene Aliases: 1110004P11; AA408895; b(2)gcn; CAB; eIF-6; EIF3A; EIF6; imc-415; ITGB4BP; OK/SW-cl.27; p27(BBP); p27BBP
UniProt ID: (Human) P56537, (Rat) Q3KRD8, (Mouse) O55135
Entrez Gene ID: (Human) 3692, (Rat) 305506, (Mouse) 16418
ribosomal subunit export from nucleus
maturation of 5.8S rRNA
maturation of LSU-rRNA
regulation of glycolytic process
translational initiation
response to insulin
gene silencing by miRNA
miRNA mediated inhibition of translation
mature ribosome assembly
regulation of fatty acid biosynthetic process
regulation of megakaryocyte differentiation
positive regulation of translation
assembly of large subunit precursor of preribosome
regulation of reactive oxygen species metabolic process
ribosomal large subunit biogenesis
translation
ribosome biogenesis
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